Functional Characteristics Analysis of Dehydrins in <i>Larix kaempferi</i> under Osmotic Stress
Dehydrins (DHN) belong to the late embryogenesis abundant II family and have been found to enhance plant tolerance to abiotic stress. In the present study, we reported four DHNs in <i>Larix kaempferi</i> (LkDHN) which were identified from the published transcriptome. Alignment analysis s...
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/4/1715 |
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| author | Xuechun Wang Meng Zhang Baohui Xie Xiangning Jiang Ying Gai |
| author_facet | Xuechun Wang Meng Zhang Baohui Xie Xiangning Jiang Ying Gai |
| author_sort | Xuechun Wang |
| collection | DOAJ |
| description | Dehydrins (DHN) belong to the late embryogenesis abundant II family and have been found to enhance plant tolerance to abiotic stress. In the present study, we reported four DHNs in <i>Larix kaempferi</i> (LkDHN) which were identified from the published transcriptome. Alignment analysis showed that these four LkDHNs shared close relationships and belonged to SK<sub>3</sub>-type DHNs. The electrophoretic mobility shift assay indicated that these four LkDHNs all possess sequence-independent binding capacity for double-strands DNAs. The subcellular localizations of the four LkDHNs were in both the nucleus and cytoplasm, indicating that these LkDHNs enter the nucleus to exert the ability to bind DNA. The preparation of tobacco protoplasts with different concentrations of mannitol showed that LkDHNs enhanced the tolerance of plant cells under osmotic stress. The overexpression of LkDHNs in yeasts enhanced their tolerance to osmotic stress and helped the yeasts to survive severe stress. In addition, LkDHNs in the nucleus of salt treated tobacco increased. All of these results indicated that the four LkDHNs help plants survive from heavy stress by participating in DNA protection. These four LKDHNs played similar roles in the response to osmotic stress and assisted in the adaptation of <i>L</i>. <i>kaempferi</i> to the arid and cold winter of northern China. |
| first_indexed | 2024-03-09T05:03:40Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:03:40Z |
| publishDate | 2021-02-01 |
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| spelling | doaj.art-10989be04b5949f092dd3612b93c892c2023-12-03T12:57:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224171510.3390/ijms22041715Functional Characteristics Analysis of Dehydrins in <i>Larix kaempferi</i> under Osmotic StressXuechun Wang0Meng Zhang1Baohui Xie2Xiangning Jiang3Ying Gai4College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaDehydrins (DHN) belong to the late embryogenesis abundant II family and have been found to enhance plant tolerance to abiotic stress. In the present study, we reported four DHNs in <i>Larix kaempferi</i> (LkDHN) which were identified from the published transcriptome. Alignment analysis showed that these four LkDHNs shared close relationships and belonged to SK<sub>3</sub>-type DHNs. The electrophoretic mobility shift assay indicated that these four LkDHNs all possess sequence-independent binding capacity for double-strands DNAs. The subcellular localizations of the four LkDHNs were in both the nucleus and cytoplasm, indicating that these LkDHNs enter the nucleus to exert the ability to bind DNA. The preparation of tobacco protoplasts with different concentrations of mannitol showed that LkDHNs enhanced the tolerance of plant cells under osmotic stress. The overexpression of LkDHNs in yeasts enhanced their tolerance to osmotic stress and helped the yeasts to survive severe stress. In addition, LkDHNs in the nucleus of salt treated tobacco increased. All of these results indicated that the four LkDHNs help plants survive from heavy stress by participating in DNA protection. These four LKDHNs played similar roles in the response to osmotic stress and assisted in the adaptation of <i>L</i>. <i>kaempferi</i> to the arid and cold winter of northern China.https://www.mdpi.com/1422-0067/22/4/1715dehydrinosmotic stressDNA binding<i>Larix kaempferi</i> |
| spellingShingle | Xuechun Wang Meng Zhang Baohui Xie Xiangning Jiang Ying Gai Functional Characteristics Analysis of Dehydrins in <i>Larix kaempferi</i> under Osmotic Stress International Journal of Molecular Sciences dehydrin osmotic stress DNA binding <i>Larix kaempferi</i> |
| title | Functional Characteristics Analysis of Dehydrins in <i>Larix kaempferi</i> under Osmotic Stress |
| title_full | Functional Characteristics Analysis of Dehydrins in <i>Larix kaempferi</i> under Osmotic Stress |
| title_fullStr | Functional Characteristics Analysis of Dehydrins in <i>Larix kaempferi</i> under Osmotic Stress |
| title_full_unstemmed | Functional Characteristics Analysis of Dehydrins in <i>Larix kaempferi</i> under Osmotic Stress |
| title_short | Functional Characteristics Analysis of Dehydrins in <i>Larix kaempferi</i> under Osmotic Stress |
| title_sort | functional characteristics analysis of dehydrins in i larix kaempferi i under osmotic stress |
| topic | dehydrin osmotic stress DNA binding <i>Larix kaempferi</i> |
| url | https://www.mdpi.com/1422-0067/22/4/1715 |
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