222 nm far-UVC efficiently introduces nerve damage in Caenorhabditis elegans.
Far-ultraviolet radiation C light (far-UVC; 222 nm wavelength) has received attention as a safer light for killing pathogenic bacteria and viruses, as no or little DNA damage is observed after irradiation in mammalian skin models. Far-UVC does not penetrate deeply into tissues; therefore, it cannot...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0281162 |
| _version_ | 1797862082407825408 |
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| author | Kaoru Okamoto Yoshiyama Norihiko L Okamoto Jun Hidema Atsushi Higashitani |
| author_facet | Kaoru Okamoto Yoshiyama Norihiko L Okamoto Jun Hidema Atsushi Higashitani |
| author_sort | Kaoru Okamoto Yoshiyama |
| collection | DOAJ |
| description | Far-ultraviolet radiation C light (far-UVC; 222 nm wavelength) has received attention as a safer light for killing pathogenic bacteria and viruses, as no or little DNA damage is observed after irradiation in mammalian skin models. Far-UVC does not penetrate deeply into tissues; therefore, it cannot reach the underlying critical basal cells. However, it was unclear whether far-UVC (222-UVC) irradiation could cause more biological damage at shallower depths than the 254 nm UVC irradiation (254-UVC), which penetrates more deeply. This study investigated the biological effects of 222- and 254-UVC on the small and transparent model organism Caenorhabditis elegans. At the same energy level of irradiation, 222-UVC introduced slightly less cyclobutane pyrimidine dimer damage to naked DNA in solution than 254-UVC. The survival of eggs laid during 0-4 h after irradiation showed a marked decrease with 254-UVC but not 222-UVC. In addition, defect of chromosomal condensation was observed in a full-grown oocyte by 254-UVC irradiation. In contrast, 222-UVC had a significant effect on the loss of motility of C. elegans. The sensory nervous system, which includes dopamine CEP and PVD neurons on the body surface, was severely damaged by 222-UVC, but not by the same dose of 254-UVC. Interestingly, increasing 254-UVC irradiation by about 10-fold causes similar damage to CEP neurons. These results suggest that 222-UVC is less penetrating, so energy transfer occurs more effectively in tissues near the surface, causing more severe damage than 254-UVC. |
| first_indexed | 2024-04-09T22:13:33Z |
| format | Article |
| id | doaj.art-109a65913e934a06b1eaea97e6fae570 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T22:13:33Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-109a65913e934a06b1eaea97e6fae5702023-03-23T05:32:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01181e028116210.1371/journal.pone.0281162222 nm far-UVC efficiently introduces nerve damage in Caenorhabditis elegans.Kaoru Okamoto YoshiyamaNorihiko L OkamotoJun HidemaAtsushi HigashitaniFar-ultraviolet radiation C light (far-UVC; 222 nm wavelength) has received attention as a safer light for killing pathogenic bacteria and viruses, as no or little DNA damage is observed after irradiation in mammalian skin models. Far-UVC does not penetrate deeply into tissues; therefore, it cannot reach the underlying critical basal cells. However, it was unclear whether far-UVC (222-UVC) irradiation could cause more biological damage at shallower depths than the 254 nm UVC irradiation (254-UVC), which penetrates more deeply. This study investigated the biological effects of 222- and 254-UVC on the small and transparent model organism Caenorhabditis elegans. At the same energy level of irradiation, 222-UVC introduced slightly less cyclobutane pyrimidine dimer damage to naked DNA in solution than 254-UVC. The survival of eggs laid during 0-4 h after irradiation showed a marked decrease with 254-UVC but not 222-UVC. In addition, defect of chromosomal condensation was observed in a full-grown oocyte by 254-UVC irradiation. In contrast, 222-UVC had a significant effect on the loss of motility of C. elegans. The sensory nervous system, which includes dopamine CEP and PVD neurons on the body surface, was severely damaged by 222-UVC, but not by the same dose of 254-UVC. Interestingly, increasing 254-UVC irradiation by about 10-fold causes similar damage to CEP neurons. These results suggest that 222-UVC is less penetrating, so energy transfer occurs more effectively in tissues near the surface, causing more severe damage than 254-UVC.https://doi.org/10.1371/journal.pone.0281162 |
| spellingShingle | Kaoru Okamoto Yoshiyama Norihiko L Okamoto Jun Hidema Atsushi Higashitani 222 nm far-UVC efficiently introduces nerve damage in Caenorhabditis elegans. PLoS ONE |
| title | 222 nm far-UVC efficiently introduces nerve damage in Caenorhabditis elegans. |
| title_full | 222 nm far-UVC efficiently introduces nerve damage in Caenorhabditis elegans. |
| title_fullStr | 222 nm far-UVC efficiently introduces nerve damage in Caenorhabditis elegans. |
| title_full_unstemmed | 222 nm far-UVC efficiently introduces nerve damage in Caenorhabditis elegans. |
| title_short | 222 nm far-UVC efficiently introduces nerve damage in Caenorhabditis elegans. |
| title_sort | 222 nm far uvc efficiently introduces nerve damage in caenorhabditis elegans |
| url | https://doi.org/10.1371/journal.pone.0281162 |
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