Antiviral activity of red algae phycocolloids against herpes simplex virus type 2 in vitro
Herpes simplex virus type 2 (HSV-2) is a human infectious agent with significant impact on public health due to its high prevalence in the population and its ability to elicit a wide range of diseases, from mild to severe. Although several antiviral drugs, such as acyclovir, are currently available...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X23000188 |
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| author | Adolfo Henríquez Juan Pablo Vargas Chris Landahur Nicolás Corrales Andrés Agurto-Muñoz Pablo A. González Cristian Agurto-Muñoz |
| author_facet | Adolfo Henríquez Juan Pablo Vargas Chris Landahur Nicolás Corrales Andrés Agurto-Muñoz Pablo A. González Cristian Agurto-Muñoz |
| author_sort | Adolfo Henríquez |
| collection | DOAJ |
| description | Herpes simplex virus type 2 (HSV-2) is a human infectious agent with significant impact on public health due to its high prevalence in the population and its ability to elicit a wide range of diseases, from mild to severe. Although several antiviral drugs, such as acyclovir, are currently available to treat HSV-2-related clinical manifestations, their effectiveness is poor. Therefore, the identification and development of new antiviral drugs against HSV-2 is necessary. Seaweeds are attractive candidates for such purposes because they are a vast source of natural products due to their highly diverse compounds, many with demonstrated biological activity. In this study, we evaluated the in vitro antiviral potential of red algae extracts obtained from Agarophyton chilense, Mazzaella laminarioides, Porphyridium cruentum, and Porphyridium purpureum against HSV-2. The phycocolloids agar and carrageenan obtained from the macroalgae dry biomass of A. chilense and M. laminarioides and the exopolysaccharides from P. cruentum and P. purpureum were evaluated. The cytotoxicity of these extracts and the surpluses obtained in the extraction process of the agar and carrageenans were evaluated in human epithelial cells (HeLa cells) in addition to their antiviral activity against HSV-2, which were used to calculate selectivity indexes (SIs). Several compounds displayed antiviral activity against HSV-2, but carrageenans were not considered as a potential antiviral therapeutic agent when compared to the other algae extracts with a SI of 23.3. Future assays in vivo models for HSV-2 infection should reveal the therapeutic potential of these algae compounds as new antivirals against this virus. |
| first_indexed | 2024-03-13T07:42:27Z |
| format | Article |
| id | doaj.art-109e03537d80482b9e50d760403cc3d5 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-03-13T07:42:27Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-109e03537d80482b9e50d760403cc3d52023-06-03T04:22:12ZengElsevierBiotechnology Reports2215-017X2023-06-0138e00798Antiviral activity of red algae phycocolloids against herpes simplex virus type 2 in vitroAdolfo Henríquez0Juan Pablo Vargas1Chris Landahur2Nicolás Corrales3Andrés Agurto-Muñoz4Pablo A. González5Cristian Agurto-Muñoz6Grupo Interdisciplinario de Biotecnología Marina (GIBMAR), Centro de Biotecnología, Universidad de Concepción, Concepción, Chile; Departamento de Química Analítica e Inorgánica, Facultad de Ciencias Químicas, Universidad de Concepción, Concepción, Chile; Corresponding authors.Grupo Interdisciplinario de Biotecnología Marina (GIBMAR), Centro de Biotecnología, Universidad de Concepción, Concepción, ChileGrupo Interdisciplinario de Biotecnología Marina (GIBMAR), Centro de Biotecnología, Universidad de Concepción, Concepción, ChileMillennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, ChileGrupo Interdisciplinario de Biotecnología Marina (GIBMAR), Centro de Biotecnología, Universidad de Concepción, Concepción, ChileMillennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, ChileGrupo Interdisciplinario de Biotecnología Marina (GIBMAR), Centro de Biotecnología, Universidad de Concepción, Concepción, Chile; Departamento de Ciencia y Tecnología de los Alimentos, CyTA. Facultad de Farmacia. Universidad de Concepción, Concepción, Chile; Corresponding authors.Herpes simplex virus type 2 (HSV-2) is a human infectious agent with significant impact on public health due to its high prevalence in the population and its ability to elicit a wide range of diseases, from mild to severe. Although several antiviral drugs, such as acyclovir, are currently available to treat HSV-2-related clinical manifestations, their effectiveness is poor. Therefore, the identification and development of new antiviral drugs against HSV-2 is necessary. Seaweeds are attractive candidates for such purposes because they are a vast source of natural products due to their highly diverse compounds, many with demonstrated biological activity. In this study, we evaluated the in vitro antiviral potential of red algae extracts obtained from Agarophyton chilense, Mazzaella laminarioides, Porphyridium cruentum, and Porphyridium purpureum against HSV-2. The phycocolloids agar and carrageenan obtained from the macroalgae dry biomass of A. chilense and M. laminarioides and the exopolysaccharides from P. cruentum and P. purpureum were evaluated. The cytotoxicity of these extracts and the surpluses obtained in the extraction process of the agar and carrageenans were evaluated in human epithelial cells (HeLa cells) in addition to their antiviral activity against HSV-2, which were used to calculate selectivity indexes (SIs). Several compounds displayed antiviral activity against HSV-2, but carrageenans were not considered as a potential antiviral therapeutic agent when compared to the other algae extracts with a SI of 23.3. Future assays in vivo models for HSV-2 infection should reveal the therapeutic potential of these algae compounds as new antivirals against this virus.http://www.sciencedirect.com/science/article/pii/S2215017X23000188Agarophyton chilenseMazzaella laminarioidesPorphyridium cruentumPorphyridium purpureumAntiviral activityHerpes simplex type 2 |
| spellingShingle | Adolfo Henríquez Juan Pablo Vargas Chris Landahur Nicolás Corrales Andrés Agurto-Muñoz Pablo A. González Cristian Agurto-Muñoz Antiviral activity of red algae phycocolloids against herpes simplex virus type 2 in vitro Biotechnology Reports Agarophyton chilense Mazzaella laminarioides Porphyridium cruentum Porphyridium purpureum Antiviral activity Herpes simplex type 2 |
| title | Antiviral activity of red algae phycocolloids against herpes simplex virus type 2 in vitro |
| title_full | Antiviral activity of red algae phycocolloids against herpes simplex virus type 2 in vitro |
| title_fullStr | Antiviral activity of red algae phycocolloids against herpes simplex virus type 2 in vitro |
| title_full_unstemmed | Antiviral activity of red algae phycocolloids against herpes simplex virus type 2 in vitro |
| title_short | Antiviral activity of red algae phycocolloids against herpes simplex virus type 2 in vitro |
| title_sort | antiviral activity of red algae phycocolloids against herpes simplex virus type 2 in vitro |
| topic | Agarophyton chilense Mazzaella laminarioides Porphyridium cruentum Porphyridium purpureum Antiviral activity Herpes simplex type 2 |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X23000188 |
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