Assessment of Seasonal and Diurnal Variations of Alkaline Phosphatase Activity in Pasteurized Milk
The present study was conducted to detect the concentration levels of ALP (alkaline phosphatase) in pasteurized milk and determine whether the pasteurization was successful or not, according to WHO Directives, which clearly state that the ALP (alkaline phosphatase) substance in pasteurized milk must...
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| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/10/4833 |
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| author | Vaia Gerokomou Georgios Rozos Panagiotis Demertzis Konstantoula Akrida-Demertzi |
| author_facet | Vaia Gerokomou Georgios Rozos Panagiotis Demertzis Konstantoula Akrida-Demertzi |
| author_sort | Vaia Gerokomou |
| collection | DOAJ |
| description | The present study was conducted to detect the concentration levels of ALP (alkaline phosphatase) in pasteurized milk and determine whether the pasteurization was successful or not, according to WHO Directives, which clearly state that the ALP (alkaline phosphatase) substance in pasteurized milk must be totally inactivated, by implementing a newly developed method. The study, additionally, focused on repeatability, stability of results, the effect of the environmental temperature, the effect of the different origins of the milk and convenience with respect to performance characteristics of three methods for the detection of ALP. The milk samples were collected from different areas of Greece during February 2016–February 2018 and May 2019–January 2020. The novel enzymatic biochemical method, named the “AP test”, showed superior characteristics for a diversity of materials such as milk, whey, cheese and butter in comparison to the other two methods that were used for screening and quantitative estimation of the concentration of ALP in samples. |
| first_indexed | 2024-03-10T03:25:03Z |
| format | Article |
| id | doaj.art-10a3379d5e514a2b989dd86bc1cfabc5 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T03:25:03Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-10a3379d5e514a2b989dd86bc1cfabc52023-11-23T09:53:51ZengMDPI AGApplied Sciences2076-34172022-05-011210483310.3390/app12104833Assessment of Seasonal and Diurnal Variations of Alkaline Phosphatase Activity in Pasteurized MilkVaia Gerokomou0Georgios Rozos1Panagiotis Demertzis2Konstantoula Akrida-Demertzi3Food Chemistry Lab, Section of Industrial and Food Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceDepartment of Agriculture, School of Agriculture, University of Ioannina, Kostakioi Artas, 47100 Arta, GreeceFood Chemistry Lab, Section of Industrial and Food Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceFood Chemistry Lab, Section of Industrial and Food Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceThe present study was conducted to detect the concentration levels of ALP (alkaline phosphatase) in pasteurized milk and determine whether the pasteurization was successful or not, according to WHO Directives, which clearly state that the ALP (alkaline phosphatase) substance in pasteurized milk must be totally inactivated, by implementing a newly developed method. The study, additionally, focused on repeatability, stability of results, the effect of the environmental temperature, the effect of the different origins of the milk and convenience with respect to performance characteristics of three methods for the detection of ALP. The milk samples were collected from different areas of Greece during February 2016–February 2018 and May 2019–January 2020. The novel enzymatic biochemical method, named the “AP test”, showed superior characteristics for a diversity of materials such as milk, whey, cheese and butter in comparison to the other two methods that were used for screening and quantitative estimation of the concentration of ALP in samples.https://www.mdpi.com/2076-3417/12/10/4833food microbiologyfood industryalkaline phosphatasemilkmilk pasteurization |
| spellingShingle | Vaia Gerokomou Georgios Rozos Panagiotis Demertzis Konstantoula Akrida-Demertzi Assessment of Seasonal and Diurnal Variations of Alkaline Phosphatase Activity in Pasteurized Milk Applied Sciences food microbiology food industry alkaline phosphatase milk milk pasteurization |
| title | Assessment of Seasonal and Diurnal Variations of Alkaline Phosphatase Activity in Pasteurized Milk |
| title_full | Assessment of Seasonal and Diurnal Variations of Alkaline Phosphatase Activity in Pasteurized Milk |
| title_fullStr | Assessment of Seasonal and Diurnal Variations of Alkaline Phosphatase Activity in Pasteurized Milk |
| title_full_unstemmed | Assessment of Seasonal and Diurnal Variations of Alkaline Phosphatase Activity in Pasteurized Milk |
| title_short | Assessment of Seasonal and Diurnal Variations of Alkaline Phosphatase Activity in Pasteurized Milk |
| title_sort | assessment of seasonal and diurnal variations of alkaline phosphatase activity in pasteurized milk |
| topic | food microbiology food industry alkaline phosphatase milk milk pasteurization |
| url | https://www.mdpi.com/2076-3417/12/10/4833 |
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