An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene Explosives
In this study, we developed a surface plasmon resonance (SPR) sensor chip based on 2,4,6-trinitrotoluene (TNT) recognition peptide-modified single-walled carbon nanotubes (SWCNTs). The carboxylic acid-functionalized SWCNTs were immobilized on a 3-aminopropyltriethoxysilane (APTES)-modified SPR Au ch...
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| Format: | Article |
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MDPI AG
2018-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/18/12/4461 |
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| author | Jin Wang Sanyang Du Takeshi Onodera Rui Yatabe Masayoshi Tanaka Mina Okochi Kiyoshi Toko |
| author_facet | Jin Wang Sanyang Du Takeshi Onodera Rui Yatabe Masayoshi Tanaka Mina Okochi Kiyoshi Toko |
| author_sort | Jin Wang |
| collection | DOAJ |
| description | In this study, we developed a surface plasmon resonance (SPR) sensor chip based on 2,4,6-trinitrotoluene (TNT) recognition peptide-modified single-walled carbon nanotubes (SWCNTs). The carboxylic acid-functionalized SWCNTs were immobilized on a 3-aminopropyltriethoxysilane (APTES)-modified SPR Au chip surface. Through π-stacking between the aromatic amino acids and SWCNTs, the TNT recognition peptide TNTHCDR3 was immobilized onto the surface of the SWCNTs. The peptide⁻SWCNTs-modified sensor surface was confirmed and evaluated by atomic force microscope (AFM) observation. The peptide⁻SWCNTs hybrid SPR sensor chip exhibited enhanced sensitivity with a limit of detection (LOD) of 772 ppb and highly selective detection compared with commercialized carboxymethylated dextran matrix sensor chips. |
| first_indexed | 2024-04-11T12:15:44Z |
| format | Article |
| id | doaj.art-10a3e33e690f46cab0e44e9469808674 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T12:15:44Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-10a3e33e690f46cab0e44e94698086742022-12-22T04:24:19ZengMDPI AGSensors1424-82202018-12-011812446110.3390/s18124461s18124461An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene ExplosivesJin Wang0Sanyang Du1Takeshi Onodera2Rui Yatabe3Masayoshi Tanaka4Mina Okochi5Kiyoshi Toko6Research and Development Center for Five-Sense Devices, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395, JapanJST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, JapanJST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, JapanJST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, JapanJST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, JapanJST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, JapanResearch and Development Center for Five-Sense Devices, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395, JapanIn this study, we developed a surface plasmon resonance (SPR) sensor chip based on 2,4,6-trinitrotoluene (TNT) recognition peptide-modified single-walled carbon nanotubes (SWCNTs). The carboxylic acid-functionalized SWCNTs were immobilized on a 3-aminopropyltriethoxysilane (APTES)-modified SPR Au chip surface. Through π-stacking between the aromatic amino acids and SWCNTs, the TNT recognition peptide TNTHCDR3 was immobilized onto the surface of the SWCNTs. The peptide⁻SWCNTs-modified sensor surface was confirmed and evaluated by atomic force microscope (AFM) observation. The peptide⁻SWCNTs hybrid SPR sensor chip exhibited enhanced sensitivity with a limit of detection (LOD) of 772 ppb and highly selective detection compared with commercialized carboxymethylated dextran matrix sensor chips.https://www.mdpi.com/1424-8220/18/12/4461SPRTNTHCDR3SWCNTsπ-stackingTNT analogues |
| spellingShingle | Jin Wang Sanyang Du Takeshi Onodera Rui Yatabe Masayoshi Tanaka Mina Okochi Kiyoshi Toko An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene Explosives Sensors SPR TNTHCDR3 SWCNTs π-stacking TNT analogues |
| title | An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene Explosives |
| title_full | An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene Explosives |
| title_fullStr | An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene Explosives |
| title_full_unstemmed | An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene Explosives |
| title_short | An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene Explosives |
| title_sort | spr sensor chip based on peptide modified single walled carbon nanotubes with enhanced sensitivity and selectivity in the detection of 2 4 6 trinitrotoluene explosives |
| topic | SPR TNTHCDR3 SWCNTs π-stacking TNT analogues |
| url | https://www.mdpi.com/1424-8220/18/12/4461 |
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