Study on Activity of Cocoa Ethanolic Extract against Shigella dysenteriae
Shigella dysenteriae is a gastrointestinal pathogen which shows resistance to antibiotics. A study has been conducted to investigate alternative antibacterial agents, due to the emerging resistance of S. dysenteriae to ciprofloxacin and other antibiotic classes. In this study, antibacterial properti...
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| Format: | Article |
| Language: | English |
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Indonesian Coffee and Cocoa Research Institute
2016-08-01
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| Series: | Coffee and Cocoa Research Journal |
| Subjects: | |
| Online Access: | http://www.ccrjournal.com/index.php/ccrj/article/view/222 |
| _version_ | 1818757463261839360 |
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| author | Ariza Budi Tunjung-Sari Enny Suswati Diana Chusna Mufida Alfa Rianul Setiawan Rianul Setiawan Alvin Isnaini Mochamad Rizal Teguh Wahyudi Misnawi Jati |
| author_facet | Ariza Budi Tunjung-Sari Enny Suswati Diana Chusna Mufida Alfa Rianul Setiawan Rianul Setiawan Alvin Isnaini Mochamad Rizal Teguh Wahyudi Misnawi Jati |
| author_sort | Ariza Budi Tunjung-Sari |
| collection | DOAJ |
| description | Shigella dysenteriae is a gastrointestinal pathogen which shows resistance to antibiotics. A study has been conducted to investigate alternative antibacterial agents, due to the emerging resistance of S. dysenteriae to ciprofloxacin and other antibiotic classes. In this study, antibacterial properties of cocoa ethanolic extract (CEE) and its impact on growth and morphology of S. dysenteriae were evaluated. The effect of CEE on bacterial growth was assayed by using agar-well diffusion method and by observing morphological changes of bacterial cells through the use of scanning electron microscopy (SEM). Furthermore, CEE was also applied orally to mice infected with S. dysenteriae. The intestinal fluids was cultured in selective medium to evaluate growth of S. dysenteriae colonies. This study demonstrated that CEE at concentrations of 15.6 mg/mL inhibited S. dysenteriae growth, and at concentrations of 500 mg/mL and 1,000 mg/mL exhibited equal activity to 6.5 g/mL of ciprofloxacin. SEM showed that S. dysenteriae cells had formed filaments, indicating that CEE caused cellular stress to S. dysenteriae. In in vivo assay, CEE showed suppression of S. dysenteriae colony in the mice intestine. This research suggests that CEE could potentially be used as antibacterial agent againsts S. dysenteriae. |
| first_indexed | 2024-12-18T06:11:20Z |
| format | Article |
| id | doaj.art-10a4c0ebfa89421ca4da29b573857acb |
| institution | Directory Open Access Journal |
| issn | 0215-0212 2406-9574 |
| language | English |
| last_indexed | 2024-12-18T06:11:20Z |
| publishDate | 2016-08-01 |
| publisher | Indonesian Coffee and Cocoa Research Institute |
| record_format | Article |
| series | Coffee and Cocoa Research Journal |
| spelling | doaj.art-10a4c0ebfa89421ca4da29b573857acb2022-12-21T21:18:23ZengIndonesian Coffee and Cocoa Research InstituteCoffee and Cocoa Research Journal0215-02122406-95742016-08-0132210.22302/iccri.jur.pelitaperkebunan.v32i2.222222Study on Activity of Cocoa Ethanolic Extract against Shigella dysenteriaeAriza Budi Tunjung-Sari0Enny Suswati1Diana Chusna Mufida2Alfa Rianul Setiawan Rianul Setiawan3Alvin Isnaini4Mochamad Rizal5Teguh Wahyudi6Misnawi Jati7Indonesian Coffee and Cocoa Research InstituteFaculty of Medicine, Jember UniversityFaculty of Medicine, Jember UniversityFaculty of Medicine, Jember UniversityFaculty of Medicine, Jember UniversityFaculty of Medicine, Jember UniversityIndonesian Coffee and Cocoa Reseacrh InstituteIndonesian Coffee and Cocoa Reseacrh InstituteShigella dysenteriae is a gastrointestinal pathogen which shows resistance to antibiotics. A study has been conducted to investigate alternative antibacterial agents, due to the emerging resistance of S. dysenteriae to ciprofloxacin and other antibiotic classes. In this study, antibacterial properties of cocoa ethanolic extract (CEE) and its impact on growth and morphology of S. dysenteriae were evaluated. The effect of CEE on bacterial growth was assayed by using agar-well diffusion method and by observing morphological changes of bacterial cells through the use of scanning electron microscopy (SEM). Furthermore, CEE was also applied orally to mice infected with S. dysenteriae. The intestinal fluids was cultured in selective medium to evaluate growth of S. dysenteriae colonies. This study demonstrated that CEE at concentrations of 15.6 mg/mL inhibited S. dysenteriae growth, and at concentrations of 500 mg/mL and 1,000 mg/mL exhibited equal activity to 6.5 g/mL of ciprofloxacin. SEM showed that S. dysenteriae cells had formed filaments, indicating that CEE caused cellular stress to S. dysenteriae. In in vivo assay, CEE showed suppression of S. dysenteriae colony in the mice intestine. This research suggests that CEE could potentially be used as antibacterial agent againsts S. dysenteriae.http://www.ccrjournal.com/index.php/ccrj/article/view/222Antibacterialdysenterycocoaactive compoundantibiotic |
| spellingShingle | Ariza Budi Tunjung-Sari Enny Suswati Diana Chusna Mufida Alfa Rianul Setiawan Rianul Setiawan Alvin Isnaini Mochamad Rizal Teguh Wahyudi Misnawi Jati Study on Activity of Cocoa Ethanolic Extract against Shigella dysenteriae Coffee and Cocoa Research Journal Antibacterial dysentery cocoa active compound antibiotic |
| title | Study on Activity of Cocoa Ethanolic Extract against Shigella dysenteriae |
| title_full | Study on Activity of Cocoa Ethanolic Extract against Shigella dysenteriae |
| title_fullStr | Study on Activity of Cocoa Ethanolic Extract against Shigella dysenteriae |
| title_full_unstemmed | Study on Activity of Cocoa Ethanolic Extract against Shigella dysenteriae |
| title_short | Study on Activity of Cocoa Ethanolic Extract against Shigella dysenteriae |
| title_sort | study on activity of cocoa ethanolic extract against shigella dysenteriae |
| topic | Antibacterial dysentery cocoa active compound antibiotic |
| url | http://www.ccrjournal.com/index.php/ccrj/article/view/222 |
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