Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing
Abstract Patients with diabetes experience delayed wound healing because of the uncontrolled glucose level in their bloodstream, which leads to impaired function of white blood cells, poor circulation, decreased production and repair of new blood vessels. Treatment using polydeoxyribonucleotide (PDR...
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| Format: | Article |
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Nature Portfolio
2020-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-74004-0 |
| _version_ | 1818425463044833280 |
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| author | Da Yong Shin Ji-Ung Park Min-Ha Choi Sukwha Kim Hyoun-Ee Kim Seol-Ha Jeong |
| author_facet | Da Yong Shin Ji-Ung Park Min-Ha Choi Sukwha Kim Hyoun-Ee Kim Seol-Ha Jeong |
| author_sort | Da Yong Shin |
| collection | DOAJ |
| description | Abstract Patients with diabetes experience delayed wound healing because of the uncontrolled glucose level in their bloodstream, which leads to impaired function of white blood cells, poor circulation, decreased production and repair of new blood vessels. Treatment using polydeoxyribonucleotide (PDRN), which is a DNA extracted from the sperm cells of salmon, has been introduced to accelerate the healing process of diabetic wounds. To accelerate the wound-healing process, sustained delivery of PDRN is critical. In this study, taking advantage of the non-invasive gelation property of alginate, PDRN was loaded inside the hydrogel (Alg-PDRN). The release behavior of PDRN was altered by controlling the crosslinking density of the Alg hydrogel. The amount of PDRN was the greatest inside the hydrogel with the highest crosslinking density because of the decreased diffusion. However, there was an optimal degree of crosslinking for the effective release of PDRN. In vitro studies using human dermal fibroblasts and diabetes mellitus fibroblasts and an in ovo chorioallantoic membrane assay confirmed that the Alg-PDRN hydrogel effectively induced cell proliferation and expression of angiogenic growth factors and promoted new blood vessel formation. Its effectiveness for accelerated diabetic wound healing was also confirmed in an in-vivo animal experiment using a diabetic mouse model. |
| first_indexed | 2024-12-14T14:14:20Z |
| format | Article |
| id | doaj.art-10a620102bcc46ecbf25c229e255736d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T14:14:20Z |
| publishDate | 2020-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-10a620102bcc46ecbf25c229e255736d2022-12-21T22:58:13ZengNature PortfolioScientific Reports2045-23222020-10-0110111410.1038/s41598-020-74004-0Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healingDa Yong Shin0Ji-Ung Park1Min-Ha Choi2Sukwha Kim3Hyoun-Ee Kim4Seol-Ha Jeong5Department of Materials Science and Engineering, Seoul National UniversityDepartment of Plastic and Reconstructive Surgery, Seoul National University Boramae Medical CenterDepartment of Plastic and Reconstructive Surgery, Seoul National University Boramae Medical CenterMedical Big Data Research Center, Seoul National University College of MedicineDepartment of Materials Science and Engineering, Seoul National UniversityDepartment of Materials Science and Engineering, Seoul National UniversityAbstract Patients with diabetes experience delayed wound healing because of the uncontrolled glucose level in their bloodstream, which leads to impaired function of white blood cells, poor circulation, decreased production and repair of new blood vessels. Treatment using polydeoxyribonucleotide (PDRN), which is a DNA extracted from the sperm cells of salmon, has been introduced to accelerate the healing process of diabetic wounds. To accelerate the wound-healing process, sustained delivery of PDRN is critical. In this study, taking advantage of the non-invasive gelation property of alginate, PDRN was loaded inside the hydrogel (Alg-PDRN). The release behavior of PDRN was altered by controlling the crosslinking density of the Alg hydrogel. The amount of PDRN was the greatest inside the hydrogel with the highest crosslinking density because of the decreased diffusion. However, there was an optimal degree of crosslinking for the effective release of PDRN. In vitro studies using human dermal fibroblasts and diabetes mellitus fibroblasts and an in ovo chorioallantoic membrane assay confirmed that the Alg-PDRN hydrogel effectively induced cell proliferation and expression of angiogenic growth factors and promoted new blood vessel formation. Its effectiveness for accelerated diabetic wound healing was also confirmed in an in-vivo animal experiment using a diabetic mouse model.https://doi.org/10.1038/s41598-020-74004-0 |
| spellingShingle | Da Yong Shin Ji-Ung Park Min-Ha Choi Sukwha Kim Hyoun-Ee Kim Seol-Ha Jeong Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing Scientific Reports |
| title | Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing |
| title_full | Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing |
| title_fullStr | Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing |
| title_full_unstemmed | Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing |
| title_short | Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing |
| title_sort | polydeoxyribonucleotide delivering therapeutic hydrogel for diabetic wound healing |
| url | https://doi.org/10.1038/s41598-020-74004-0 |
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