Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells
Li et al. report that co-delivering a dominant negative fragment of p53 (p53DD) greatly enhances precise editing efficiencies for prime editing and cytosine base editing in human pluripotent stem cells, without compromising the genome-wide safety.
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-34045-7 |
| _version_ | 1811257179237253120 |
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| author | Mu Li Aaron Zhong Youjun Wu Mega Sidharta Michael Beaury Xiaolan Zhao Lorenz Studer Ting Zhou |
| author_facet | Mu Li Aaron Zhong Youjun Wu Mega Sidharta Michael Beaury Xiaolan Zhao Lorenz Studer Ting Zhou |
| author_sort | Mu Li |
| collection | DOAJ |
| description | Li et al. report that co-delivering a dominant negative fragment of p53 (p53DD) greatly enhances precise editing efficiencies for prime editing and cytosine base editing in human pluripotent stem cells, without compromising the genome-wide safety. |
| first_indexed | 2024-04-12T17:53:23Z |
| format | Article |
| id | doaj.art-10a894ea600a4c1c8a1340e972626ca9 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T17:53:23Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-10a894ea600a4c1c8a1340e972626ca92022-12-22T03:22:26ZengNature PortfolioNature Communications2041-17232022-10-0113111210.1038/s41467-022-34045-7Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cellsMu Li0Aaron Zhong1Youjun Wu2Mega Sidharta3Michael Beaury4Xiaolan Zhao5Lorenz Studer6Ting Zhou7The SKI Stem Cell Research Facility, The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer ResearchThe SKI Stem Cell Research Facility, The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer ResearchThe SKI Stem Cell Research Facility, The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer ResearchThe SKI Stem Cell Research Facility, The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer ResearchThe SKI Stem Cell Research Facility, The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer ResearchMolecular Biology Program, Memorial Sloan Kettering Cancer CenterThe Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer ResearchThe SKI Stem Cell Research Facility, The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer ResearchLi et al. report that co-delivering a dominant negative fragment of p53 (p53DD) greatly enhances precise editing efficiencies for prime editing and cytosine base editing in human pluripotent stem cells, without compromising the genome-wide safety.https://doi.org/10.1038/s41467-022-34045-7 |
| spellingShingle | Mu Li Aaron Zhong Youjun Wu Mega Sidharta Michael Beaury Xiaolan Zhao Lorenz Studer Ting Zhou Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells Nature Communications |
| title | Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells |
| title_full | Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells |
| title_fullStr | Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells |
| title_full_unstemmed | Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells |
| title_short | Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells |
| title_sort | transient inhibition of p53 enhances prime editing and cytosine base editing efficiencies in human pluripotent stem cells |
| url | https://doi.org/10.1038/s41467-022-34045-7 |
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