Iodoacetate and allogenous cartilage particles as models for arthritis induction in equine
Experimental models of osteoarthritis (OA) have been widely developed in different animal species, because of the high incidence of osteoarthritis diseases in humans and animals. To date, no ideal OA animal model has been reported. The present study compare different osteoarthritis models to determi...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2014-12-01
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| Series: | International Journal of Veterinary Science and Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2314459914000301 |
| _version_ | 1818296304481075200 |
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| author | Ahmed Elmesiry Magdi Seleim David Cullis-Hill |
| author_facet | Ahmed Elmesiry Magdi Seleim David Cullis-Hill |
| author_sort | Ahmed Elmesiry |
| collection | DOAJ |
| description | Experimental models of osteoarthritis (OA) have been widely developed in different animal species, because of the high incidence of osteoarthritis diseases in humans and animals. To date, no ideal OA animal model has been reported. The present study compare different osteoarthritis models to determine which one is suitable for inducing experimental equine OA. Fifteen donkeys were divided into three equal groups (n = 5). The radio carpal joints of the right forelimb of 15 donkeys were injected with 25 mg monoiodoacetate (MIA) (group A), 50 mg allogenous cartilage particles (ACP) (group B), or vehicle solution (group C) over a period of 70 days. Osteoarthritis induction was evaluated weekly through lameness score, carpal circumference, joint flexion angel, synovial fluid analysis (total protein and WBC count), and radiology. Animal were euthanized and joints histopathology were performed at 70 days. Lameness score and joint circumference was increased in both group A and B however joint flexion angel was decreased compared to group C (p < 0.05). Osteophytes were observed in MIA injected joints only accompanied with subchondral bone sclerosis. Cartilage damage was observed grossly and histologically in Group A together with synovial membrane fibrosis. Group B had on cartilage damage grossly however histological examination revealed some cartilage surface discontinuity with synovial membrane edema. Injection of monoiodoacetate in the donkey is a successful model to create the acute clinical signs of joint disease as well as cartilage damage. However, allogenous cartilage particles injection need more investigation to be applied. |
| first_indexed | 2024-12-13T04:01:25Z |
| format | Article |
| id | doaj.art-10aa2bc206c2487aad87486d4760374c |
| institution | Directory Open Access Journal |
| issn | 2314-4599 |
| language | English |
| last_indexed | 2024-12-13T04:01:25Z |
| publishDate | 2014-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Veterinary Science and Medicine |
| spelling | doaj.art-10aa2bc206c2487aad87486d4760374c2022-12-22T00:00:26ZengTaylor & Francis GroupInternational Journal of Veterinary Science and Medicine2314-45992014-12-012214215010.1016/j.ijvsm.2014.11.001Iodoacetate and allogenous cartilage particles as models for arthritis induction in equineAhmed Elmesiry0Magdi Seleim1David Cullis-Hill2Animal Reproduction Research Institute, Agriculture Research Center, 12556 Haram, Giza, EgyptDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33156 Kafrelsheikh, EgyptBiopharm Pharmaceuticals, 111 Bronte Rd, Bondi Junction, Sydney, 2022 NSW, AustraliaExperimental models of osteoarthritis (OA) have been widely developed in different animal species, because of the high incidence of osteoarthritis diseases in humans and animals. To date, no ideal OA animal model has been reported. The present study compare different osteoarthritis models to determine which one is suitable for inducing experimental equine OA. Fifteen donkeys were divided into three equal groups (n = 5). The radio carpal joints of the right forelimb of 15 donkeys were injected with 25 mg monoiodoacetate (MIA) (group A), 50 mg allogenous cartilage particles (ACP) (group B), or vehicle solution (group C) over a period of 70 days. Osteoarthritis induction was evaluated weekly through lameness score, carpal circumference, joint flexion angel, synovial fluid analysis (total protein and WBC count), and radiology. Animal were euthanized and joints histopathology were performed at 70 days. Lameness score and joint circumference was increased in both group A and B however joint flexion angel was decreased compared to group C (p < 0.05). Osteophytes were observed in MIA injected joints only accompanied with subchondral bone sclerosis. Cartilage damage was observed grossly and histologically in Group A together with synovial membrane fibrosis. Group B had on cartilage damage grossly however histological examination revealed some cartilage surface discontinuity with synovial membrane edema. Injection of monoiodoacetate in the donkey is a successful model to create the acute clinical signs of joint disease as well as cartilage damage. However, allogenous cartilage particles injection need more investigation to be applied.http://www.sciencedirect.com/science/article/pii/S2314459914000301EquineLamenessJointCartilageOsteoarthritis |
| spellingShingle | Ahmed Elmesiry Magdi Seleim David Cullis-Hill Iodoacetate and allogenous cartilage particles as models for arthritis induction in equine International Journal of Veterinary Science and Medicine Equine Lameness Joint Cartilage Osteoarthritis |
| title | Iodoacetate and allogenous cartilage particles as models for arthritis induction in equine |
| title_full | Iodoacetate and allogenous cartilage particles as models for arthritis induction in equine |
| title_fullStr | Iodoacetate and allogenous cartilage particles as models for arthritis induction in equine |
| title_full_unstemmed | Iodoacetate and allogenous cartilage particles as models for arthritis induction in equine |
| title_short | Iodoacetate and allogenous cartilage particles as models for arthritis induction in equine |
| title_sort | iodoacetate and allogenous cartilage particles as models for arthritis induction in equine |
| topic | Equine Lameness Joint Cartilage Osteoarthritis |
| url | http://www.sciencedirect.com/science/article/pii/S2314459914000301 |
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