Quantitation and integrity evaluation of RNA genome in lentiviral vectors by direct reverse transcription-droplet digital PCR (direct RT-ddPCR)
Abstract Lentiviral vectors (LV) have proven to be powerful tools for stable gene delivery in both dividing and non-dividing cells. Approval of these LVs for use in clinical applications has been achieved by improvements in LV design. Critically important characteristics concerning quality control a...
Main Authors: | Zhiyong He, Edward J. Kwee, Megan H. Cleveland, Kenneth D. Cole, Sheng Lin-Gibson, Hua-Jun He |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2023-09-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-023-41644-x |
Similar Items
-
Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)
by: Samuel Long, et al.
Published: (2021-02-01) -
Maximizing viral detection with SIV droplet digital PCR (ddPCR) assays.
by: Samuel Long, et al.
Published: (2020-01-01) -
Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
by: Changwoo Park, et al.
Published: (2023-02-01) -
Droplet Digital PCR (ddPCR) Analysis for the Detection and Quantification of Cow DNA in Buffalo Mozzarella Cheese
by: Anna Cutarelli, et al.
Published: (2021-04-01) -
Development and optimization of a simian immunodeficiency virus (SIV) droplet digital PCR (ddPCR) assay.
by: Samuel Long, et al.
Published: (2020-01-01)