Seasonal Shifts in Cold Tolerance and the Composition of the Gut Microbiome of <i>Dendroctonus valens</i> LeConte Occur Concurrently
<i>Dendroctonus valens</i> LeConte, an invasive bark beetle, has caused severe damage in pine forests and has the potential to disperse into new geographic ranges in China. Although the gut microbiota of <i>D. valens</i> and its fundamental role in host fitness have been inve...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/12/7/888 |
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| author | Zehai Hou Yaxin Dong Fengming Shi Yabei Xu Sixun Ge Jing Tao Lili Ren Shixiang Zong |
| author_facet | Zehai Hou Yaxin Dong Fengming Shi Yabei Xu Sixun Ge Jing Tao Lili Ren Shixiang Zong |
| author_sort | Zehai Hou |
| collection | DOAJ |
| description | <i>Dendroctonus valens</i> LeConte, an invasive bark beetle, has caused severe damage in pine forests and has the potential to disperse into new geographic ranges in China. Although the gut microbiota of <i>D. valens</i> and its fundamental role in host fitness have been investigated widely, little is known about the relationship between the seasonal shifts of both cold tolerance and the gut microbiome of <i>D. valens</i> during overwintering, which occurs at the larval stage. In this study, to examine seasonal variations in the composition of the microbiome, we collected <i>D. valens</i> larvae in September (autumn), January (winter), and May (spring), and then analyzed the bacterial and fungal communities of the gut via sequencing of partial 16S rRNA and ITS genes. In addition, changes in the supercooling capacity and antioxidant enzyme activities of <i>D. valens</i> larvae collected in the different seasons were evaluated. Overwintering resulted in changes to microbial communities. In particular, the abundances of <i>Enterobacter</i>, <i>Serratia</i>, <i>Erwinia</i>, and <i>Klebsiella</i> decreased during overwintering. Concurrent with these changes, the cold tolerance of <i>D. valens</i> larvae was enhanced during overwintering, and the activities of the antioxidant enzymes catalase and peroxidase were reduced. We hypothesize that seasonal shifts in the gut microbiome may be connected to changes in cold tolerance and antioxidant enzyme activity in <i>D. valens</i>. It will be worthwhile to confirm whether seasonal changes in the microbiome contribute to the success of host overwintering. |
| first_indexed | 2024-03-10T09:38:55Z |
| format | Article |
| id | doaj.art-10b8bd6a22d04f00b00c907a45b78d02 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T09:38:55Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-10b8bd6a22d04f00b00c907a45b78d022023-11-22T03:49:12ZengMDPI AGForests1999-49072021-07-0112788810.3390/f12070888Seasonal Shifts in Cold Tolerance and the Composition of the Gut Microbiome of <i>Dendroctonus valens</i> LeConte Occur ConcurrentlyZehai Hou0Yaxin Dong1Fengming Shi2Yabei Xu3Sixun Ge4Jing Tao5Lili Ren6Shixiang Zong7Key Laboratory of Beijing for the Control of Forest Pests, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Beijing for the Control of Forest Pests, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Beijing for the Control of Forest Pests, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Beijing for the Control of Forest Pests, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Beijing for the Control of Forest Pests, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Beijing for the Control of Forest Pests, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Beijing for the Control of Forest Pests, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Beijing for the Control of Forest Pests, Beijing Forestry University, Beijing 100083, China<i>Dendroctonus valens</i> LeConte, an invasive bark beetle, has caused severe damage in pine forests and has the potential to disperse into new geographic ranges in China. Although the gut microbiota of <i>D. valens</i> and its fundamental role in host fitness have been investigated widely, little is known about the relationship between the seasonal shifts of both cold tolerance and the gut microbiome of <i>D. valens</i> during overwintering, which occurs at the larval stage. In this study, to examine seasonal variations in the composition of the microbiome, we collected <i>D. valens</i> larvae in September (autumn), January (winter), and May (spring), and then analyzed the bacterial and fungal communities of the gut via sequencing of partial 16S rRNA and ITS genes. In addition, changes in the supercooling capacity and antioxidant enzyme activities of <i>D. valens</i> larvae collected in the different seasons were evaluated. Overwintering resulted in changes to microbial communities. In particular, the abundances of <i>Enterobacter</i>, <i>Serratia</i>, <i>Erwinia</i>, and <i>Klebsiella</i> decreased during overwintering. Concurrent with these changes, the cold tolerance of <i>D. valens</i> larvae was enhanced during overwintering, and the activities of the antioxidant enzymes catalase and peroxidase were reduced. We hypothesize that seasonal shifts in the gut microbiome may be connected to changes in cold tolerance and antioxidant enzyme activity in <i>D. valens</i>. It will be worthwhile to confirm whether seasonal changes in the microbiome contribute to the success of host overwintering.https://www.mdpi.com/1999-4907/12/7/888antioxidant enzymecold-hardinessoverwinteringred turpentine beetlesupercooling pointsymbiosis |
| spellingShingle | Zehai Hou Yaxin Dong Fengming Shi Yabei Xu Sixun Ge Jing Tao Lili Ren Shixiang Zong Seasonal Shifts in Cold Tolerance and the Composition of the Gut Microbiome of <i>Dendroctonus valens</i> LeConte Occur Concurrently Forests antioxidant enzyme cold-hardiness overwintering red turpentine beetle supercooling point symbiosis |
| title | Seasonal Shifts in Cold Tolerance and the Composition of the Gut Microbiome of <i>Dendroctonus valens</i> LeConte Occur Concurrently |
| title_full | Seasonal Shifts in Cold Tolerance and the Composition of the Gut Microbiome of <i>Dendroctonus valens</i> LeConte Occur Concurrently |
| title_fullStr | Seasonal Shifts in Cold Tolerance and the Composition of the Gut Microbiome of <i>Dendroctonus valens</i> LeConte Occur Concurrently |
| title_full_unstemmed | Seasonal Shifts in Cold Tolerance and the Composition of the Gut Microbiome of <i>Dendroctonus valens</i> LeConte Occur Concurrently |
| title_short | Seasonal Shifts in Cold Tolerance and the Composition of the Gut Microbiome of <i>Dendroctonus valens</i> LeConte Occur Concurrently |
| title_sort | seasonal shifts in cold tolerance and the composition of the gut microbiome of i dendroctonus valens i leconte occur concurrently |
| topic | antioxidant enzyme cold-hardiness overwintering red turpentine beetle supercooling point symbiosis |
| url | https://www.mdpi.com/1999-4907/12/7/888 |
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