Phototoxicity activity of Psoralea drupacea L. using Atremia salina bioassay system
Objective: Phototoxicity is a kind of dermatitis that is activated by exposure to ultraviolet light following the administration of some drugs or natural products. Artemia salina (A. salina) (brine shrimp) has been effectively applied for toxicity testing and is perfect for biological screening of m...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2011-07-01
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| Series: | Avicenna Journal of Phytomedicine |
| Subjects: | |
| Online Access: | http://ajp.mums.ac.ir/pdf_117_65d4840cac5e8facb61a07be24ea17ac.html |
| _version_ | 1817990154185342976 |
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| author | Mohammad Ramezani Hossein Hosseinzadeh M. Moradi E. Taghiabadi |
| author_facet | Mohammad Ramezani Hossein Hosseinzadeh M. Moradi E. Taghiabadi |
| author_sort | Mohammad Ramezani |
| collection | DOAJ |
| description | Objective: Phototoxicity is a kind of dermatitis that is activated by exposure to ultraviolet light following the administration of some drugs or natural products. Artemia salina (A. salina) (brine shrimp) has been effectively applied for toxicity testing and is perfect for biological screening of many chemicals for simultaneous evaluation of toxicity and phototoxicity. The objective of this study was to investigate the phototoxic activitiy of the methanolic extract and chloroform and CH3OH/H2O2 fraction of Psoralea drupacea (P. drupacea).
Materials and methods: The phototoxic effect of the methanolic extract, chloroform and CH3OH/H2O2 fractions of P. drupacea was evaluatedusing A. salina bioassay system. Different concentrations of methanolic extract and fractions of P. drupacea were added to the plate of one-day old larvae followed by exposure to UV radiation at 366 nm in three different exposure times (0, 4 and 20 h). Mortality was determined 24h after the start of the irradiation.
Results: The value of LC50 of P. drupacea methanolic extract and methoxalen as positive control were 0.64 and 3.5´10-4 mg/ml, respectively. P. drupacea methanolic extract and chloroform fraction demonstrated phototoxic activity after 4 h radiation.
Conclusion: The result showed that P. drupacea methanolic extract and chloroform fraction have phototoxicity in A. salina bioassay system and their toxic effect is related to phototoxic constituents such as psoralen. |
| first_indexed | 2024-04-14T00:55:08Z |
| format | Article |
| id | doaj.art-10bd64b8599d4ad187f3a6cdf1699ca2 |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-04-14T00:55:08Z |
| publishDate | 2011-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-10bd64b8599d4ad187f3a6cdf1699ca22022-12-22T02:21:38ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492011-07-01112428117Phototoxicity activity of Psoralea drupacea L. using Atremia salina bioassay systemMohammad Ramezani0Hossein Hosseinzadeh1M. Moradi2E. Taghiabadi3Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. IranPharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. IranDepartment of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical sciences, Mashhad, I. R. IranDepartment of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical sciences, Mashhad, I. R. IranObjective: Phototoxicity is a kind of dermatitis that is activated by exposure to ultraviolet light following the administration of some drugs or natural products. Artemia salina (A. salina) (brine shrimp) has been effectively applied for toxicity testing and is perfect for biological screening of many chemicals for simultaneous evaluation of toxicity and phototoxicity. The objective of this study was to investigate the phototoxic activitiy of the methanolic extract and chloroform and CH3OH/H2O2 fraction of Psoralea drupacea (P. drupacea). Materials and methods: The phototoxic effect of the methanolic extract, chloroform and CH3OH/H2O2 fractions of P. drupacea was evaluatedusing A. salina bioassay system. Different concentrations of methanolic extract and fractions of P. drupacea were added to the plate of one-day old larvae followed by exposure to UV radiation at 366 nm in three different exposure times (0, 4 and 20 h). Mortality was determined 24h after the start of the irradiation. Results: The value of LC50 of P. drupacea methanolic extract and methoxalen as positive control were 0.64 and 3.5´10-4 mg/ml, respectively. P. drupacea methanolic extract and chloroform fraction demonstrated phototoxic activity after 4 h radiation. Conclusion: The result showed that P. drupacea methanolic extract and chloroform fraction have phototoxicity in A. salina bioassay system and their toxic effect is related to phototoxic constituents such as psoralen.http://ajp.mums.ac.ir/pdf_117_65d4840cac5e8facb61a07be24ea17ac.htmlArtemia salinaPsoralea drupaceaPhototoxicityToxicity |
| spellingShingle | Mohammad Ramezani Hossein Hosseinzadeh M. Moradi E. Taghiabadi Phototoxicity activity of Psoralea drupacea L. using Atremia salina bioassay system Avicenna Journal of Phytomedicine Artemia salina Psoralea drupacea Phototoxicity Toxicity |
| title | Phototoxicity activity of Psoralea drupacea L. using Atremia salina bioassay system |
| title_full | Phototoxicity activity of Psoralea drupacea L. using Atremia salina bioassay system |
| title_fullStr | Phototoxicity activity of Psoralea drupacea L. using Atremia salina bioassay system |
| title_full_unstemmed | Phototoxicity activity of Psoralea drupacea L. using Atremia salina bioassay system |
| title_short | Phototoxicity activity of Psoralea drupacea L. using Atremia salina bioassay system |
| title_sort | phototoxicity activity of psoralea drupacea l using atremia salina bioassay system |
| topic | Artemia salina Psoralea drupacea Phototoxicity Toxicity |
| url | http://ajp.mums.ac.ir/pdf_117_65d4840cac5e8facb61a07be24ea17ac.html |
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