Anti-tumor effect of bisphosphonate (YM529) on non-small cell lung cancer cell lines
<p>Abstract</p> <p>Background</p> <p>YM529 is a newly developed nitrogen-containing bisphosphonate (BP) classified as a third-generation BP that shows a 100-fold greater potency against bone resorption than pamidronate, a second-generation BP. This agent is, therefore e...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-01-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/7/8 |
| _version_ | 1819140084868317184 |
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| author | Date Hiroshi Sano Yoshifumi Tokumo Masaki Ouchida Mamoru Hara Fumikata Toyooka Shinichi Aoe Motoi Koshimune Ryuichiro Shimizu Nobuyoshi |
| author_facet | Date Hiroshi Sano Yoshifumi Tokumo Masaki Ouchida Mamoru Hara Fumikata Toyooka Shinichi Aoe Motoi Koshimune Ryuichiro Shimizu Nobuyoshi |
| author_sort | Date Hiroshi |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>YM529 is a newly developed nitrogen-containing bisphosphonate (BP) classified as a third-generation BP that shows a 100-fold greater potency against bone resorption than pamidronate, a second-generation BP. This agent is, therefore expected to be extremely useful clinically for the treatment of osteoporosis and hypercalcemia. Recently, YM529 as well as other third-generation BPs have also been shown to exert anti-tumor effects against various types of cancer cells both <it>in vitro </it>or/and <it>in vivo</it>. In this study, we investigate the anti-tumor effect of YM529 on non-small cell lung cancer (NSCLC).</p> <p>Methods</p> <p>Direct anti-tumor effect of YM529 against 8 NSCLC cell lines (adenocarcinoma: H23, H1299, NCI-H1819, NCI-H2009, H44, A549, adenosquamous cell carcinoma: NCI-H125, squamous cell carcinoma: NCI-H157) were measured by MTS assay and calculated inhibition concentration 50 % (IC<sub>50</sub>) values. YM529 induced apoptosis of NCI-H1819 was examined by DNA fragmentation of 2 % agarose gel electrophoresis and flowcytometric analysis (sub-G<sub>1 </sub>method). We examined where YM529 given effect to apoptosis of NSCLC cells in signaling pathway of the mevalonate pathway by western blotting analysis.</p> <p>Results</p> <p>We found that there was direct anti-tumor effect of YM529 on 8 NSCLC cell lines in a dose-dependent manner and their IC<sub>50 </sub>values were 2.1 to 7.9 μM and YM529 induced apoptosis and G<sub>1 </sub>arrest cell cycle with dose-dependent manner and YM529 caused down regulation of phospholyration of ERK1/2 in signaling pathways of NSCLC cell line (NCI-H1819).</p> <p>Conclusion</p> <p>Our study demonstrate that YM529 showed direct anti-tumor effect on NSCLC cell lines in vitro, which supports the possibility that third-generation BPs including YM529 can be one of therapeutic options for NSCLC.</p> |
| first_indexed | 2024-12-22T11:32:56Z |
| format | Article |
| id | doaj.art-10c0e4dd9c5a4bbcbe4906d485e1fd30 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-22T11:32:56Z |
| publishDate | 2007-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-10c0e4dd9c5a4bbcbe4906d485e1fd302022-12-21T18:27:33ZengBMCBMC Cancer1471-24072007-01-0171810.1186/1471-2407-7-8Anti-tumor effect of bisphosphonate (YM529) on non-small cell lung cancer cell linesDate HiroshiSano YoshifumiTokumo MasakiOuchida MamoruHara FumikataToyooka ShinichiAoe MotoiKoshimune RyuichiroShimizu Nobuyoshi<p>Abstract</p> <p>Background</p> <p>YM529 is a newly developed nitrogen-containing bisphosphonate (BP) classified as a third-generation BP that shows a 100-fold greater potency against bone resorption than pamidronate, a second-generation BP. This agent is, therefore expected to be extremely useful clinically for the treatment of osteoporosis and hypercalcemia. Recently, YM529 as well as other third-generation BPs have also been shown to exert anti-tumor effects against various types of cancer cells both <it>in vitro </it>or/and <it>in vivo</it>. In this study, we investigate the anti-tumor effect of YM529 on non-small cell lung cancer (NSCLC).</p> <p>Methods</p> <p>Direct anti-tumor effect of YM529 against 8 NSCLC cell lines (adenocarcinoma: H23, H1299, NCI-H1819, NCI-H2009, H44, A549, adenosquamous cell carcinoma: NCI-H125, squamous cell carcinoma: NCI-H157) were measured by MTS assay and calculated inhibition concentration 50 % (IC<sub>50</sub>) values. YM529 induced apoptosis of NCI-H1819 was examined by DNA fragmentation of 2 % agarose gel electrophoresis and flowcytometric analysis (sub-G<sub>1 </sub>method). We examined where YM529 given effect to apoptosis of NSCLC cells in signaling pathway of the mevalonate pathway by western blotting analysis.</p> <p>Results</p> <p>We found that there was direct anti-tumor effect of YM529 on 8 NSCLC cell lines in a dose-dependent manner and their IC<sub>50 </sub>values were 2.1 to 7.9 μM and YM529 induced apoptosis and G<sub>1 </sub>arrest cell cycle with dose-dependent manner and YM529 caused down regulation of phospholyration of ERK1/2 in signaling pathways of NSCLC cell line (NCI-H1819).</p> <p>Conclusion</p> <p>Our study demonstrate that YM529 showed direct anti-tumor effect on NSCLC cell lines in vitro, which supports the possibility that third-generation BPs including YM529 can be one of therapeutic options for NSCLC.</p>http://www.biomedcentral.com/1471-2407/7/8 |
| spellingShingle | Date Hiroshi Sano Yoshifumi Tokumo Masaki Ouchida Mamoru Hara Fumikata Toyooka Shinichi Aoe Motoi Koshimune Ryuichiro Shimizu Nobuyoshi Anti-tumor effect of bisphosphonate (YM529) on non-small cell lung cancer cell lines BMC Cancer |
| title | Anti-tumor effect of bisphosphonate (YM529) on non-small cell lung cancer cell lines |
| title_full | Anti-tumor effect of bisphosphonate (YM529) on non-small cell lung cancer cell lines |
| title_fullStr | Anti-tumor effect of bisphosphonate (YM529) on non-small cell lung cancer cell lines |
| title_full_unstemmed | Anti-tumor effect of bisphosphonate (YM529) on non-small cell lung cancer cell lines |
| title_short | Anti-tumor effect of bisphosphonate (YM529) on non-small cell lung cancer cell lines |
| title_sort | anti tumor effect of bisphosphonate ym529 on non small cell lung cancer cell lines |
| url | http://www.biomedcentral.com/1471-2407/7/8 |
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