Phylogeography, salinity adaptations and metabolic potential of the Candidate Division KB1 Bacteria based on a partial single cell genome.
Deep-sea hypersaline anoxic basins (DHABs) and other hypersaline environments contain abundant and diverse microbial life that has adapted to these extreme conditions. The bacterial Candidate Division KB1 represents one of several uncultured groups that has been consistently observed in hypersaline...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-08-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01266/full |
| _version_ | 1818307383276863488 |
|---|---|
| author | Lisa M Nigro Andrew S Hyde Barbara J. MacGregor Andreas Teske |
| author_facet | Lisa M Nigro Andrew S Hyde Barbara J. MacGregor Andreas Teske |
| author_sort | Lisa M Nigro |
| collection | DOAJ |
| description | Deep-sea hypersaline anoxic basins (DHABs) and other hypersaline environments contain abundant and diverse microbial life that has adapted to these extreme conditions. The bacterial Candidate Division KB1 represents one of several uncultured groups that has been consistently observed in hypersaline microbial diversity studies. Here we report the phylogeography of KB1, its phylogenetic relationships to Candidate Division OP1 Bacteria, and its potential metabolic and osmotic stress adaptations based on a partial single cell amplified genome (SAG) of KB1 from Orca Basin, the largest hypersaline seafloor brine basin in the Gulf of Mexico. Our results are consistent with the hypothesis – previously developed based on 14C incorporation experiments with mixed-species enrichments from Mediterranean seafloor brines - that KB1 has adapted its proteins to elevated intracellular salinity, but at the same time KB1 apparently imports glycine betaine; this compatible solute is potentially not limited to osmoregulation but could also serve as a carbon and energy source. |
| first_indexed | 2024-12-13T06:57:30Z |
| format | Article |
| id | doaj.art-10c18617547549e9963c0d3336514a4a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T06:57:30Z |
| publishDate | 2016-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-10c18617547549e9963c0d3336514a4a2022-12-21T23:56:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-08-01710.3389/fmicb.2016.01266212635Phylogeography, salinity adaptations and metabolic potential of the Candidate Division KB1 Bacteria based on a partial single cell genome.Lisa M Nigro0Andrew S Hyde1Barbara J. MacGregor2Andreas Teske3University of North Carolina-Chapel HillUniversity of North Carolina-Chapel HillUniversity of North Carolina-Chapel HillUniversity of North Carolina-Chapel HillDeep-sea hypersaline anoxic basins (DHABs) and other hypersaline environments contain abundant and diverse microbial life that has adapted to these extreme conditions. The bacterial Candidate Division KB1 represents one of several uncultured groups that has been consistently observed in hypersaline microbial diversity studies. Here we report the phylogeography of KB1, its phylogenetic relationships to Candidate Division OP1 Bacteria, and its potential metabolic and osmotic stress adaptations based on a partial single cell amplified genome (SAG) of KB1 from Orca Basin, the largest hypersaline seafloor brine basin in the Gulf of Mexico. Our results are consistent with the hypothesis – previously developed based on 14C incorporation experiments with mixed-species enrichments from Mediterranean seafloor brines - that KB1 has adapted its proteins to elevated intracellular salinity, but at the same time KB1 apparently imports glycine betaine; this compatible solute is potentially not limited to osmoregulation but could also serve as a carbon and energy source.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01266/fullGenomePhylogeographyhypersalineOrca BasinCandidate Division KB1 |
| spellingShingle | Lisa M Nigro Andrew S Hyde Barbara J. MacGregor Andreas Teske Phylogeography, salinity adaptations and metabolic potential of the Candidate Division KB1 Bacteria based on a partial single cell genome. Frontiers in Microbiology Genome Phylogeography hypersaline Orca Basin Candidate Division KB1 |
| title | Phylogeography, salinity adaptations and metabolic potential of the Candidate Division KB1 Bacteria based on a partial single cell genome. |
| title_full | Phylogeography, salinity adaptations and metabolic potential of the Candidate Division KB1 Bacteria based on a partial single cell genome. |
| title_fullStr | Phylogeography, salinity adaptations and metabolic potential of the Candidate Division KB1 Bacteria based on a partial single cell genome. |
| title_full_unstemmed | Phylogeography, salinity adaptations and metabolic potential of the Candidate Division KB1 Bacteria based on a partial single cell genome. |
| title_short | Phylogeography, salinity adaptations and metabolic potential of the Candidate Division KB1 Bacteria based on a partial single cell genome. |
| title_sort | phylogeography salinity adaptations and metabolic potential of the candidate division kb1 bacteria based on a partial single cell genome |
| topic | Genome Phylogeography hypersaline Orca Basin Candidate Division KB1 |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01266/full |
| work_keys_str_mv | AT lisamnigro phylogeographysalinityadaptationsandmetabolicpotentialofthecandidatedivisionkb1bacteriabasedonapartialsinglecellgenome AT andrewshyde phylogeographysalinityadaptationsandmetabolicpotentialofthecandidatedivisionkb1bacteriabasedonapartialsinglecellgenome AT barbarajmacgregor phylogeographysalinityadaptationsandmetabolicpotentialofthecandidatedivisionkb1bacteriabasedonapartialsinglecellgenome AT andreasteske phylogeographysalinityadaptationsandmetabolicpotentialofthecandidatedivisionkb1bacteriabasedonapartialsinglecellgenome |