Collagen-Coated Hyperelastic Bone Promotes Osteoblast Adhesion and Proliferation
Successfully reconstructing bone and restoring its dynamic function represents a significant challenge for medicine. Critical size defects (CSDs), resulting from trauma, tumor removal, or degenerative conditions, do not naturally heal and often require complex bone grafting. However, these grafts ca...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/16/21/6996 |
| _version_ | 1797631619740205056 |
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| author | Andrei Gresita Iman Raja Eugen Petcu Michael Hadjiargyrou |
| author_facet | Andrei Gresita Iman Raja Eugen Petcu Michael Hadjiargyrou |
| author_sort | Andrei Gresita |
| collection | DOAJ |
| description | Successfully reconstructing bone and restoring its dynamic function represents a significant challenge for medicine. Critical size defects (CSDs), resulting from trauma, tumor removal, or degenerative conditions, do not naturally heal and often require complex bone grafting. However, these grafts carry risks, such as tissue rejection, infections, and surgical site damage, necessitating the development of alternative treatments. Three-dimensional and four-dimensional printed synthetic biomaterials represent a viable alternative, as they carry low production costs and are highly reproducible. Hyperelastic bone (HB), a biocompatible synthetic polymer consisting of 90% hydroxyapatite and 10% poly(lactic-co-glycolic acid, PLGA), was examined for its potential to support cell adhesion, migration, and proliferation. Specifically, we seeded collagen-coated HB with MG-63 human osteosarcoma cells. Our analysis revealed robust cell adhesion and proliferation over 7 days in vitro, with cells forming uniform monolayers on the external surface of the scaffold. However, no cells were present on the core of the fibers. The cells expressed bone differentiation markers on days 3 and 5. By day 7, the scaffold began to degrade, developing microscopic fissures and fragmentation. In summary, collagen-coated HB scaffolds support cell adhesion and proliferation but exhibit reduced structural support after 7 days in culture. Nevertheless, the intricate 3D architecture holds promise for cellular migration, vascularization, and early osteogenesis. |
| first_indexed | 2024-03-11T11:25:58Z |
| format | Article |
| id | doaj.art-10c47ddbb0844e17a51707302d1c046e |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-11T11:25:58Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-10c47ddbb0844e17a51707302d1c046e2023-11-10T15:07:34ZengMDPI AGMaterials1996-19442023-11-011621699610.3390/ma16216996Collagen-Coated Hyperelastic Bone Promotes Osteoblast Adhesion and ProliferationAndrei Gresita0Iman Raja1Eugen Petcu2Michael Hadjiargyrou3Department of Biomedical Sciences, College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, NY 11568, USADepartment of Biomedical Sciences, College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, NY 11568, USADepartment of Biomedical Sciences, College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, NY 11568, USADepartment of Biological & Chemical Sciences, New York Institute of Technology, Old Westbury, NY 11568, USASuccessfully reconstructing bone and restoring its dynamic function represents a significant challenge for medicine. Critical size defects (CSDs), resulting from trauma, tumor removal, or degenerative conditions, do not naturally heal and often require complex bone grafting. However, these grafts carry risks, such as tissue rejection, infections, and surgical site damage, necessitating the development of alternative treatments. Three-dimensional and four-dimensional printed synthetic biomaterials represent a viable alternative, as they carry low production costs and are highly reproducible. Hyperelastic bone (HB), a biocompatible synthetic polymer consisting of 90% hydroxyapatite and 10% poly(lactic-co-glycolic acid, PLGA), was examined for its potential to support cell adhesion, migration, and proliferation. Specifically, we seeded collagen-coated HB with MG-63 human osteosarcoma cells. Our analysis revealed robust cell adhesion and proliferation over 7 days in vitro, with cells forming uniform monolayers on the external surface of the scaffold. However, no cells were present on the core of the fibers. The cells expressed bone differentiation markers on days 3 and 5. By day 7, the scaffold began to degrade, developing microscopic fissures and fragmentation. In summary, collagen-coated HB scaffolds support cell adhesion and proliferation but exhibit reduced structural support after 7 days in culture. Nevertheless, the intricate 3D architecture holds promise for cellular migration, vascularization, and early osteogenesis.https://www.mdpi.com/1996-1944/16/21/6996hyperelastic bone3D-printed scaffolddegradationbiocompatibilityhydroxyapatiteosteogenic differentiation |
| spellingShingle | Andrei Gresita Iman Raja Eugen Petcu Michael Hadjiargyrou Collagen-Coated Hyperelastic Bone Promotes Osteoblast Adhesion and Proliferation Materials hyperelastic bone 3D-printed scaffold degradation biocompatibility hydroxyapatite osteogenic differentiation |
| title | Collagen-Coated Hyperelastic Bone Promotes Osteoblast Adhesion and Proliferation |
| title_full | Collagen-Coated Hyperelastic Bone Promotes Osteoblast Adhesion and Proliferation |
| title_fullStr | Collagen-Coated Hyperelastic Bone Promotes Osteoblast Adhesion and Proliferation |
| title_full_unstemmed | Collagen-Coated Hyperelastic Bone Promotes Osteoblast Adhesion and Proliferation |
| title_short | Collagen-Coated Hyperelastic Bone Promotes Osteoblast Adhesion and Proliferation |
| title_sort | collagen coated hyperelastic bone promotes osteoblast adhesion and proliferation |
| topic | hyperelastic bone 3D-printed scaffold degradation biocompatibility hydroxyapatite osteogenic differentiation |
| url | https://www.mdpi.com/1996-1944/16/21/6996 |
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