UHPLC-DAD Characterization of <i>Origanum vulgare</i> L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities
The Lamiaceae family is an important source of species among medicinal plants highly valued for their biological properties and numerous uses in folk medicine. <i>Origanum</i> is one of the main genera that belong to this family. The purpose of the study was to determine the phenolic com...
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MDPI AG
2021-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/7/2100 |
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| author | Claudio Parra Patricio Muñoz Luis Bustos Felipe Parra Mario J. Simirgiotis Hugo Escobar |
| author_facet | Claudio Parra Patricio Muñoz Luis Bustos Felipe Parra Mario J. Simirgiotis Hugo Escobar |
| author_sort | Claudio Parra |
| collection | DOAJ |
| description | The Lamiaceae family is an important source of species among medicinal plants highly valued for their biological properties and numerous uses in folk medicine. <i>Origanum</i> is one of the main genera that belong to this family. The purpose of the study was to determine the phenolic composition of the <i>Origanum vulgare</i> extract and evaluate the antimicrobial, antioxidant, and inhibitory activities of this species that grows in the Andean region of the Atacama Desert. High-performance liquid chromatography was performed to determine the main phenols. Rosmarinic acid was identified as the predominant phenolic compound in this species (76.01 mg/100 g DW), followed by protocatechuic acid, which to our knowledge, no previous study reported similar concentrations in <i>O. vulgare</i>. The oregano extract exhibited a content of total phenolic (3948 mg GAE/100 g DW) and total flavonoid (593 mg QE/100 g DW) with a higher DPPH antioxidant activity (IC<sub>50</sub> = 40.58 µg/mL), compared to the same species grown under other conditions. Furthermore, it was found to inhibit α-glucosidase activity with an IC<sub>50</sub> value (7.11 mg/mL) lower than acarbose (129.32 mg/mL). <i>Pseudomonas syringae</i> and <i>Pantoea agglomerans</i> (both MIC 0.313 mg/mL and MBC 1.25 mg/mL) were the bacteria most susceptible to oregano extract with the lowest concentration necessary to inhibit bacterial growth. These results open the door for the potential use of this plant to manage chronic diseases, and they expand the knowledge of the species cultivated in arid environmental conditions. |
| first_indexed | 2024-03-10T12:33:31Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T12:33:31Z |
| publishDate | 2021-04-01 |
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| series | Molecules |
| spelling | doaj.art-10c69dae4c85409ebfd6c3d63768e8e02023-11-21T14:25:29ZengMDPI AGMolecules1420-30492021-04-01267210010.3390/molecules26072100UHPLC-DAD Characterization of <i>Origanum vulgare</i> L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition ActivitiesClaudio Parra0Patricio Muñoz1Luis Bustos2Felipe Parra3Mario J. Simirgiotis4Hugo Escobar5Laboratorio de Química Orgánica y Productos Naturales, Facultad de Ciencias Agronómicas, Universidad de Tarapacá, Av. General Velásquez 1775, Arica 1000000, ChileLaboratorio de Química Orgánica y Productos Naturales, Facultad de Ciencias Agronómicas, Universidad de Tarapacá, Av. General Velásquez 1775, Arica 1000000, ChileLaboratorio de Química Orgánica y Productos Naturales, Facultad de Ciencias Agronómicas, Universidad de Tarapacá, Av. General Velásquez 1775, Arica 1000000, ChileLaboratorio de Química Orgánica y Productos Naturales, Facultad de Ciencias Agronómicas, Universidad de Tarapacá, Av. General Velásquez 1775, Arica 1000000, ChileFacultad de Ciencias, Instituto de Farmacia, Universidad Austral de Chile, Valdivia 5110566, ChileLaboratorio de Química Orgánica y Productos Naturales, Facultad de Ciencias Agronómicas, Universidad de Tarapacá, Av. General Velásquez 1775, Arica 1000000, ChileThe Lamiaceae family is an important source of species among medicinal plants highly valued for their biological properties and numerous uses in folk medicine. <i>Origanum</i> is one of the main genera that belong to this family. The purpose of the study was to determine the phenolic composition of the <i>Origanum vulgare</i> extract and evaluate the antimicrobial, antioxidant, and inhibitory activities of this species that grows in the Andean region of the Atacama Desert. High-performance liquid chromatography was performed to determine the main phenols. Rosmarinic acid was identified as the predominant phenolic compound in this species (76.01 mg/100 g DW), followed by protocatechuic acid, which to our knowledge, no previous study reported similar concentrations in <i>O. vulgare</i>. The oregano extract exhibited a content of total phenolic (3948 mg GAE/100 g DW) and total flavonoid (593 mg QE/100 g DW) with a higher DPPH antioxidant activity (IC<sub>50</sub> = 40.58 µg/mL), compared to the same species grown under other conditions. Furthermore, it was found to inhibit α-glucosidase activity with an IC<sub>50</sub> value (7.11 mg/mL) lower than acarbose (129.32 mg/mL). <i>Pseudomonas syringae</i> and <i>Pantoea agglomerans</i> (both MIC 0.313 mg/mL and MBC 1.25 mg/mL) were the bacteria most susceptible to oregano extract with the lowest concentration necessary to inhibit bacterial growth. These results open the door for the potential use of this plant to manage chronic diseases, and they expand the knowledge of the species cultivated in arid environmental conditions.https://www.mdpi.com/1420-3049/26/7/2100α-amylaseα-glucosidasearid regionbioactive compoundsUHPLC-DADoregano |
| spellingShingle | Claudio Parra Patricio Muñoz Luis Bustos Felipe Parra Mario J. Simirgiotis Hugo Escobar UHPLC-DAD Characterization of <i>Origanum vulgare</i> L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities Molecules α-amylase α-glucosidase arid region bioactive compounds UHPLC-DAD oregano |
| title | UHPLC-DAD Characterization of <i>Origanum vulgare</i> L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities |
| title_full | UHPLC-DAD Characterization of <i>Origanum vulgare</i> L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities |
| title_fullStr | UHPLC-DAD Characterization of <i>Origanum vulgare</i> L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities |
| title_full_unstemmed | UHPLC-DAD Characterization of <i>Origanum vulgare</i> L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities |
| title_short | UHPLC-DAD Characterization of <i>Origanum vulgare</i> L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities |
| title_sort | uhplc dad characterization of i origanum vulgare i l from atacama desert andean region and antioxidant antibacterial and enzyme inhibition activities |
| topic | α-amylase α-glucosidase arid region bioactive compounds UHPLC-DAD oregano |
| url | https://www.mdpi.com/1420-3049/26/7/2100 |
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