Impact of Mutations in <i>Arabidopsis thaliana</i> Metabolic Pathways on Polerovirus Accumulation, Aphid Performance, and Feeding Behavior
During the process of virus acquisition by aphids, plants respond to both the virus and the aphids by mobilizing different metabolic pathways. It is conceivable that the plant metabolic responses to both aggressors may be conducive to virus acquisition. To address this question, we analyze the accum...
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| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/2/146 |
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| author | Florent Bogaert Aurélie Marmonier Elodie Pichon Sylvaine Boissinot Véronique Ziegler-Graff Quentin Chesnais Claire Villeroy Martin Drucker Véronique Brault |
| author_facet | Florent Bogaert Aurélie Marmonier Elodie Pichon Sylvaine Boissinot Véronique Ziegler-Graff Quentin Chesnais Claire Villeroy Martin Drucker Véronique Brault |
| author_sort | Florent Bogaert |
| collection | DOAJ |
| description | During the process of virus acquisition by aphids, plants respond to both the virus and the aphids by mobilizing different metabolic pathways. It is conceivable that the plant metabolic responses to both aggressors may be conducive to virus acquisition. To address this question, we analyze the accumulation of the phloem-limited polerovirus <i>Turnip yellows virus</i> (TuYV), which is strictly transmitted by aphids, and aphid’s life traits in six <i>Arabidopsis thaliana</i> mutants (<i>xth33</i>, <i>ss3-2</i>, <i>nata1</i>, <i>myc234</i>, <i>quad</i>, <i>atr1D,</i> and <i>pad4-1</i>). We observed that mutations affecting the carbohydrate metabolism, the synthesis of a non-protein amino acid and the glucosinolate pathway had an effect on TuYV accumulation. However, the virus titer did not correlate with the virus transmission efficiency. Some mutations in <i>A.</i> <i>thaliana</i> affect the aphid feeding behavior but often only in infected plants. The duration of the phloem sap ingestion phase, together with the time preceding the first sap ingestion, affect the virus transmission rate more than the virus titer did. Our results also show that the aphids reared on infected mutant plants had a reduced biomass regardless of the mutation and the duration of the sap ingestion phase. |
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| language | English |
| last_indexed | 2024-12-14T00:05:18Z |
| publishDate | 2020-01-01 |
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| series | Viruses |
| spelling | doaj.art-10c7a3b613e94f21b4a4296ebce3744c2022-12-21T23:26:03ZengMDPI AGViruses1999-49152020-01-0112214610.3390/v12020146v12020146Impact of Mutations in <i>Arabidopsis thaliana</i> Metabolic Pathways on Polerovirus Accumulation, Aphid Performance, and Feeding BehaviorFlorent Bogaert0Aurélie Marmonier1Elodie Pichon2Sylvaine Boissinot3Véronique Ziegler-Graff4Quentin Chesnais5Claire Villeroy6Martin Drucker7Véronique Brault8Université de Strasbourg, Institut National de Recherche en Agriculture, Alimentation et Environnement, SVQV UMR-A1131, 68000 Colmar, FranceUniversité de Strasbourg, Institut National de Recherche en Agriculture, Alimentation et Environnement, SVQV UMR-A1131, 68000 Colmar, FranceUniversité de Strasbourg, Institut National de Recherche en Agriculture, Alimentation et Environnement, SVQV UMR-A1131, 68000 Colmar, FranceUniversité de Strasbourg, Institut National de Recherche en Agriculture, Alimentation et Environnement, SVQV UMR-A1131, 68000 Colmar, FranceInstitut de biologie moléculaire des plantes, Centre National de la Recherche Scientifique, Université de Strasbourg, 67084 Strasbourg, FranceUniversité de Strasbourg, Institut National de Recherche en Agriculture, Alimentation et Environnement, SVQV UMR-A1131, 68000 Colmar, FranceUniversité de Strasbourg, Institut National de Recherche en Agriculture, Alimentation et Environnement, SVQV UMR-A1131, 68000 Colmar, FranceUniversité de Strasbourg, Institut National de Recherche en Agriculture, Alimentation et Environnement, SVQV UMR-A1131, 68000 Colmar, FranceUniversité de Strasbourg, Institut National de Recherche en Agriculture, Alimentation et Environnement, SVQV UMR-A1131, 68000 Colmar, FranceDuring the process of virus acquisition by aphids, plants respond to both the virus and the aphids by mobilizing different metabolic pathways. It is conceivable that the plant metabolic responses to both aggressors may be conducive to virus acquisition. To address this question, we analyze the accumulation of the phloem-limited polerovirus <i>Turnip yellows virus</i> (TuYV), which is strictly transmitted by aphids, and aphid’s life traits in six <i>Arabidopsis thaliana</i> mutants (<i>xth33</i>, <i>ss3-2</i>, <i>nata1</i>, <i>myc234</i>, <i>quad</i>, <i>atr1D,</i> and <i>pad4-1</i>). We observed that mutations affecting the carbohydrate metabolism, the synthesis of a non-protein amino acid and the glucosinolate pathway had an effect on TuYV accumulation. However, the virus titer did not correlate with the virus transmission efficiency. Some mutations in <i>A.</i> <i>thaliana</i> affect the aphid feeding behavior but often only in infected plants. The duration of the phloem sap ingestion phase, together with the time preceding the first sap ingestion, affect the virus transmission rate more than the virus titer did. Our results also show that the aphids reared on infected mutant plants had a reduced biomass regardless of the mutation and the duration of the sap ingestion phase.https://www.mdpi.com/1999-4915/12/2/146polerovirus<i>arabidopsis thaliana</i> mutantaphid biomasselectropenetrography (epg)transmission |
| spellingShingle | Florent Bogaert Aurélie Marmonier Elodie Pichon Sylvaine Boissinot Véronique Ziegler-Graff Quentin Chesnais Claire Villeroy Martin Drucker Véronique Brault Impact of Mutations in <i>Arabidopsis thaliana</i> Metabolic Pathways on Polerovirus Accumulation, Aphid Performance, and Feeding Behavior Viruses polerovirus <i>arabidopsis thaliana</i> mutant aphid biomass electropenetrography (epg) transmission |
| title | Impact of Mutations in <i>Arabidopsis thaliana</i> Metabolic Pathways on Polerovirus Accumulation, Aphid Performance, and Feeding Behavior |
| title_full | Impact of Mutations in <i>Arabidopsis thaliana</i> Metabolic Pathways on Polerovirus Accumulation, Aphid Performance, and Feeding Behavior |
| title_fullStr | Impact of Mutations in <i>Arabidopsis thaliana</i> Metabolic Pathways on Polerovirus Accumulation, Aphid Performance, and Feeding Behavior |
| title_full_unstemmed | Impact of Mutations in <i>Arabidopsis thaliana</i> Metabolic Pathways on Polerovirus Accumulation, Aphid Performance, and Feeding Behavior |
| title_short | Impact of Mutations in <i>Arabidopsis thaliana</i> Metabolic Pathways on Polerovirus Accumulation, Aphid Performance, and Feeding Behavior |
| title_sort | impact of mutations in i arabidopsis thaliana i metabolic pathways on polerovirus accumulation aphid performance and feeding behavior |
| topic | polerovirus <i>arabidopsis thaliana</i> mutant aphid biomass electropenetrography (epg) transmission |
| url | https://www.mdpi.com/1999-4915/12/2/146 |
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