Therapeutic effect and mechanism of Yougui Wan in rats with intervertebral disk degeneration
Abstract Objective To explore the potential mechanism of Yougui Wan on deformed lumbar intervertebral disk structure in rats. Methods Thirty male Sprague–Dawley rats were randomly divided into 3 groups, with 10 rats in each group. The animals in the blank control group were healthy rats without spec...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-024-04554-w |
| _version_ | 1827329061618188288 |
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| author | She Ma Kan Liu Jing-yan Yang Ren-jun Huang Dong Yu |
| author_facet | She Ma Kan Liu Jing-yan Yang Ren-jun Huang Dong Yu |
| author_sort | She Ma |
| collection | DOAJ |
| description | Abstract Objective To explore the potential mechanism of Yougui Wan on deformed lumbar intervertebral disk structure in rats. Methods Thirty male Sprague–Dawley rats were randomly divided into 3 groups, with 10 rats in each group. The animals in the blank control group were healthy rats without specific treatment, and those in the model group and traditional Chinese medicine (TCM) group were used to establish the intervertebral disk degeneration (IDD) model by puncturing the annulus. Four weeks after modeling, rats in the TCM group were administered Yougui Wan by gavage for 2 consecutive weeks. Serum interleukin-6 (IL-10), macrophage migration inhibitory factor (MIF) and tumor necrosis factor alpha (TNF-α) levels were measured by ELISA, and the protein expression levels of collagen II and Notch1 in intervertebral disk tissues were examined by Western blotting. Apoptosis was detected by the TUNEL method. Results Compared with those in the blank group, IL-10, MIF and TNF-α levels in the model group and TCM group were increased (P < 0.05), the protein expression levels of collagen II were decreased, and the protein expression levels of Notch1 were increased. Compared with those in the model group, the levels of IL-10 in the TCM group were increased (P < 0.05), the levels of MIF and TNF-α were decreased (P < 0.05), the protein expression levels of collagen II were increased, and the protein expression levels of Notch1 were decreased. Conclusion Yougui Wan can inhibit the inflammatory response in IDD rats, reduce the degradation of extracellular matrix, reduce apoptosis in nucleus pulposus cells, and alleviate intervertebral disk degeneration. The mechanism may be related to the regulation of the Notch signaling pathway. |
| first_indexed | 2024-03-07T15:27:26Z |
| format | Article |
| id | doaj.art-10c7c50457da44e9b88f652dade46957 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-03-07T15:27:26Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-10c7c50457da44e9b88f652dade469572024-03-05T16:36:21ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2024-01-011911610.1186/s13018-024-04554-wTherapeutic effect and mechanism of Yougui Wan in rats with intervertebral disk degenerationShe Ma0Kan Liu1Jing-yan Yang2Ren-jun Huang3Dong Yu4Beijing University of Chinese MedicineDepartment of Orthopedics, Beijing University of Chinese Medicine Third Affiliated HospitalBeijing University of Chinese MedicineBeijing University of Chinese MedicineDepartment of Orthopedics, Beijing University of Chinese Medicine Third Affiliated HospitalAbstract Objective To explore the potential mechanism of Yougui Wan on deformed lumbar intervertebral disk structure in rats. Methods Thirty male Sprague–Dawley rats were randomly divided into 3 groups, with 10 rats in each group. The animals in the blank control group were healthy rats without specific treatment, and those in the model group and traditional Chinese medicine (TCM) group were used to establish the intervertebral disk degeneration (IDD) model by puncturing the annulus. Four weeks after modeling, rats in the TCM group were administered Yougui Wan by gavage for 2 consecutive weeks. Serum interleukin-6 (IL-10), macrophage migration inhibitory factor (MIF) and tumor necrosis factor alpha (TNF-α) levels were measured by ELISA, and the protein expression levels of collagen II and Notch1 in intervertebral disk tissues were examined by Western blotting. Apoptosis was detected by the TUNEL method. Results Compared with those in the blank group, IL-10, MIF and TNF-α levels in the model group and TCM group were increased (P < 0.05), the protein expression levels of collagen II were decreased, and the protein expression levels of Notch1 were increased. Compared with those in the model group, the levels of IL-10 in the TCM group were increased (P < 0.05), the levels of MIF and TNF-α were decreased (P < 0.05), the protein expression levels of collagen II were increased, and the protein expression levels of Notch1 were decreased. Conclusion Yougui Wan can inhibit the inflammatory response in IDD rats, reduce the degradation of extracellular matrix, reduce apoptosis in nucleus pulposus cells, and alleviate intervertebral disk degeneration. The mechanism may be related to the regulation of the Notch signaling pathway.https://doi.org/10.1186/s13018-024-04554-wIntervertebral disk degenerationYougui WanNotch signaling pathwayInflammatory responseNucleus pulposus cells |
| spellingShingle | She Ma Kan Liu Jing-yan Yang Ren-jun Huang Dong Yu Therapeutic effect and mechanism of Yougui Wan in rats with intervertebral disk degeneration Journal of Orthopaedic Surgery and Research Intervertebral disk degeneration Yougui Wan Notch signaling pathway Inflammatory response Nucleus pulposus cells |
| title | Therapeutic effect and mechanism of Yougui Wan in rats with intervertebral disk degeneration |
| title_full | Therapeutic effect and mechanism of Yougui Wan in rats with intervertebral disk degeneration |
| title_fullStr | Therapeutic effect and mechanism of Yougui Wan in rats with intervertebral disk degeneration |
| title_full_unstemmed | Therapeutic effect and mechanism of Yougui Wan in rats with intervertebral disk degeneration |
| title_short | Therapeutic effect and mechanism of Yougui Wan in rats with intervertebral disk degeneration |
| title_sort | therapeutic effect and mechanism of yougui wan in rats with intervertebral disk degeneration |
| topic | Intervertebral disk degeneration Yougui Wan Notch signaling pathway Inflammatory response Nucleus pulposus cells |
| url | https://doi.org/10.1186/s13018-024-04554-w |
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