Investigation on Antioxidant Activity and Different Metabolites of Mulberry (<i>Morus</i> spp.) Leaves Depending on the Harvest Months by UPLC–Q-TOF-MS with Multivariate Tools
The changes in active components in mulberry leaves harvested in different months and their antioxidant activities were investigated. Ultra-high-performance liquid chromatography–tandem quadrupole time-of-flight mass spectrometry (UPLC–Q-TOF-MS) with multivariate statistical tools was used to invest...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/4/1947 |
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| author | Zili Guo Jiangxuan Lai Yiwen Wu Sheng Fang Xianrui Liang |
| author_facet | Zili Guo Jiangxuan Lai Yiwen Wu Sheng Fang Xianrui Liang |
| author_sort | Zili Guo |
| collection | DOAJ |
| description | The changes in active components in mulberry leaves harvested in different months and their antioxidant activities were investigated. Ultra-high-performance liquid chromatography–tandem quadrupole time-of-flight mass spectrometry (UPLC–Q-TOF-MS) with multivariate statistical tools was used to investigate the chemical constituents in the extracts of mulberry leaves. The results indicated that mulberry leaves were rich in phenolic acids, flavonoids, organic acids, and fatty acid derivatives. In addition, 25 different compounds were identified in the different batches of mulberry leaves. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was measured to evaluate the in vitro antioxidant activities of mulberry leaves. Among the four batches, batch A, harvested in December, exhibited the strongest DPPH radical-scavenging activity, while batch B, harvested in March, showed the weakest activity. This was related to the total phenolic content in the mulberry leaves of each batch. The optimal harvest time of mulberry leaves greatly influences the bioactivity and bioavailability of the plant. |
| first_indexed | 2024-03-11T08:20:43Z |
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| id | doaj.art-10c866cca8c14e37ad00f15ee218d54c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T08:20:43Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-10c866cca8c14e37ad00f15ee218d54c2023-11-16T22:25:23ZengMDPI AGMolecules1420-30492023-02-01284194710.3390/molecules28041947Investigation on Antioxidant Activity and Different Metabolites of Mulberry (<i>Morus</i> spp.) Leaves Depending on the Harvest Months by UPLC–Q-TOF-MS with Multivariate ToolsZili Guo0Jiangxuan Lai1Yiwen Wu2Sheng Fang3Xianrui Liang4Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310000, ChinaKey Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310000, ChinaKey Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310000, ChinaSchool of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, ChinaKey Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310000, ChinaThe changes in active components in mulberry leaves harvested in different months and their antioxidant activities were investigated. Ultra-high-performance liquid chromatography–tandem quadrupole time-of-flight mass spectrometry (UPLC–Q-TOF-MS) with multivariate statistical tools was used to investigate the chemical constituents in the extracts of mulberry leaves. The results indicated that mulberry leaves were rich in phenolic acids, flavonoids, organic acids, and fatty acid derivatives. In addition, 25 different compounds were identified in the different batches of mulberry leaves. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was measured to evaluate the in vitro antioxidant activities of mulberry leaves. Among the four batches, batch A, harvested in December, exhibited the strongest DPPH radical-scavenging activity, while batch B, harvested in March, showed the weakest activity. This was related to the total phenolic content in the mulberry leaves of each batch. The optimal harvest time of mulberry leaves greatly influences the bioactivity and bioavailability of the plant.https://www.mdpi.com/1420-3049/28/4/1947mulberry leafchemical constituentantioxidantUPLC–Q-TOF-MSfree radical scavenging activity |
| spellingShingle | Zili Guo Jiangxuan Lai Yiwen Wu Sheng Fang Xianrui Liang Investigation on Antioxidant Activity and Different Metabolites of Mulberry (<i>Morus</i> spp.) Leaves Depending on the Harvest Months by UPLC–Q-TOF-MS with Multivariate Tools Molecules mulberry leaf chemical constituent antioxidant UPLC–Q-TOF-MS free radical scavenging activity |
| title | Investigation on Antioxidant Activity and Different Metabolites of Mulberry (<i>Morus</i> spp.) Leaves Depending on the Harvest Months by UPLC–Q-TOF-MS with Multivariate Tools |
| title_full | Investigation on Antioxidant Activity and Different Metabolites of Mulberry (<i>Morus</i> spp.) Leaves Depending on the Harvest Months by UPLC–Q-TOF-MS with Multivariate Tools |
| title_fullStr | Investigation on Antioxidant Activity and Different Metabolites of Mulberry (<i>Morus</i> spp.) Leaves Depending on the Harvest Months by UPLC–Q-TOF-MS with Multivariate Tools |
| title_full_unstemmed | Investigation on Antioxidant Activity and Different Metabolites of Mulberry (<i>Morus</i> spp.) Leaves Depending on the Harvest Months by UPLC–Q-TOF-MS with Multivariate Tools |
| title_short | Investigation on Antioxidant Activity and Different Metabolites of Mulberry (<i>Morus</i> spp.) Leaves Depending on the Harvest Months by UPLC–Q-TOF-MS with Multivariate Tools |
| title_sort | investigation on antioxidant activity and different metabolites of mulberry i morus i spp leaves depending on the harvest months by uplc q tof ms with multivariate tools |
| topic | mulberry leaf chemical constituent antioxidant UPLC–Q-TOF-MS free radical scavenging activity |
| url | https://www.mdpi.com/1420-3049/28/4/1947 |
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