Characterization of the IncX3 Plasmid Producing blaNDM–7 From Klebsiella pneumoniae ST34
Carbapenemase-producing Klebsiella pneumoniae has been a major clinical threat worldwide because therapeutic options are limited. Although New Delhi metallo-β-lactamase (NDM) is an important carbapenemase responsible for carbapenem resistance, it is uncommon in carbapenemase-producing K. pneumoniae...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-08-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01885/full |
| _version_ | 1818128805247582208 |
|---|---|
| author | Qiong Chen Jiawei Zhou Shenghai Wu Yunxing Yang Daojun Yu Xianjun Wang Min Wu |
| author_facet | Qiong Chen Jiawei Zhou Shenghai Wu Yunxing Yang Daojun Yu Xianjun Wang Min Wu |
| author_sort | Qiong Chen |
| collection | DOAJ |
| description | Carbapenemase-producing Klebsiella pneumoniae has been a major clinical threat worldwide because therapeutic options are limited. Although New Delhi metallo-β-lactamase (NDM) is an important carbapenemase responsible for carbapenem resistance, it is uncommon in carbapenemase-producing K. pneumoniae in China. In this study, we described strain HZW25, an NDM-7-producing K. pneumoniae strain belonging to sequence type 34 (ST34). HZW25 exhibited resistance to all β-lactams tested but was susceptible to aminoglycosides and fluoroquinolones. The whole genome of HZW25 was sequenced with Pacific Biosciences RSII SMRT technology. HZW25 was composed of one chromosomal DNA and four plasmids, and the resistance genes of HZW25 were all located on the chromosome, except blaNDM–7 was located on a conjugative plasmid belonging to type IncX3 designated P4. The results of conjugation and transformation experiments showed that blaNDM–7 could be horizontally transferred successfully from the donor strain, HZW25, to the recipient strains, E. coli J53 and E. coli DH5α. The NDM variant transposable elements of the blaNDM–7-harboring plasmid P4 were the ISL3 and IS3000 families. The upstream region of blaNDM–7 contained ΔISAba125, which was inserted near the IS5 or ΔIS5 sequence. Our study is the first report of metallo-β-lactamase NDM-7 in a carbapenemase-producing K. pneumoniae strain with ST34 in China. The emergence of NDM-producing K. pneumoniae would be troublesome during treatment using ceftazidime-avibactam. Therefore, the rapid and accurate identification of carbapenemase-producing K. pneumoniae is necessary. |
| first_indexed | 2024-12-11T07:39:05Z |
| format | Article |
| id | doaj.art-10caad33c8224f04b1806e0778c2e6f3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T07:39:05Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-10caad33c8224f04b1806e0778c2e6f32022-12-22T01:15:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-08-011110.3389/fmicb.2020.01885558801Characterization of the IncX3 Plasmid Producing blaNDM–7 From Klebsiella pneumoniae ST34Qiong Chen0Jiawei Zhou1Shenghai Wu2Yunxing Yang3Daojun Yu4Xianjun Wang5Min Wu6Department of Laboratory Medicine, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Laboratory Medicine, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Laboratory Medicine, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Laboratory Medicine, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Laboratory Medicine, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Infectious Diseases, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaCarbapenemase-producing Klebsiella pneumoniae has been a major clinical threat worldwide because therapeutic options are limited. Although New Delhi metallo-β-lactamase (NDM) is an important carbapenemase responsible for carbapenem resistance, it is uncommon in carbapenemase-producing K. pneumoniae in China. In this study, we described strain HZW25, an NDM-7-producing K. pneumoniae strain belonging to sequence type 34 (ST34). HZW25 exhibited resistance to all β-lactams tested but was susceptible to aminoglycosides and fluoroquinolones. The whole genome of HZW25 was sequenced with Pacific Biosciences RSII SMRT technology. HZW25 was composed of one chromosomal DNA and four plasmids, and the resistance genes of HZW25 were all located on the chromosome, except blaNDM–7 was located on a conjugative plasmid belonging to type IncX3 designated P4. The results of conjugation and transformation experiments showed that blaNDM–7 could be horizontally transferred successfully from the donor strain, HZW25, to the recipient strains, E. coli J53 and E. coli DH5α. The NDM variant transposable elements of the blaNDM–7-harboring plasmid P4 were the ISL3 and IS3000 families. The upstream region of blaNDM–7 contained ΔISAba125, which was inserted near the IS5 or ΔIS5 sequence. Our study is the first report of metallo-β-lactamase NDM-7 in a carbapenemase-producing K. pneumoniae strain with ST34 in China. The emergence of NDM-producing K. pneumoniae would be troublesome during treatment using ceftazidime-avibactam. Therefore, the rapid and accurate identification of carbapenemase-producing K. pneumoniae is necessary.https://www.frontiersin.org/article/10.3389/fmicb.2020.01885/fullmetallo-β-lactamaseNDM-7IncX3carbapenemase-producing Klebsiella pneumoniaeceftazidime-avibactam |
| spellingShingle | Qiong Chen Jiawei Zhou Shenghai Wu Yunxing Yang Daojun Yu Xianjun Wang Min Wu Characterization of the IncX3 Plasmid Producing blaNDM–7 From Klebsiella pneumoniae ST34 Frontiers in Microbiology metallo-β-lactamase NDM-7 IncX3 carbapenemase-producing Klebsiella pneumoniae ceftazidime-avibactam |
| title | Characterization of the IncX3 Plasmid Producing blaNDM–7 From Klebsiella pneumoniae ST34 |
| title_full | Characterization of the IncX3 Plasmid Producing blaNDM–7 From Klebsiella pneumoniae ST34 |
| title_fullStr | Characterization of the IncX3 Plasmid Producing blaNDM–7 From Klebsiella pneumoniae ST34 |
| title_full_unstemmed | Characterization of the IncX3 Plasmid Producing blaNDM–7 From Klebsiella pneumoniae ST34 |
| title_short | Characterization of the IncX3 Plasmid Producing blaNDM–7 From Klebsiella pneumoniae ST34 |
| title_sort | characterization of the incx3 plasmid producing blandm 7 from klebsiella pneumoniae st34 |
| topic | metallo-β-lactamase NDM-7 IncX3 carbapenemase-producing Klebsiella pneumoniae ceftazidime-avibactam |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01885/full |
| work_keys_str_mv | AT qiongchen characterizationoftheincx3plasmidproducingblandm7fromklebsiellapneumoniaest34 AT jiaweizhou characterizationoftheincx3plasmidproducingblandm7fromklebsiellapneumoniaest34 AT shenghaiwu characterizationoftheincx3plasmidproducingblandm7fromklebsiellapneumoniaest34 AT yunxingyang characterizationoftheincx3plasmidproducingblandm7fromklebsiellapneumoniaest34 AT daojunyu characterizationoftheincx3plasmidproducingblandm7fromklebsiellapneumoniaest34 AT xianjunwang characterizationoftheincx3plasmidproducingblandm7fromklebsiellapneumoniaest34 AT minwu characterizationoftheincx3plasmidproducingblandm7fromklebsiellapneumoniaest34 |