Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs
Animal hairs are an apt surface for retention of forensic trace epithelial samples. The aim of this study was threefold: to evaluate different methods of sample collection (moistened and dry swabs) and DNA extraction (Chelex® 100 method, Qiagen EZ1® DNA Investigator Kit), as well as to examine the m...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2016-06-01
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| Series: | Acta Veterinaria |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/acve-2016-0016 |
| _version_ | 1819038422216474624 |
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| author | Špoljarić Branimira Popović Maja Crnjac Josip Žderić Savatović Zrinka Ratko Martina Lozančić Mateja Jurak Matea Špoljarić Igor Špoljarić Daniel Mršić Gordan |
| author_facet | Špoljarić Branimira Popović Maja Crnjac Josip Žderić Savatović Zrinka Ratko Martina Lozančić Mateja Jurak Matea Špoljarić Igor Špoljarić Daniel Mršić Gordan |
| author_sort | Špoljarić Branimira |
| collection | DOAJ |
| description | Animal hairs are an apt surface for retention of forensic trace epithelial samples. The aim of this study was threefold: to evaluate different methods of sample collection (moistened and dry swabs) and DNA extraction (Chelex® 100 method, Qiagen EZ1® DNA Investigator Kit), as well as to examine the morphological differences of hair fibres between two species (dog, sheep) and their ultimate impact on sample collection and processing. Our preliminary findings suggest that the use of EZ1® DNA Investigator Kit yields donor DNA profiles of higher quality. The results of different sample collection methods have shown intraspecific variations that require further investigation. The ability of retention and subsequent extraction of trace DNA appears to be similar between the two species, despite significant morphological differences between their coat hairs. |
| first_indexed | 2024-12-21T08:37:03Z |
| format | Article |
| id | doaj.art-10caddc2cc9448998bf7d6e2f080b04b |
| institution | Directory Open Access Journal |
| issn | 1820-7448 |
| language | English |
| last_indexed | 2024-12-21T08:37:03Z |
| publishDate | 2016-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Veterinaria |
| spelling | doaj.art-10caddc2cc9448998bf7d6e2f080b04b2022-12-21T19:10:03ZengSciendoActa Veterinaria1820-74482016-06-0166218720210.1515/acve-2016-0016Gleaning a Human DNA Profile from Trace Swabs Collected from Animal HairsŠpoljarić Branimira0Popović Maja1Crnjac Josip2Žderić Savatović Zrinka3Ratko Martina4Lozančić Mateja5Jurak Matea6Špoljarić Igor7Špoljarić Daniel8Mršić Gordan9Faculty of Veterinary Medicine, University of Zagreb, Zagreb, CroatiaFaculty of Veterinary Medicine, University of Zagreb, Zagreb, CroatiaUniversity Department for Forensic Sciences, University of Split, Split, CroatiaStudent of doctoral study of veterinary sciences of Faculty of Veterinary Medicine, University of Zagreb, Zagreb, CroatiaForensic Science Centre „Ivan Vučetić“, General Police Directorate, Ministry of Interior, Zagreb, CroatiaForensic Science Centre „Ivan Vučetić“, General Police Directorate, Ministry of Interior, Zagreb, CroatiaStudent of integrated undergraduate and graduate studies of Faculty of Veterinary Medicine, University of Zagreb, Zagreb, CroatiaForensic Science Centre „Ivan Vučetić“, General Police Directorate, Ministry of Interior, Zagreb, CroatiaFaculty of Veterinary Medicine, University of Zagreb, Zagreb, CroatiaForensic Science Centre „Ivan Vučetić“, General Police Directorate, Ministry of Interior, Zagreb, CroatiaAnimal hairs are an apt surface for retention of forensic trace epithelial samples. The aim of this study was threefold: to evaluate different methods of sample collection (moistened and dry swabs) and DNA extraction (Chelex® 100 method, Qiagen EZ1® DNA Investigator Kit), as well as to examine the morphological differences of hair fibres between two species (dog, sheep) and their ultimate impact on sample collection and processing. Our preliminary findings suggest that the use of EZ1® DNA Investigator Kit yields donor DNA profiles of higher quality. The results of different sample collection methods have shown intraspecific variations that require further investigation. The ability of retention and subsequent extraction of trace DNA appears to be similar between the two species, despite significant morphological differences between their coat hairs.https://doi.org/10.1515/acve-2016-0016dna profilingsheep wooldog hairchelex® 100qiagen ez1® dna investigator kit |
| spellingShingle | Špoljarić Branimira Popović Maja Crnjac Josip Žderić Savatović Zrinka Ratko Martina Lozančić Mateja Jurak Matea Špoljarić Igor Špoljarić Daniel Mršić Gordan Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs Acta Veterinaria dna profiling sheep wool dog hair chelex® 100 qiagen ez1® dna investigator kit |
| title | Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs |
| title_full | Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs |
| title_fullStr | Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs |
| title_full_unstemmed | Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs |
| title_short | Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs |
| title_sort | gleaning a human dna profile from trace swabs collected from animal hairs |
| topic | dna profiling sheep wool dog hair chelex® 100 qiagen ez1® dna investigator kit |
| url | https://doi.org/10.1515/acve-2016-0016 |
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