CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma
Xiaoyang Hou,1,* Yingkai Tao,1,* Xinxin Li,1,* Yanyu Pang,1 Chunsheng Yang,2 Guan Jiang,1 Yanqun Liu1 1Department of Dermatology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, People’s Republic of China; 2Department of Dermatology, The Affiliated Huai’an Ho...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-12-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/cd44-targeting-oxygen-self-sufficient-nanoparticles-for-enhanced-photo-peer-reviewed-article-IJN |
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| author | Hou X Tao Y Li X Pang Y Yang C Jiang G Liu Y |
| author_facet | Hou X Tao Y Li X Pang Y Yang C Jiang G Liu Y |
| author_sort | Hou X |
| collection | DOAJ |
| description | Xiaoyang Hou,1,* Yingkai Tao,1,* Xinxin Li,1,* Yanyu Pang,1 Chunsheng Yang,2 Guan Jiang,1 Yanqun Liu1 1Department of Dermatology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, People’s Republic of China; 2Department of Dermatology, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guan Jiang; Yanqun LiuDepartment of Dermatology, The Affiliated Hospital of Xuzhou Medical University, 99 West Huai Hai Road, Xuzhou, Jiangsu 221002, People’s Republic of ChinaEmail dr.guanjiang@xzhmu.edu.cn; xyfylyq@sohu.comObjective: Nanotechnology-based photodynamic therapy (PDT) is a relatively new anti-tumor strategy. However, its efficacy is limited by the hypoxic state in the tumor microenvironment. In the present study, a poly(lactic-co-glycolic acid) (PLGA) nanoparticle that encapsulated both IR820 and catalase (CAT) was developed to enhance anti-tumor therapy.Materials and Methods: HA-PLGA-CAT-IR820 nanoparticles (HCINPs) were fabricated via a double emulsion solvent evaporation method. Dynamic light scattering (DLS), transmission electron microscopy (TEM), laser scanning confocal microscopy, and an ultraviolet spectrophotometer were used to identify and characterize the nanoparticles. The stability of the nanoparticle was investigated by DLS via monitoring the sizes and polydispersity indexes (PDIs) in water, PBS, DMEM, and DMEM+10%FBS. Oxygen generation measurement was carried out via visualizing the oxygen bubbles with ultrasound imaging system and an optical microscope. Inverted fluorescence microscopy and flow cytometry were used to measure the uptake and targeting effect of the fluorescent-labeled nanoparticles. The live-dead method and tumor-bearing mouse models were applied to study the HCINP-induced enhanced PDT effect.Results: The results showed that the HCINPs could selectively target melanoma cells with high expression of CD44, and generated oxygen by catalyzing H2O2, which increased the amount of singlet oxygen, ultimately inhibiting tumor growth significantly.Conclusion: The present study presents a novel nanoplatform for melanoma treatment.Keywords: catalase, hyaluronic acid, IR820, photodynamic therapy, tumor hypoxia |
| first_indexed | 2024-12-17T13:42:11Z |
| format | Article |
| id | doaj.art-10cc05edd05f4a95a1d85d29bda382c5 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-17T13:42:11Z |
| publishDate | 2020-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-10cc05edd05f4a95a1d85d29bda382c52022-12-21T21:46:14ZengDove Medical PressInternational Journal of Nanomedicine1178-20132020-12-01Volume 15104011041660589CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant MelanomaHou XTao YLi XPang YYang CJiang GLiu YXiaoyang Hou,1,* Yingkai Tao,1,* Xinxin Li,1,* Yanyu Pang,1 Chunsheng Yang,2 Guan Jiang,1 Yanqun Liu1 1Department of Dermatology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, People’s Republic of China; 2Department of Dermatology, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guan Jiang; Yanqun LiuDepartment of Dermatology, The Affiliated Hospital of Xuzhou Medical University, 99 West Huai Hai Road, Xuzhou, Jiangsu 221002, People’s Republic of ChinaEmail dr.guanjiang@xzhmu.edu.cn; xyfylyq@sohu.comObjective: Nanotechnology-based photodynamic therapy (PDT) is a relatively new anti-tumor strategy. However, its efficacy is limited by the hypoxic state in the tumor microenvironment. In the present study, a poly(lactic-co-glycolic acid) (PLGA) nanoparticle that encapsulated both IR820 and catalase (CAT) was developed to enhance anti-tumor therapy.Materials and Methods: HA-PLGA-CAT-IR820 nanoparticles (HCINPs) were fabricated via a double emulsion solvent evaporation method. Dynamic light scattering (DLS), transmission electron microscopy (TEM), laser scanning confocal microscopy, and an ultraviolet spectrophotometer were used to identify and characterize the nanoparticles. The stability of the nanoparticle was investigated by DLS via monitoring the sizes and polydispersity indexes (PDIs) in water, PBS, DMEM, and DMEM+10%FBS. Oxygen generation measurement was carried out via visualizing the oxygen bubbles with ultrasound imaging system and an optical microscope. Inverted fluorescence microscopy and flow cytometry were used to measure the uptake and targeting effect of the fluorescent-labeled nanoparticles. The live-dead method and tumor-bearing mouse models were applied to study the HCINP-induced enhanced PDT effect.Results: The results showed that the HCINPs could selectively target melanoma cells with high expression of CD44, and generated oxygen by catalyzing H2O2, which increased the amount of singlet oxygen, ultimately inhibiting tumor growth significantly.Conclusion: The present study presents a novel nanoplatform for melanoma treatment.Keywords: catalase, hyaluronic acid, IR820, photodynamic therapy, tumor hypoxiahttps://www.dovepress.com/cd44-targeting-oxygen-self-sufficient-nanoparticles-for-enhanced-photo-peer-reviewed-article-IJNcatalasehyaluronic acidir820photodynamic therapytumor hypoxia |
| spellingShingle | Hou X Tao Y Li X Pang Y Yang C Jiang G Liu Y CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma International Journal of Nanomedicine catalase hyaluronic acid ir820 photodynamic therapy tumor hypoxia |
| title | CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma |
| title_full | CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma |
| title_fullStr | CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma |
| title_full_unstemmed | CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma |
| title_short | CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma |
| title_sort | cd44 targeting oxygen self sufficient nanoparticles for enhanced photodynamic therapy against malignant melanoma |
| topic | catalase hyaluronic acid ir820 photodynamic therapy tumor hypoxia |
| url | https://www.dovepress.com/cd44-targeting-oxygen-self-sufficient-nanoparticles-for-enhanced-photo-peer-reviewed-article-IJN |
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