Fresh Gametes Might Get Better Clinical Results than Cryopreserved Sperm or Oocytes for Nonobstructive Azoospermia Patients Underwent micro-TESE
Background: micro-testicular sperm extraction (TESE) has been the first choice for Nonobstructive azoospermia (NOA) patients for its higher sperm retrieval rate under microscope, meanwhile, sperm or oocyte cryopreservation are widely applied in in-vitro fertilization (IVF) treatment. But few researc...
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| Format: | Article |
| Language: | English |
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IMR Press
2022-06-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
| Subjects: | |
| Online Access: | https://www.imrpress.com/journal/CEOG/49/6/10.31083/j.ceog4906128 |
| _version_ | 1811334983996932096 |
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| author | Yapeng Wang Defeng Liu Xiulian Ren Shengli Lin Ming Li Hui Jiang Ping Liu |
| author_facet | Yapeng Wang Defeng Liu Xiulian Ren Shengli Lin Ming Li Hui Jiang Ping Liu |
| author_sort | Yapeng Wang |
| collection | DOAJ |
| description | Background: micro-testicular sperm extraction (TESE) has been the first choice for Nonobstructive azoospermia (NOA) patients for its higher sperm retrieval rate under microscope, meanwhile, sperm or oocyte cryopreservation are widely applied in in-vitro fertilization (IVF) treatment. But few researches have systematically explored the effect of oocyte and sperm cryopreservation in one study. Methods: we retrospectively collected and analyzed the data of fertilization and pregnancy of patients who underwent micro-TESE using fresh or vitrified-warmed gametes in our center to assess the effect of gametes cryopreservation. Results: we compared the clinical results using fresh or vitrified-warmed gametes in NOA patients after micro-TESE, respectively. We found that the the rates of fertilization and good-quality embryo using fresh gametes were 52.37 ± 24.25% and 64.00 ± 36.18%, while these using vitrified sperm or oocyte were 46.00 ± 22.70% and 68.00 ± 34.6%, 45.51 ± 25.19% and 38.57 ± 31.08%, respectively; the rates of clinical pregancy and implantation using fresh gametes were 50.0% and 32.5%, while these using vitrified sperm or oocytes were 39.5% and 32.9%, 37.5% and 24.1%, respectively. The rates of good-quality embyo and clinical pregancy of the fresh gamete were above these of the vitrified, but there was no statistic difference. The live birth rate using fresh gamete was higher than that using the vitrified (47.1% verse 32.6%, 31.3%). Conclusions: The live birth rate using fresh gamete was higher than that using vitrified gametes. Fresh gametes showed better clinical outcomes than vitrified gametes in micro-TESE-ICSI treatment for NOA patients. |
| first_indexed | 2024-04-13T17:17:14Z |
| format | Article |
| id | doaj.art-10ced49c4f5d492d85e96688fd59a31b |
| institution | Directory Open Access Journal |
| issn | 0390-6663 |
| language | English |
| last_indexed | 2024-04-13T17:17:14Z |
| publishDate | 2022-06-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj.art-10ced49c4f5d492d85e96688fd59a31b2022-12-22T02:38:05ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632022-06-0149612810.31083/j.ceog4906128S0390-6663(22)01800-0Fresh Gametes Might Get Better Clinical Results than Cryopreserved Sperm or Oocytes for Nonobstructive Azoospermia Patients Underwent micro-TESEYapeng Wang0Defeng Liu1Xiulian Ren2Shengli Lin3Ming Li4Hui Jiang5Ping Liu6Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 100191 Beijing, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 100191 Beijing, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 100191 Beijing, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 100191 Beijing, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 100191 Beijing, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 100191 Beijing, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 100191 Beijing, ChinaBackground: micro-testicular sperm extraction (TESE) has been the first choice for Nonobstructive azoospermia (NOA) patients for its higher sperm retrieval rate under microscope, meanwhile, sperm or oocyte cryopreservation are widely applied in in-vitro fertilization (IVF) treatment. But few researches have systematically explored the effect of oocyte and sperm cryopreservation in one study. Methods: we retrospectively collected and analyzed the data of fertilization and pregnancy of patients who underwent micro-TESE using fresh or vitrified-warmed gametes in our center to assess the effect of gametes cryopreservation. Results: we compared the clinical results using fresh or vitrified-warmed gametes in NOA patients after micro-TESE, respectively. We found that the the rates of fertilization and good-quality embryo using fresh gametes were 52.37 ± 24.25% and 64.00 ± 36.18%, while these using vitrified sperm or oocyte were 46.00 ± 22.70% and 68.00 ± 34.6%, 45.51 ± 25.19% and 38.57 ± 31.08%, respectively; the rates of clinical pregancy and implantation using fresh gametes were 50.0% and 32.5%, while these using vitrified sperm or oocytes were 39.5% and 32.9%, 37.5% and 24.1%, respectively. The rates of good-quality embyo and clinical pregancy of the fresh gamete were above these of the vitrified, but there was no statistic difference. The live birth rate using fresh gamete was higher than that using the vitrified (47.1% verse 32.6%, 31.3%). Conclusions: The live birth rate using fresh gamete was higher than that using vitrified gametes. Fresh gametes showed better clinical outcomes than vitrified gametes in micro-TESE-ICSI treatment for NOA patients.https://www.imrpress.com/journal/CEOG/49/6/10.31083/j.ceog4906128nonobstructive azoospermiamicro-tesesperm cryopreservationoocyte cryopreservation |
| spellingShingle | Yapeng Wang Defeng Liu Xiulian Ren Shengli Lin Ming Li Hui Jiang Ping Liu Fresh Gametes Might Get Better Clinical Results than Cryopreserved Sperm or Oocytes for Nonobstructive Azoospermia Patients Underwent micro-TESE Clinical and Experimental Obstetrics & Gynecology nonobstructive azoospermia micro-tese sperm cryopreservation oocyte cryopreservation |
| title | Fresh Gametes Might Get Better Clinical Results than Cryopreserved Sperm or Oocytes for Nonobstructive Azoospermia Patients Underwent micro-TESE |
| title_full | Fresh Gametes Might Get Better Clinical Results than Cryopreserved Sperm or Oocytes for Nonobstructive Azoospermia Patients Underwent micro-TESE |
| title_fullStr | Fresh Gametes Might Get Better Clinical Results than Cryopreserved Sperm or Oocytes for Nonobstructive Azoospermia Patients Underwent micro-TESE |
| title_full_unstemmed | Fresh Gametes Might Get Better Clinical Results than Cryopreserved Sperm or Oocytes for Nonobstructive Azoospermia Patients Underwent micro-TESE |
| title_short | Fresh Gametes Might Get Better Clinical Results than Cryopreserved Sperm or Oocytes for Nonobstructive Azoospermia Patients Underwent micro-TESE |
| title_sort | fresh gametes might get better clinical results than cryopreserved sperm or oocytes for nonobstructive azoospermia patients underwent micro tese |
| topic | nonobstructive azoospermia micro-tese sperm cryopreservation oocyte cryopreservation |
| url | https://www.imrpress.com/journal/CEOG/49/6/10.31083/j.ceog4906128 |
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