Serum Extracellular Vesicle-Derived hsa-miR-2277-3p and hsa-miR-6813-3p Are Potential Biomarkers for Major Depression: A Preliminary Study
The aim of the present study was to examine the association between miRNA levels in extracellular vesicles (EVs) from serum and the severity of Major Depression (MD). Patient sera from 16 MD cases were collected at our university hospital. The miRNAs contained in EVs were extracted using a nanofiltr...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13902 |
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| author | Issei Seki Hiroto Izumi Naomichi Okamoto Atsuko Ikenouchi Yasuo Morimoto Seichi Horie Reiji Yoshimura |
| author_facet | Issei Seki Hiroto Izumi Naomichi Okamoto Atsuko Ikenouchi Yasuo Morimoto Seichi Horie Reiji Yoshimura |
| author_sort | Issei Seki |
| collection | DOAJ |
| description | The aim of the present study was to examine the association between miRNA levels in extracellular vesicles (EVs) from serum and the severity of Major Depression (MD). Patient sera from 16 MD cases were collected at our university hospital. The miRNAs contained in EVs were extracted using a nanofiltration method, and their expression levels were analyzed using miRNA microarrays. Intergroup comparisons were performed to validate the diagnostic performance of miRNAs in EVs. Furthermore, candidate miRNAs in EVs were added to neural progenitor cells, astrocytes, and microglial cells in vitro, and the predicted target genes of the candidate miRNAs were extracted. The predicted target genes underwent enrichment analysis. The expression levels of hsa-miR-6813-3p and hsa-miR-2277-3p were significantly downregulated with increasing depression severity of MD. The pathway enrichment analysis suggests that hsa-miR-6813-3p may be involved in glucocorticoid receptor and gamma-aminobutyric acid receptor signaling. Additionally, hsa-miR-2277-3p was found to be involved in the dopaminergic neural pathway. The analysis of serum miRNAs in EVs suggests that hsa-miR-6813-3p and hsa-miR-2277-3p could serve as novel biomarkers for MD, reflecting its severity. Moreover, these miRNAs in EVs could help understand MD pathophysiology. |
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| id | doaj.art-10d19b3f7e7b48ef9b36eaf9f8e7b484 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:39:56Z |
| publishDate | 2023-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-10d19b3f7e7b48ef9b36eaf9f8e7b4842023-11-19T11:04:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181390210.3390/ijms241813902Serum Extracellular Vesicle-Derived hsa-miR-2277-3p and hsa-miR-6813-3p Are Potential Biomarkers for Major Depression: A Preliminary StudyIssei Seki0Hiroto Izumi1Naomichi Okamoto2Atsuko Ikenouchi3Yasuo Morimoto4Seichi Horie5Reiji Yoshimura6Department of Psychiatry, University of Occupational and Environmental Health, Kitakyusyu 807-8555, JapanCenter for Stress-related Disease Control and Prevention, University of Occupational and Environmental Health, Kitakyusyu 807-8555, JapanDepartment of Psychiatry, University of Occupational and Environmental Health, Kitakyusyu 807-8555, JapanDepartment of Psychiatry, University of Occupational and Environmental Health, Kitakyusyu 807-8555, JapanCenter for Stress-related Disease Control and Prevention, University of Occupational and Environmental Health, Kitakyusyu 807-8555, JapanCenter for Stress-related Disease Control and Prevention, University of Occupational and Environmental Health, Kitakyusyu 807-8555, JapanDepartment of Psychiatry, University of Occupational and Environmental Health, Kitakyusyu 807-8555, JapanThe aim of the present study was to examine the association between miRNA levels in extracellular vesicles (EVs) from serum and the severity of Major Depression (MD). Patient sera from 16 MD cases were collected at our university hospital. The miRNAs contained in EVs were extracted using a nanofiltration method, and their expression levels were analyzed using miRNA microarrays. Intergroup comparisons were performed to validate the diagnostic performance of miRNAs in EVs. Furthermore, candidate miRNAs in EVs were added to neural progenitor cells, astrocytes, and microglial cells in vitro, and the predicted target genes of the candidate miRNAs were extracted. The predicted target genes underwent enrichment analysis. The expression levels of hsa-miR-6813-3p and hsa-miR-2277-3p were significantly downregulated with increasing depression severity of MD. The pathway enrichment analysis suggests that hsa-miR-6813-3p may be involved in glucocorticoid receptor and gamma-aminobutyric acid receptor signaling. Additionally, hsa-miR-2277-3p was found to be involved in the dopaminergic neural pathway. The analysis of serum miRNAs in EVs suggests that hsa-miR-6813-3p and hsa-miR-2277-3p could serve as novel biomarkers for MD, reflecting its severity. Moreover, these miRNAs in EVs could help understand MD pathophysiology.https://www.mdpi.com/1422-0067/24/18/13902major depressionextracellular vesicleshsa-miR-6813-3phsa-miR-2277-3pserum |
| spellingShingle | Issei Seki Hiroto Izumi Naomichi Okamoto Atsuko Ikenouchi Yasuo Morimoto Seichi Horie Reiji Yoshimura Serum Extracellular Vesicle-Derived hsa-miR-2277-3p and hsa-miR-6813-3p Are Potential Biomarkers for Major Depression: A Preliminary Study International Journal of Molecular Sciences major depression extracellular vesicles hsa-miR-6813-3p hsa-miR-2277-3p serum |
| title | Serum Extracellular Vesicle-Derived hsa-miR-2277-3p and hsa-miR-6813-3p Are Potential Biomarkers for Major Depression: A Preliminary Study |
| title_full | Serum Extracellular Vesicle-Derived hsa-miR-2277-3p and hsa-miR-6813-3p Are Potential Biomarkers for Major Depression: A Preliminary Study |
| title_fullStr | Serum Extracellular Vesicle-Derived hsa-miR-2277-3p and hsa-miR-6813-3p Are Potential Biomarkers for Major Depression: A Preliminary Study |
| title_full_unstemmed | Serum Extracellular Vesicle-Derived hsa-miR-2277-3p and hsa-miR-6813-3p Are Potential Biomarkers for Major Depression: A Preliminary Study |
| title_short | Serum Extracellular Vesicle-Derived hsa-miR-2277-3p and hsa-miR-6813-3p Are Potential Biomarkers for Major Depression: A Preliminary Study |
| title_sort | serum extracellular vesicle derived hsa mir 2277 3p and hsa mir 6813 3p are potential biomarkers for major depression a preliminary study |
| topic | major depression extracellular vesicles hsa-miR-6813-3p hsa-miR-2277-3p serum |
| url | https://www.mdpi.com/1422-0067/24/18/13902 |
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