Differential Post-Translational Modifications of Proteins in Bladder Ischemia
Clinical and basic research suggests that bladder ischemia may be an independent variable in the development of lower urinary tract symptoms (LUTS). We have reported that ischemic changes in the bladder involve differential expression and post-translational modifications (PTMs) of the protein’s func...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/12/1/81 |
| _version_ | 1797340058466910208 |
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| author | Han-Pil Choi Jing-Hua Yang Kazem M. Azadzoi |
| author_facet | Han-Pil Choi Jing-Hua Yang Kazem M. Azadzoi |
| author_sort | Han-Pil Choi |
| collection | DOAJ |
| description | Clinical and basic research suggests that bladder ischemia may be an independent variable in the development of lower urinary tract symptoms (LUTS). We have reported that ischemic changes in the bladder involve differential expression and post-translational modifications (PTMs) of the protein’s functional domains. In the present study, we performed in-depth analysis of a previously reported proteomic dataset to further characterize proteins PTMs in bladder ischemia. Our proteomic analysis of proteins in bladder ischemia detected differential formation of non-coded amino acids (ncAAs) that might have resulted from PTMs. In-depth analysis revealed that three groups of proteins in the bladder proteome, including contractile proteins and their associated proteins, stress response proteins, and cell signaling-related proteins, are conspicuously impacted by ischemia. Differential PTMs of proteins by ischemia seemed to affect important signaling pathways in the bladder and provoke critical changes in the post-translational structural integrity of the stress response, contractile, and cell signaling-related proteins. Our data suggest that differential PTMs of proteins may play a role in the development of cellular stress, sensitization of smooth muscle cells to contractile stimuli, and deferential cell signaling in bladder ischemia. These observations may provide the foundation for future research to validate and define clinical translation of the modified biomarkers for precise diagnosis of bladder dysfunction and the development of new therapeutic targets against LUTS. |
| first_indexed | 2024-03-08T09:57:27Z |
| format | Article |
| id | doaj.art-10d24a0f21d24dd6a7010d0d3a24c1b9 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-08T09:57:27Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-10d24a0f21d24dd6a7010d0d3a24c1b92024-01-29T13:46:58ZengMDPI AGBiomedicines2227-90592023-12-011218110.3390/biomedicines12010081Differential Post-Translational Modifications of Proteins in Bladder IschemiaHan-Pil Choi0Jing-Hua Yang1Kazem M. Azadzoi2Proteomics Laboratory, VA Boston Healthcare System, Boston, MA 02130, USAProteomics Laboratory, Department of Surgery, VA Boston Healthcare System, Boston University School of Medicine, Boston, MA 02130, USADepartments of Urology and Pathology, VA Boston Healthcare System, Boston University School of Medicine, Boston, MA 02130, USAClinical and basic research suggests that bladder ischemia may be an independent variable in the development of lower urinary tract symptoms (LUTS). We have reported that ischemic changes in the bladder involve differential expression and post-translational modifications (PTMs) of the protein’s functional domains. In the present study, we performed in-depth analysis of a previously reported proteomic dataset to further characterize proteins PTMs in bladder ischemia. Our proteomic analysis of proteins in bladder ischemia detected differential formation of non-coded amino acids (ncAAs) that might have resulted from PTMs. In-depth analysis revealed that three groups of proteins in the bladder proteome, including contractile proteins and their associated proteins, stress response proteins, and cell signaling-related proteins, are conspicuously impacted by ischemia. Differential PTMs of proteins by ischemia seemed to affect important signaling pathways in the bladder and provoke critical changes in the post-translational structural integrity of the stress response, contractile, and cell signaling-related proteins. Our data suggest that differential PTMs of proteins may play a role in the development of cellular stress, sensitization of smooth muscle cells to contractile stimuli, and deferential cell signaling in bladder ischemia. These observations may provide the foundation for future research to validate and define clinical translation of the modified biomarkers for precise diagnosis of bladder dysfunction and the development of new therapeutic targets against LUTS.https://www.mdpi.com/2227-9059/12/1/81bladderischemiaprotein profilingpost-translational modificationsnon-coded amino acids |
| spellingShingle | Han-Pil Choi Jing-Hua Yang Kazem M. Azadzoi Differential Post-Translational Modifications of Proteins in Bladder Ischemia Biomedicines bladder ischemia protein profiling post-translational modifications non-coded amino acids |
| title | Differential Post-Translational Modifications of Proteins in Bladder Ischemia |
| title_full | Differential Post-Translational Modifications of Proteins in Bladder Ischemia |
| title_fullStr | Differential Post-Translational Modifications of Proteins in Bladder Ischemia |
| title_full_unstemmed | Differential Post-Translational Modifications of Proteins in Bladder Ischemia |
| title_short | Differential Post-Translational Modifications of Proteins in Bladder Ischemia |
| title_sort | differential post translational modifications of proteins in bladder ischemia |
| topic | bladder ischemia protein profiling post-translational modifications non-coded amino acids |
| url | https://www.mdpi.com/2227-9059/12/1/81 |
| work_keys_str_mv | AT hanpilchoi differentialposttranslationalmodificationsofproteinsinbladderischemia AT jinghuayang differentialposttranslationalmodificationsofproteinsinbladderischemia AT kazemmazadzoi differentialposttranslationalmodificationsofproteinsinbladderischemia |