Bone marrow-derived cells can acquire renal stem cells properties and ameliorate ischemia-reperfusion induced acute renal injury
<p>Abstract</p> <p>Background</p> <p>Bone marrow (BM) stem cells have been reported to contribute to tissue repair after kidney injury model. However, there is no direct evidence so far that BM cells can trans-differentiate into renal stem cells.</p> <p>Meth...
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| Format: | Article |
| Language: | English |
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BMC
2012-09-01
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| Series: | BMC Nephrology |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2369/13/105 |
| _version_ | 1818691154018828288 |
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| author | Jia Xiaohua Xie Xiaoqiang Feng Guowei Lű He Zhao Qinjun Che Yongzhe Zheng Yizhou Han Zhongchao Xu Yong Li Zongjin Kong Deling |
| author_facet | Jia Xiaohua Xie Xiaoqiang Feng Guowei Lű He Zhao Qinjun Che Yongzhe Zheng Yizhou Han Zhongchao Xu Yong Li Zongjin Kong Deling |
| author_sort | Jia Xiaohua |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Bone marrow (BM) stem cells have been reported to contribute to tissue repair after kidney injury model. However, there is no direct evidence so far that BM cells can trans-differentiate into renal stem cells.</p> <p>Methods</p> <p>To investigate whether BM stem cells contribute to repopulate the renal stem cell pool, we transplanted BM cells from transgenic mice, expressing enhanced green fluorescent protein (EGFP) into wild-type irradiated recipients. Following hematological reconstitution and ischemia-reperfusion (I/R), Sca-1 and c-Kit positive renal stem cells in kidney were evaluated by immunostaining and flow cytometry analysis. Moreover, granulocyte colony stimulating factor (G-CSF) was administrated to further explore if G-CSF can mobilize BM cells and enhance trans-differentiation efficiency of BM cells into renal stem cells.</p> <p>Results</p> <p>BM-derived cells can contribute to the Sca-1<sup>+</sup> or c-Kit<sup>+</sup> renal progenitor cells population, although most renal stem cells came from indigenous cells. Furthermore, G-CSF administration nearly doubled the frequency of Sca-1+ BM-derived renal stem cells and increased capillary density of I/R injured kidneys.</p> <p>Conclusions</p> <p>These findings indicate that BM derived stem cells can give rise to cells that share properties of renal resident stem cell. Moreover, G-CSF mobilization can enhance this effect.</p> |
| first_indexed | 2024-12-17T12:37:23Z |
| format | Article |
| id | doaj.art-10d310cf188048c0b10cf9fc711f1520 |
| institution | Directory Open Access Journal |
| issn | 1471-2369 |
| language | English |
| last_indexed | 2024-12-17T12:37:23Z |
| publishDate | 2012-09-01 |
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| series | BMC Nephrology |
| spelling | doaj.art-10d310cf188048c0b10cf9fc711f15202022-12-21T21:48:13ZengBMCBMC Nephrology1471-23692012-09-0113110510.1186/1471-2369-13-105Bone marrow-derived cells can acquire renal stem cells properties and ameliorate ischemia-reperfusion induced acute renal injuryJia XiaohuaXie XiaoqiangFeng GuoweiLű HeZhao QinjunChe YongzheZheng YizhouHan ZhongchaoXu YongLi ZongjinKong Deling<p>Abstract</p> <p>Background</p> <p>Bone marrow (BM) stem cells have been reported to contribute to tissue repair after kidney injury model. However, there is no direct evidence so far that BM cells can trans-differentiate into renal stem cells.</p> <p>Methods</p> <p>To investigate whether BM stem cells contribute to repopulate the renal stem cell pool, we transplanted BM cells from transgenic mice, expressing enhanced green fluorescent protein (EGFP) into wild-type irradiated recipients. Following hematological reconstitution and ischemia-reperfusion (I/R), Sca-1 and c-Kit positive renal stem cells in kidney were evaluated by immunostaining and flow cytometry analysis. Moreover, granulocyte colony stimulating factor (G-CSF) was administrated to further explore if G-CSF can mobilize BM cells and enhance trans-differentiation efficiency of BM cells into renal stem cells.</p> <p>Results</p> <p>BM-derived cells can contribute to the Sca-1<sup>+</sup> or c-Kit<sup>+</sup> renal progenitor cells population, although most renal stem cells came from indigenous cells. Furthermore, G-CSF administration nearly doubled the frequency of Sca-1+ BM-derived renal stem cells and increased capillary density of I/R injured kidneys.</p> <p>Conclusions</p> <p>These findings indicate that BM derived stem cells can give rise to cells that share properties of renal resident stem cell. Moreover, G-CSF mobilization can enhance this effect.</p>http://www.biomedcentral.com/1471-2369/13/105Bone marrow transplantationTrans-differentiationRenal stem cellAcute kidney injuryG-CSFMobilization |
| spellingShingle | Jia Xiaohua Xie Xiaoqiang Feng Guowei Lű He Zhao Qinjun Che Yongzhe Zheng Yizhou Han Zhongchao Xu Yong Li Zongjin Kong Deling Bone marrow-derived cells can acquire renal stem cells properties and ameliorate ischemia-reperfusion induced acute renal injury BMC Nephrology Bone marrow transplantation Trans-differentiation Renal stem cell Acute kidney injury G-CSF Mobilization |
| title | Bone marrow-derived cells can acquire renal stem cells properties and ameliorate ischemia-reperfusion induced acute renal injury |
| title_full | Bone marrow-derived cells can acquire renal stem cells properties and ameliorate ischemia-reperfusion induced acute renal injury |
| title_fullStr | Bone marrow-derived cells can acquire renal stem cells properties and ameliorate ischemia-reperfusion induced acute renal injury |
| title_full_unstemmed | Bone marrow-derived cells can acquire renal stem cells properties and ameliorate ischemia-reperfusion induced acute renal injury |
| title_short | Bone marrow-derived cells can acquire renal stem cells properties and ameliorate ischemia-reperfusion induced acute renal injury |
| title_sort | bone marrow derived cells can acquire renal stem cells properties and ameliorate ischemia reperfusion induced acute renal injury |
| topic | Bone marrow transplantation Trans-differentiation Renal stem cell Acute kidney injury G-CSF Mobilization |
| url | http://www.biomedcentral.com/1471-2369/13/105 |
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