FIP1 Plays an Important Role in Nitrate Signaling and Regulates CIPK8 and CIPK23 Expression in Arabidopsis
Unraveling the molecular mechanisms of nitrate regulation and deciphering the underlying genetic network is vital for elucidating nitrate uptake and utilization in plants. Such knowledge could lead to the improvement of nitrogen-use efficiency in agriculture. Here, we report that the FIP1 gene (fact...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00593/full |
| _version_ | 1819181439702269952 |
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| author | Chao Wang Chao Wang Wenjing Zhang Zehui Li Zhen Li Yingjun Bi Nigel M. Crawford Yong Wang |
| author_facet | Chao Wang Chao Wang Wenjing Zhang Zehui Li Zhen Li Yingjun Bi Nigel M. Crawford Yong Wang |
| author_sort | Chao Wang |
| collection | DOAJ |
| description | Unraveling the molecular mechanisms of nitrate regulation and deciphering the underlying genetic network is vital for elucidating nitrate uptake and utilization in plants. Such knowledge could lead to the improvement of nitrogen-use efficiency in agriculture. Here, we report that the FIP1 gene (factor interacting with poly(A) polymerase 1) plays an important role in nitrate signaling in Arabidopsis thaliana. FIP1 encodes a putative core component of the polyadenylation factor complex. We found that FIP1 interacts with the cleavage and polyadenylation specificity factor 30-L (CPSF30-L), which is also an essential player in nitrate signaling. The induction of nitrate-responsive genes following nitrate treatment was inhibited in the fip1 mutant. The nitrate content was also reduced in fip1 seedlings due to their decreased nitrate uptake activity. Furthermore, the nitrate content was higher in the roots but lower in the roots of fip1, which may result from the downregulation of NRT1.8 and the upregulation of the nitrate assimilation genes. In addition, qPCR analyses revealed that FIP1 negatively regulated the expression of CIPK8 and CIPK23, two protein kinases involved in nitrate signaling. In the fip1 mutant, the increased expression of CIPK23 may affect nitrate uptake, resulting in its lower nitrate content. Genetic and molecular evidence suggests that FIP1 and CPSF30-L function in the same nitrate-signaling pathway, with FIP1 mediating signaling through its interaction with CPSF30-L and its regulation of CIPK8 and CIPK23. Analysis of the 3′-UTR of NRT1.1 showed that the pattern of polyadenylation sites was altered in the fip1 mutant. These findings add a novel component to the nitrate regulation network and enhance our understanding of the underlying mechanisms for nitrate signaling. |
| first_indexed | 2024-12-22T22:30:15Z |
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| language | English |
| last_indexed | 2024-12-22T22:30:15Z |
| publishDate | 2018-05-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-10d406c8d00d41159a3ac203350442792022-12-21T18:10:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-05-01910.3389/fpls.2018.00593366719FIP1 Plays an Important Role in Nitrate Signaling and Regulates CIPK8 and CIPK23 Expression in ArabidopsisChao Wang0Chao Wang1Wenjing Zhang2Zehui Li3Zhen Li4Yingjun Bi5Nigel M. Crawford6Yong Wang7National Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, ChinaSection of General Biology, Department of Life Science and Engineering, Jining University, Jining, ChinaNational Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, ChinaNational Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, ChinaNational Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, ChinaNational Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, ChinaSection of Cell and Developmental Biology, Division of Biological Science, University of California at San Diego, La Jolla, CA, United StatesNational Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, ChinaUnraveling the molecular mechanisms of nitrate regulation and deciphering the underlying genetic network is vital for elucidating nitrate uptake and utilization in plants. Such knowledge could lead to the improvement of nitrogen-use efficiency in agriculture. Here, we report that the FIP1 gene (factor interacting with poly(A) polymerase 1) plays an important role in nitrate signaling in Arabidopsis thaliana. FIP1 encodes a putative core component of the polyadenylation factor complex. We found that FIP1 interacts with the cleavage and polyadenylation specificity factor 30-L (CPSF30-L), which is also an essential player in nitrate signaling. The induction of nitrate-responsive genes following nitrate treatment was inhibited in the fip1 mutant. The nitrate content was also reduced in fip1 seedlings due to their decreased nitrate uptake activity. Furthermore, the nitrate content was higher in the roots but lower in the roots of fip1, which may result from the downregulation of NRT1.8 and the upregulation of the nitrate assimilation genes. In addition, qPCR analyses revealed that FIP1 negatively regulated the expression of CIPK8 and CIPK23, two protein kinases involved in nitrate signaling. In the fip1 mutant, the increased expression of CIPK23 may affect nitrate uptake, resulting in its lower nitrate content. Genetic and molecular evidence suggests that FIP1 and CPSF30-L function in the same nitrate-signaling pathway, with FIP1 mediating signaling through its interaction with CPSF30-L and its regulation of CIPK8 and CIPK23. Analysis of the 3′-UTR of NRT1.1 showed that the pattern of polyadenylation sites was altered in the fip1 mutant. These findings add a novel component to the nitrate regulation network and enhance our understanding of the underlying mechanisms for nitrate signaling.http://journal.frontiersin.org/article/10.3389/fpls.2018.00593/fullArabidopsisFIP1CPSF30-Lnitrate signalingnitrate uptake and assimilationCIPK8 |
| spellingShingle | Chao Wang Chao Wang Wenjing Zhang Zehui Li Zhen Li Yingjun Bi Nigel M. Crawford Yong Wang FIP1 Plays an Important Role in Nitrate Signaling and Regulates CIPK8 and CIPK23 Expression in Arabidopsis Frontiers in Plant Science Arabidopsis FIP1 CPSF30-L nitrate signaling nitrate uptake and assimilation CIPK8 |
| title | FIP1 Plays an Important Role in Nitrate Signaling and Regulates CIPK8 and CIPK23 Expression in Arabidopsis |
| title_full | FIP1 Plays an Important Role in Nitrate Signaling and Regulates CIPK8 and CIPK23 Expression in Arabidopsis |
| title_fullStr | FIP1 Plays an Important Role in Nitrate Signaling and Regulates CIPK8 and CIPK23 Expression in Arabidopsis |
| title_full_unstemmed | FIP1 Plays an Important Role in Nitrate Signaling and Regulates CIPK8 and CIPK23 Expression in Arabidopsis |
| title_short | FIP1 Plays an Important Role in Nitrate Signaling and Regulates CIPK8 and CIPK23 Expression in Arabidopsis |
| title_sort | fip1 plays an important role in nitrate signaling and regulates cipk8 and cipk23 expression in arabidopsis |
| topic | Arabidopsis FIP1 CPSF30-L nitrate signaling nitrate uptake and assimilation CIPK8 |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00593/full |
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