Screening for fecal carriage of MCR-producing Enterobacteriaceae in healthy humans and primary care patients
Abstract Background The extent of the occurrence of the plasmid-encoded colistin resistance genes mcr-1 and mcr-2 among humans is currently sparsely studied in Western Europe. Objectives To determine the occurrence of MCR-producing Enterobacteriaceae in fecal samples of healthy humans with high occu...
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| Format: | Article |
| Language: | English |
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BMC
2017-03-01
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| Series: | Antimicrobial Resistance and Infection Control |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13756-017-0186-z |
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| author | Katrin Zurfluh Roger Stephan Andreas Widmer Laurent Poirel Patrice Nordmann Hans-Jakob Nüesch Herbert Hächler Magdalena Nüesch-Inderbinen |
| author_facet | Katrin Zurfluh Roger Stephan Andreas Widmer Laurent Poirel Patrice Nordmann Hans-Jakob Nüesch Herbert Hächler Magdalena Nüesch-Inderbinen |
| author_sort | Katrin Zurfluh |
| collection | DOAJ |
| description | Abstract Background The extent of the occurrence of the plasmid-encoded colistin resistance genes mcr-1 and mcr-2 among humans is currently sparsely studied in Western Europe. Objectives To determine the occurrence of MCR-producing Enterobacteriaceae in fecal samples of healthy humans with high occupational exposure to food and primary care patients in Switzerland. Methods Stool samples from 1091 healthy individuals and fecal swabs from 53 primary care patients were screened for polymyxin-resistant Enterobacteriaceae using LB agar containing 4 mg/L colistin. Minimal inhibitory concentrations (MICs) of colistin were determined for non-intrinsic colistin-resistant isolates. Isolates were screened by PCR for the presence of mcr-1 and mcr-2 genes. Results The fecal carriage rate of colistin resistant (MIC value >2 mg/l) Enterobacteriaceae was 1.5% for healthy people and 3.8% for primary care patients. Isolates included Hafnia alvei (n = 9), Escherichia coli (n = 3), Enterobacter cloacae (n = 4), Klebsiella pneumoniae (n = 1) and Raoultella ornithinolytica (n = 1). None of the isolates harbored the mcr-1 or mcr-2 genes. Conclusions There is no evidence for the presence of MCR-producers in the fecal flora of healthy people or primary care patients. Therefore, the risk of transfer of mcr genes from animals, food or the environment to humans is likely to be low in Switzerland. |
| first_indexed | 2024-04-14T02:08:49Z |
| format | Article |
| id | doaj.art-10d748b9d0fa4b07b50fa2f6a547bb45 |
| institution | Directory Open Access Journal |
| issn | 2047-2994 |
| language | English |
| last_indexed | 2024-04-14T02:08:49Z |
| publishDate | 2017-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Antimicrobial Resistance and Infection Control |
| spelling | doaj.art-10d748b9d0fa4b07b50fa2f6a547bb452022-12-22T02:18:33ZengBMCAntimicrobial Resistance and Infection Control2047-29942017-03-01611410.1186/s13756-017-0186-zScreening for fecal carriage of MCR-producing Enterobacteriaceae in healthy humans and primary care patientsKatrin Zurfluh0Roger Stephan1Andreas Widmer2Laurent Poirel3Patrice Nordmann4Hans-Jakob Nüesch5Herbert Hächler6Magdalena Nüesch-Inderbinen7Institute for Food Safety and Hygiene, Vetsuisse Faculty University of ZurichInstitute for Food Safety and Hygiene, Vetsuisse Faculty University of ZurichDivision of Infectious Diseases and Hospital Epidemiology, University of BaselEmerging Antibiotic Resistance, Medical and Molecular Microbiology Unit, Department of Medicine, University of FribourgEmerging Antibiotic Resistance, Medical and Molecular Microbiology Unit, Department of Medicine, University of FribourgPractice for General MedicineInstitute for Food Safety and Hygiene, Vetsuisse Faculty University of ZurichInstitute for Food Safety and Hygiene, Vetsuisse Faculty University of ZurichAbstract Background The extent of the occurrence of the plasmid-encoded colistin resistance genes mcr-1 and mcr-2 among humans is currently sparsely studied in Western Europe. Objectives To determine the occurrence of MCR-producing Enterobacteriaceae in fecal samples of healthy humans with high occupational exposure to food and primary care patients in Switzerland. Methods Stool samples from 1091 healthy individuals and fecal swabs from 53 primary care patients were screened for polymyxin-resistant Enterobacteriaceae using LB agar containing 4 mg/L colistin. Minimal inhibitory concentrations (MICs) of colistin were determined for non-intrinsic colistin-resistant isolates. Isolates were screened by PCR for the presence of mcr-1 and mcr-2 genes. Results The fecal carriage rate of colistin resistant (MIC value >2 mg/l) Enterobacteriaceae was 1.5% for healthy people and 3.8% for primary care patients. Isolates included Hafnia alvei (n = 9), Escherichia coli (n = 3), Enterobacter cloacae (n = 4), Klebsiella pneumoniae (n = 1) and Raoultella ornithinolytica (n = 1). None of the isolates harbored the mcr-1 or mcr-2 genes. Conclusions There is no evidence for the presence of MCR-producers in the fecal flora of healthy people or primary care patients. Therefore, the risk of transfer of mcr genes from animals, food or the environment to humans is likely to be low in Switzerland.http://link.springer.com/article/10.1186/s13756-017-0186-zColistinMCRFecal carriagePopulation |
| spellingShingle | Katrin Zurfluh Roger Stephan Andreas Widmer Laurent Poirel Patrice Nordmann Hans-Jakob Nüesch Herbert Hächler Magdalena Nüesch-Inderbinen Screening for fecal carriage of MCR-producing Enterobacteriaceae in healthy humans and primary care patients Antimicrobial Resistance and Infection Control Colistin MCR Fecal carriage Population |
| title | Screening for fecal carriage of MCR-producing Enterobacteriaceae in healthy humans and primary care patients |
| title_full | Screening for fecal carriage of MCR-producing Enterobacteriaceae in healthy humans and primary care patients |
| title_fullStr | Screening for fecal carriage of MCR-producing Enterobacteriaceae in healthy humans and primary care patients |
| title_full_unstemmed | Screening for fecal carriage of MCR-producing Enterobacteriaceae in healthy humans and primary care patients |
| title_short | Screening for fecal carriage of MCR-producing Enterobacteriaceae in healthy humans and primary care patients |
| title_sort | screening for fecal carriage of mcr producing enterobacteriaceae in healthy humans and primary care patients |
| topic | Colistin MCR Fecal carriage Population |
| url | http://link.springer.com/article/10.1186/s13756-017-0186-z |
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