Exoproteome and secretome derived broad spectrum novel drug and vaccine candidates in Vibrio cholerae targeted by Piper betel derived compounds.
Vibrio cholerae is the causal organism of the cholera epidemic, which is mostly prevalent in developing and underdeveloped countries. However, incidences of cholera in developed countries are also alarming. Because of the emergence of new drug-resistant strains, even though several generic drugs and...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3559646?pdf=render |
| _version_ | 1818142286398095360 |
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| author | Debmalya Barh Neha Barve Krishnakant Gupta Sudha Chandra Neha Jain Sandeep Tiwari Nidia Leon-Sicairos Adrian Canizalez-Roman Anderson Rodrigues dos Santos Syed Shah Hassan Síntia Almeida Rommel Thiago Jucá Ramos Vinicius Augusto Carvalho de Abreu Adriana Ribeiro Carneiro Siomar de Castro Soares Thiago Luiz de Paula Castro Anderson Miyoshi Artur Silva Anil Kumar Amarendra Narayan Misra Kenneth Blum Eric R Braverman Vasco Azevedo |
| author_facet | Debmalya Barh Neha Barve Krishnakant Gupta Sudha Chandra Neha Jain Sandeep Tiwari Nidia Leon-Sicairos Adrian Canizalez-Roman Anderson Rodrigues dos Santos Syed Shah Hassan Síntia Almeida Rommel Thiago Jucá Ramos Vinicius Augusto Carvalho de Abreu Adriana Ribeiro Carneiro Siomar de Castro Soares Thiago Luiz de Paula Castro Anderson Miyoshi Artur Silva Anil Kumar Amarendra Narayan Misra Kenneth Blum Eric R Braverman Vasco Azevedo |
| author_sort | Debmalya Barh |
| collection | DOAJ |
| description | Vibrio cholerae is the causal organism of the cholera epidemic, which is mostly prevalent in developing and underdeveloped countries. However, incidences of cholera in developed countries are also alarming. Because of the emergence of new drug-resistant strains, even though several generic drugs and vaccines have been developed over time, Vibrio infections remain a global health problem that appeals for the development of novel drugs and vaccines against the pathogen. Here, applying comparative proteomic and reverse vaccinology approaches to the exoproteome and secretome of the pathogen, we have identified three candidate targets (ompU, uppP and yajC) for most of the pathogenic Vibrio strains. Two targets (uppP and yajC) are novel to Vibrio, and two targets (uppP and ompU) can be used to develop both drugs and vaccines (dual targets) against broad spectrum Vibrio serotypes. Using our novel computational approach, we have identified three peptide vaccine candidates that have high potential to induce both B- and T-cell-mediated immune responses from our identified two dual targets. These two targets were modeled and subjected to virtual screening against natural compounds derived from Piper betel. Seven compounds were identified first time from Piper betel to be highly effective to render the function of these targets to identify them as emerging potential drugs against Vibrio. Our preliminary validation suggests that these identified peptide vaccines and betel compounds are highly effective against Vibrio cholerae. Currently we are exhaustively validating these targets, candidate peptide vaccines, and betel derived lead compounds against a number of Vibrio species. |
| first_indexed | 2024-12-11T11:13:22Z |
| format | Article |
| id | doaj.art-10da4422c79445f9ac71eb077fb799f3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T11:13:22Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-10da4422c79445f9ac71eb077fb799f32022-12-22T01:09:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5277310.1371/journal.pone.0052773Exoproteome and secretome derived broad spectrum novel drug and vaccine candidates in Vibrio cholerae targeted by Piper betel derived compounds.Debmalya BarhNeha BarveKrishnakant GuptaSudha ChandraNeha JainSandeep TiwariNidia Leon-SicairosAdrian Canizalez-RomanAnderson Rodrigues dos SantosSyed Shah HassanSíntia AlmeidaRommel Thiago Jucá RamosVinicius Augusto Carvalho de AbreuAdriana Ribeiro CarneiroSiomar de Castro SoaresThiago Luiz de Paula CastroAnderson MiyoshiArtur SilvaAnil KumarAmarendra Narayan MisraKenneth BlumEric R BravermanVasco AzevedoVibrio cholerae is the causal organism of the cholera epidemic, which is mostly prevalent in developing and underdeveloped countries. However, incidences of cholera in developed countries are also alarming. Because of the emergence of new drug-resistant strains, even though several generic drugs and vaccines have been developed over time, Vibrio infections remain a global health problem that appeals for the development of novel drugs and vaccines against the pathogen. Here, applying comparative proteomic and reverse vaccinology approaches to the exoproteome and secretome of the pathogen, we have identified three candidate targets (ompU, uppP and yajC) for most of the pathogenic Vibrio strains. Two targets (uppP and yajC) are novel to Vibrio, and two targets (uppP and ompU) can be used to develop both drugs and vaccines (dual targets) against broad spectrum Vibrio serotypes. Using our novel computational approach, we have identified three peptide vaccine candidates that have high potential to induce both B- and T-cell-mediated immune responses from our identified two dual targets. These two targets were modeled and subjected to virtual screening against natural compounds derived from Piper betel. Seven compounds were identified first time from Piper betel to be highly effective to render the function of these targets to identify them as emerging potential drugs against Vibrio. Our preliminary validation suggests that these identified peptide vaccines and betel compounds are highly effective against Vibrio cholerae. Currently we are exhaustively validating these targets, candidate peptide vaccines, and betel derived lead compounds against a number of Vibrio species.http://europepmc.org/articles/PMC3559646?pdf=render |
| spellingShingle | Debmalya Barh Neha Barve Krishnakant Gupta Sudha Chandra Neha Jain Sandeep Tiwari Nidia Leon-Sicairos Adrian Canizalez-Roman Anderson Rodrigues dos Santos Syed Shah Hassan Síntia Almeida Rommel Thiago Jucá Ramos Vinicius Augusto Carvalho de Abreu Adriana Ribeiro Carneiro Siomar de Castro Soares Thiago Luiz de Paula Castro Anderson Miyoshi Artur Silva Anil Kumar Amarendra Narayan Misra Kenneth Blum Eric R Braverman Vasco Azevedo Exoproteome and secretome derived broad spectrum novel drug and vaccine candidates in Vibrio cholerae targeted by Piper betel derived compounds. PLoS ONE |
| title | Exoproteome and secretome derived broad spectrum novel drug and vaccine candidates in Vibrio cholerae targeted by Piper betel derived compounds. |
| title_full | Exoproteome and secretome derived broad spectrum novel drug and vaccine candidates in Vibrio cholerae targeted by Piper betel derived compounds. |
| title_fullStr | Exoproteome and secretome derived broad spectrum novel drug and vaccine candidates in Vibrio cholerae targeted by Piper betel derived compounds. |
| title_full_unstemmed | Exoproteome and secretome derived broad spectrum novel drug and vaccine candidates in Vibrio cholerae targeted by Piper betel derived compounds. |
| title_short | Exoproteome and secretome derived broad spectrum novel drug and vaccine candidates in Vibrio cholerae targeted by Piper betel derived compounds. |
| title_sort | exoproteome and secretome derived broad spectrum novel drug and vaccine candidates in vibrio cholerae targeted by piper betel derived compounds |
| url | http://europepmc.org/articles/PMC3559646?pdf=render |
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