In Vivo Susceptibility of Plasmodium Vivax to Chloroquine in Southeastern Iran
Background: Plasmodium vivax is the predominant species causes of malaria with about 90% total annual reported malaria in Iran. This study conducted to determine the susceptibility of Plasmodium vivax isolates to chloroquine in Sistan and Balochistan Province, southeastern Iran.Methods: A total 270...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2012-06-01
|
| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/20952.pdf&manuscript_id=20952 |
| _version_ | 1818720997334843392 |
|---|---|
| author | S Dittrich A Raeisi S Shojaee H Keshavarz A Heidari |
| author_facet | S Dittrich A Raeisi S Shojaee H Keshavarz A Heidari |
| author_sort | S Dittrich |
| collection | DOAJ |
| description | Background: Plasmodium vivax is the predominant species causes of malaria with about 90% total annual reported malaria in Iran. This study conducted to determine the susceptibility of Plasmodium vivax isolates to chloroquine in Sistan and Balochistan Province, southeastern Iran.Methods: A total 270 subjects with symptomatic malaria and confirmed P. vivax infection completed the designed 28-day in vivo study. The thick and thin film blood smears were screened for malaria parasites by microscopy. The nested PCR was applied using the Plasmodium 18 subunit ribosomal ribonucleic (Ssr RNA) genes for detecting mixed infections and diagnosis of parasites in the samples with low parasite on days 0, 5, 6, 7, and 28. Results: P. vivax was cleared in 15%, 50%, 95%, and 100% of patients on days 1, 2, 3, 4 respectively by microscopy assessment. Six patients were exhibited specific P. vivax band in nested PCR on day 5. No recurrence was observed on days 7, 14 and 28. Mean (±standard deviation) parasite clearance time was 2.41 (±0.8) days. Conclusion: P. vivax is still susceptible to chloroquine in Southeatern Iran. This finding is compatible with results of neighboring countries Pakistan and Afghanistan. |
| first_indexed | 2024-12-17T20:31:43Z |
| format | Article |
| id | doaj.art-10dd1034b95c4b1a8a8f541f0bee12b0 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-17T20:31:43Z |
| publishDate | 2012-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-10dd1034b95c4b1a8a8f541f0bee12b02022-12-21T21:33:33ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2012-06-0172814In Vivo Susceptibility of Plasmodium Vivax to Chloroquine in Southeastern IranS DittrichA RaeisiS ShojaeeH KeshavarzA HeidariBackground: Plasmodium vivax is the predominant species causes of malaria with about 90% total annual reported malaria in Iran. This study conducted to determine the susceptibility of Plasmodium vivax isolates to chloroquine in Sistan and Balochistan Province, southeastern Iran.Methods: A total 270 subjects with symptomatic malaria and confirmed P. vivax infection completed the designed 28-day in vivo study. The thick and thin film blood smears were screened for malaria parasites by microscopy. The nested PCR was applied using the Plasmodium 18 subunit ribosomal ribonucleic (Ssr RNA) genes for detecting mixed infections and diagnosis of parasites in the samples with low parasite on days 0, 5, 6, 7, and 28. Results: P. vivax was cleared in 15%, 50%, 95%, and 100% of patients on days 1, 2, 3, 4 respectively by microscopy assessment. Six patients were exhibited specific P. vivax band in nested PCR on day 5. No recurrence was observed on days 7, 14 and 28. Mean (±standard deviation) parasite clearance time was 2.41 (±0.8) days. Conclusion: P. vivax is still susceptible to chloroquine in Southeatern Iran. This finding is compatible with results of neighboring countries Pakistan and Afghanistan.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/20952.pdf&manuscript_id=20952MalariaPlasmodium VivaxChloroquineIran |
| spellingShingle | S Dittrich A Raeisi S Shojaee H Keshavarz A Heidari In Vivo Susceptibility of Plasmodium Vivax to Chloroquine in Southeastern Iran Iranian Journal of Parasitology Malaria Plasmodium Vivax Chloroquine Iran |
| title | In Vivo Susceptibility of Plasmodium Vivax to Chloroquine in Southeastern Iran |
| title_full | In Vivo Susceptibility of Plasmodium Vivax to Chloroquine in Southeastern Iran |
| title_fullStr | In Vivo Susceptibility of Plasmodium Vivax to Chloroquine in Southeastern Iran |
| title_full_unstemmed | In Vivo Susceptibility of Plasmodium Vivax to Chloroquine in Southeastern Iran |
| title_short | In Vivo Susceptibility of Plasmodium Vivax to Chloroquine in Southeastern Iran |
| title_sort | in vivo susceptibility of plasmodium vivax to chloroquine in southeastern iran |
| topic | Malaria Plasmodium Vivax Chloroquine Iran |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/20952.pdf&manuscript_id=20952 |
| work_keys_str_mv | AT sdittrich invivosusceptibilityofplasmodiumvivaxtochloroquineinsoutheasterniran AT araeisi invivosusceptibilityofplasmodiumvivaxtochloroquineinsoutheasterniran AT sshojaee invivosusceptibilityofplasmodiumvivaxtochloroquineinsoutheasterniran AT hkeshavarz invivosusceptibilityofplasmodiumvivaxtochloroquineinsoutheasterniran AT aheidari invivosusceptibilityofplasmodiumvivaxtochloroquineinsoutheasterniran |