A Complete Micropropagation Protocol for Black-Leaved <i>Zamioculcas zamiifolia</i> (Lodd.) Engl. ‘Dowon’
<i>Zamioculcas zamiifolia</i>, a drought-resistant plant in the family Araceae, is a popular ornamental potted foliage plant originating from tropical east and subtropical southeast Africa. The growth and propagation rate of this species is low in conventional propagation methods. Theref...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/4/422 |
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| author | Amirhossein Pourhassan Behzad Kaviani Dariusz Kulus Natalia Miler Naser Negahdar |
| author_facet | Amirhossein Pourhassan Behzad Kaviani Dariusz Kulus Natalia Miler Naser Negahdar |
| author_sort | Amirhossein Pourhassan |
| collection | DOAJ |
| description | <i>Zamioculcas zamiifolia</i>, a drought-resistant plant in the family Araceae, is a popular ornamental potted foliage plant originating from tropical east and subtropical southeast Africa. The growth and propagation rate of this species is low in conventional propagation methods. Therefore, the current study aimed at developing a complete in vitro propagation protocol of black-leaved Raven<sup>®</sup> ZZ plant (<i>Z. zamiifolia</i> ‘Dowon’)—a novelty on the floricultural market. In order to initiate an axenic culture, the disinfection of leaf explants was performed with sodium hypochlorite and mercury chloride. Next, leaf segments were cultured on the Murashige and Skoog (MS) medium with the addition of 6-benzyladenine (BA) and/or ɑ-naphthalene acetic acid (NAA) at various concentrations. The highest number of shoots (11) and leaves (22) per explant was obtained in a medium enriched with 2 mg·L<sup>−1</sup> BA together with 0.5 mg·L<sup>−1</sup> NAA. The maximum number of roots (3.33) was produced in microshoots cultured on the medium supplemented with 2 mg·L<sup>−1</sup> NAA. On the other hand, the longest roots (2.66 cm) were produced on a medium containing 2 mg·L<sup>−1</sup> NAA and 0.5 mg·L<sup>−1</sup> BA. The combination of 0.5 mg·L<sup>−1</sup> BA and 0.5 mg·L<sup>−1</sup> NAA was most effective in stimulating callus formation (78.33%). Rooted plantlets were transferred to plastic pots filled with coco peat and acclimatized to ambient greenhouse conditions with an average 68.19% survival rate. This is the first report on a complete micropropagation protocol of black-leaved zamioculcas. |
| first_indexed | 2024-03-11T04:57:43Z |
| format | Article |
| id | doaj.art-10dd26c7c9a54a64bd21291d51c07635 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T04:57:43Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-10dd26c7c9a54a64bd21291d51c076352023-11-17T19:28:29ZengMDPI AGHorticulturae2311-75242023-03-019442210.3390/horticulturae9040422A Complete Micropropagation Protocol for Black-Leaved <i>Zamioculcas zamiifolia</i> (Lodd.) Engl. ‘Dowon’Amirhossein Pourhassan0Behzad Kaviani1Dariusz Kulus2Natalia Miler3Naser Negahdar4Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht 4147654919, IranDepartment of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht 4147654919, IranLaboratory of Ornamental Plants and Vegetable Crops, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, Bernardyńska 6, 85-029 Bydgoszcz, PolandLaboratory of Ornamental Plants and Vegetable Crops, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, Bernardyńska 6, 85-029 Bydgoszcz, PolandDepartment of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht 4147654919, Iran<i>Zamioculcas zamiifolia</i>, a drought-resistant plant in the family Araceae, is a popular ornamental potted foliage plant originating from tropical east and subtropical southeast Africa. The growth and propagation rate of this species is low in conventional propagation methods. Therefore, the current study aimed at developing a complete in vitro propagation protocol of black-leaved Raven<sup>®</sup> ZZ plant (<i>Z. zamiifolia</i> ‘Dowon’)—a novelty on the floricultural market. In order to initiate an axenic culture, the disinfection of leaf explants was performed with sodium hypochlorite and mercury chloride. Next, leaf segments were cultured on the Murashige and Skoog (MS) medium with the addition of 6-benzyladenine (BA) and/or ɑ-naphthalene acetic acid (NAA) at various concentrations. The highest number of shoots (11) and leaves (22) per explant was obtained in a medium enriched with 2 mg·L<sup>−1</sup> BA together with 0.5 mg·L<sup>−1</sup> NAA. The maximum number of roots (3.33) was produced in microshoots cultured on the medium supplemented with 2 mg·L<sup>−1</sup> NAA. On the other hand, the longest roots (2.66 cm) were produced on a medium containing 2 mg·L<sup>−1</sup> NAA and 0.5 mg·L<sup>−1</sup> BA. The combination of 0.5 mg·L<sup>−1</sup> BA and 0.5 mg·L<sup>−1</sup> NAA was most effective in stimulating callus formation (78.33%). Rooted plantlets were transferred to plastic pots filled with coco peat and acclimatized to ambient greenhouse conditions with an average 68.19% survival rate. This is the first report on a complete micropropagation protocol of black-leaved zamioculcas.https://www.mdpi.com/2311-7524/9/4/422Araceaein vitro propagationornamental plantsplant growth regulators |
| spellingShingle | Amirhossein Pourhassan Behzad Kaviani Dariusz Kulus Natalia Miler Naser Negahdar A Complete Micropropagation Protocol for Black-Leaved <i>Zamioculcas zamiifolia</i> (Lodd.) Engl. ‘Dowon’ Horticulturae Araceae in vitro propagation ornamental plants plant growth regulators |
| title | A Complete Micropropagation Protocol for Black-Leaved <i>Zamioculcas zamiifolia</i> (Lodd.) Engl. ‘Dowon’ |
| title_full | A Complete Micropropagation Protocol for Black-Leaved <i>Zamioculcas zamiifolia</i> (Lodd.) Engl. ‘Dowon’ |
| title_fullStr | A Complete Micropropagation Protocol for Black-Leaved <i>Zamioculcas zamiifolia</i> (Lodd.) Engl. ‘Dowon’ |
| title_full_unstemmed | A Complete Micropropagation Protocol for Black-Leaved <i>Zamioculcas zamiifolia</i> (Lodd.) Engl. ‘Dowon’ |
| title_short | A Complete Micropropagation Protocol for Black-Leaved <i>Zamioculcas zamiifolia</i> (Lodd.) Engl. ‘Dowon’ |
| title_sort | complete micropropagation protocol for black leaved i zamioculcas zamiifolia i lodd engl dowon |
| topic | Araceae in vitro propagation ornamental plants plant growth regulators |
| url | https://www.mdpi.com/2311-7524/9/4/422 |
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