Changes in sulfur in soybean rhizosphere soil and the response of microbial flora in a continuous cropping system mediated by Funneliformis mosseae
Soybean is an S-loving crop, and continuous cropping might cause soil sulfur shortage. The primary objectives of this study are to determine whether Funneliformis mosseae (F. mosseae) can enhance the content of available S in S-deficient soil and thereby improve the sulfur utilization rate in soybea...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1235736/full |
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| author | Yizhi Mao Donghao Chang Xiaoying Cui Yunshu Wu Baiyan Cai Baiyan Cai |
| author_facet | Yizhi Mao Donghao Chang Xiaoying Cui Yunshu Wu Baiyan Cai Baiyan Cai |
| author_sort | Yizhi Mao |
| collection | DOAJ |
| description | Soybean is an S-loving crop, and continuous cropping might cause soil sulfur shortage. The primary objectives of this study are to determine whether Funneliformis mosseae (F. mosseae) can enhance the content of available S in S-deficient soil and thereby improve the sulfur utilization rate in soybean. The experiment used Heinong 48 (HN48), a soybean variety with a vast planting area in Heilongjiang Province, and F. mosseae was inoculated in the soil of soybean that had been continuously cropped for 0 and 3 years. The results of the barium sulfur turbidimetric assay show that the sulfur content in the soil and soybean was reduced by continuous cropping and increased by inoculation with F. mosseae; the results of the macro-genome sequencing technology, show that the diversity and abundance of bacteria in the soil was decreased by continuous cropping and increased by inoculation with F. mosseae. The sulfur-oxidizing bacteria (SOB) activity and sulfur-related gene expression levels were lower in the continuous crop group compared to the control group and higher in the F.mosseae-inoculated group compared to the control group. Continuous cropping reduced the sulfur content and ratio of soybean rhizosphere soil, affecting soil flora activity and thus soybean growth; F. mosseae inoculation increased the sulfur content of soybean root-perimeter soil and plants, increased the diversity and abundance of rhizosphere soil microorganisms, increased the expression of genes for sulfur transport systems, sulfur metabolism, and other metabolic functions related to elemental sulfur, and increased the species abundance and metabolic vigor of most SOB. In summary, continuous cropping inhibits soil sulfur uptake and utilization in soybean while the inoculation with F. mosseae can significantly improve this situation. This study offers a theoretical research foundation for using AMF as a bio-fungal agent to enhance soil sulfur use. It also supports the decrease of chemical fertilizers, their substitution, and the protection of native soil. |
| first_indexed | 2024-03-12T13:39:54Z |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T13:39:54Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-10de74a2f5134f658b44c85124dec37d2023-08-23T19:17:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12357361235736Changes in sulfur in soybean rhizosphere soil and the response of microbial flora in a continuous cropping system mediated by Funneliformis mosseaeYizhi Mao0Donghao Chang1Xiaoying Cui2Yunshu Wu3Baiyan Cai4Baiyan Cai5Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education and Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region and Key Laboratory of Molecular Biology, College of Heilongjiang Province and School of Life Sciences, Heilongjiang University, Harbin, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education and Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region and Key Laboratory of Molecular Biology, College of Heilongjiang Province and School of Life Sciences, Heilongjiang University, Harbin, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education and Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region and Key Laboratory of Molecular Biology, College of Heilongjiang Province and School of Life Sciences, Heilongjiang University, Harbin, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education and Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region and Key Laboratory of Molecular Biology, College of Heilongjiang Province and School of Life Sciences, Heilongjiang University, Harbin, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education and Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region and Key Laboratory of Molecular Biology, College of Heilongjiang Province and School of Life Sciences, Heilongjiang University, Harbin, ChinaHebei Key Laboratory of Agroecological Safety, Hebei University of Environmental Engineering, Qinhuangdao, ChinaSoybean is an S-loving crop, and continuous cropping might cause soil sulfur shortage. The primary objectives of this study are to determine whether Funneliformis mosseae (F. mosseae) can enhance the content of available S in S-deficient soil and thereby improve the sulfur utilization rate in soybean. The experiment used Heinong 48 (HN48), a soybean variety with a vast planting area in Heilongjiang Province, and F. mosseae was inoculated in the soil of soybean that had been continuously cropped for 0 and 3 years. The results of the barium sulfur turbidimetric assay show that the sulfur content in the soil and soybean was reduced by continuous cropping and increased by inoculation with F. mosseae; the results of the macro-genome sequencing technology, show that the diversity and abundance of bacteria in the soil was decreased by continuous cropping and increased by inoculation with F. mosseae. The sulfur-oxidizing bacteria (SOB) activity and sulfur-related gene expression levels were lower in the continuous crop group compared to the control group and higher in the F.mosseae-inoculated group compared to the control group. Continuous cropping reduced the sulfur content and ratio of soybean rhizosphere soil, affecting soil flora activity and thus soybean growth; F. mosseae inoculation increased the sulfur content of soybean root-perimeter soil and plants, increased the diversity and abundance of rhizosphere soil microorganisms, increased the expression of genes for sulfur transport systems, sulfur metabolism, and other metabolic functions related to elemental sulfur, and increased the species abundance and metabolic vigor of most SOB. In summary, continuous cropping inhibits soil sulfur uptake and utilization in soybean while the inoculation with F. mosseae can significantly improve this situation. This study offers a theoretical research foundation for using AMF as a bio-fungal agent to enhance soil sulfur use. It also supports the decrease of chemical fertilizers, their substitution, and the protection of native soil.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1235736/fullsoybeancontinuous cropping soilFunneliformis Mosseaesulfurresponse of florasulfur functional gene |
| spellingShingle | Yizhi Mao Donghao Chang Xiaoying Cui Yunshu Wu Baiyan Cai Baiyan Cai Changes in sulfur in soybean rhizosphere soil and the response of microbial flora in a continuous cropping system mediated by Funneliformis mosseae Frontiers in Microbiology soybean continuous cropping soil Funneliformis Mosseae sulfur response of flora sulfur functional gene |
| title | Changes in sulfur in soybean rhizosphere soil and the response of microbial flora in a continuous cropping system mediated by Funneliformis mosseae |
| title_full | Changes in sulfur in soybean rhizosphere soil and the response of microbial flora in a continuous cropping system mediated by Funneliformis mosseae |
| title_fullStr | Changes in sulfur in soybean rhizosphere soil and the response of microbial flora in a continuous cropping system mediated by Funneliformis mosseae |
| title_full_unstemmed | Changes in sulfur in soybean rhizosphere soil and the response of microbial flora in a continuous cropping system mediated by Funneliformis mosseae |
| title_short | Changes in sulfur in soybean rhizosphere soil and the response of microbial flora in a continuous cropping system mediated by Funneliformis mosseae |
| title_sort | changes in sulfur in soybean rhizosphere soil and the response of microbial flora in a continuous cropping system mediated by funneliformis mosseae |
| topic | soybean continuous cropping soil Funneliformis Mosseae sulfur response of flora sulfur functional gene |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1235736/full |
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