Non-Invasive Probing of Winter Dormancy via Time-Frequency Analysis of Induced Chlorophyll Fluorescence in Deciduous Plants as Exemplified by Apple (<i>Malus</i> × <i>domestica</i> Borkh.)
Dormancy is a physiological state that confers winter hardiness to and orchestrates phenological phase progression in temperate perennial plants. Weather fluctuations caused by climate change increasingly disturb dormancy onset and release in plants including tree crops, causing aberrant growth, flo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/21/2811 |
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| author | Boris Shurygin Ivan Konyukhov Sergei Khruschev Alexei Solovchenko |
| author_facet | Boris Shurygin Ivan Konyukhov Sergei Khruschev Alexei Solovchenko |
| author_sort | Boris Shurygin |
| collection | DOAJ |
| description | Dormancy is a physiological state that confers winter hardiness to and orchestrates phenological phase progression in temperate perennial plants. Weather fluctuations caused by climate change increasingly disturb dormancy onset and release in plants including tree crops, causing aberrant growth, flowering and fruiting. Research in this field suffers from the lack of affordable non-invasive methods for online dormancy monitoring. We propose an automatic framework for low-cost, long-term, scalable dormancy studies in deciduous plants. It is based on continuous sensing of the photosynthetic activity of shoots via pulse-amplitude-modulated chlorophyll fluorescence sensors connected remotely to a data processing system. The resulting high-resolution time series of JIP-test parameters indicative of the responsiveness of the photosynthetic apparatus to environmental stimuli were subjected to frequency-domain analysis. The proposed approach overcomes the variance coming from diurnal changes of insolation and provides hints on the depth of dormancy. Our approach was validated over three seasons in an apple (<i>Malus × domestica</i> Borkh.) orchard by collating the non-invasive estimations with the results of traditional methods (growing of the cuttings obtained from the trees at different phases of dormancy) and the output of chilling requirement models. We discuss the advantages of the proposed monitoring framework such as prompt detection of frost damage along with its potential limitations. |
| first_indexed | 2024-03-09T18:43:44Z |
| format | Article |
| id | doaj.art-10deb138dea543d695eec06f42ac45b1 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T18:43:44Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-10deb138dea543d695eec06f42ac45b12023-11-24T06:23:26ZengMDPI AGPlants2223-77472022-10-011121281110.3390/plants11212811Non-Invasive Probing of Winter Dormancy via Time-Frequency Analysis of Induced Chlorophyll Fluorescence in Deciduous Plants as Exemplified by Apple (<i>Malus</i> × <i>domestica</i> Borkh.)Boris Shurygin0Ivan Konyukhov1Sergei Khruschev2Alexei Solovchenko3Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory 1/12, 119234 Moscow, RussiaFaculty of Biology, Lomonosov Moscow State University, Leninskie Gory 1/12, 119234 Moscow, RussiaFaculty of Biology, Lomonosov Moscow State University, Leninskie Gory 1/12, 119234 Moscow, RussiaFaculty of Biology, Lomonosov Moscow State University, Leninskie Gory 1/12, 119234 Moscow, RussiaDormancy is a physiological state that confers winter hardiness to and orchestrates phenological phase progression in temperate perennial plants. Weather fluctuations caused by climate change increasingly disturb dormancy onset and release in plants including tree crops, causing aberrant growth, flowering and fruiting. Research in this field suffers from the lack of affordable non-invasive methods for online dormancy monitoring. We propose an automatic framework for low-cost, long-term, scalable dormancy studies in deciduous plants. It is based on continuous sensing of the photosynthetic activity of shoots via pulse-amplitude-modulated chlorophyll fluorescence sensors connected remotely to a data processing system. The resulting high-resolution time series of JIP-test parameters indicative of the responsiveness of the photosynthetic apparatus to environmental stimuli were subjected to frequency-domain analysis. The proposed approach overcomes the variance coming from diurnal changes of insolation and provides hints on the depth of dormancy. Our approach was validated over three seasons in an apple (<i>Malus × domestica</i> Borkh.) orchard by collating the non-invasive estimations with the results of traditional methods (growing of the cuttings obtained from the trees at different phases of dormancy) and the output of chilling requirement models. We discuss the advantages of the proposed monitoring framework such as prompt detection of frost damage along with its potential limitations.https://www.mdpi.com/2223-7747/11/21/2811chilling requirementchlorophyll fluorescencenon-photochemical quenchingPAMphotoprotectionstress resilience |
| spellingShingle | Boris Shurygin Ivan Konyukhov Sergei Khruschev Alexei Solovchenko Non-Invasive Probing of Winter Dormancy via Time-Frequency Analysis of Induced Chlorophyll Fluorescence in Deciduous Plants as Exemplified by Apple (<i>Malus</i> × <i>domestica</i> Borkh.) Plants chilling requirement chlorophyll fluorescence non-photochemical quenching PAM photoprotection stress resilience |
| title | Non-Invasive Probing of Winter Dormancy via Time-Frequency Analysis of Induced Chlorophyll Fluorescence in Deciduous Plants as Exemplified by Apple (<i>Malus</i> × <i>domestica</i> Borkh.) |
| title_full | Non-Invasive Probing of Winter Dormancy via Time-Frequency Analysis of Induced Chlorophyll Fluorescence in Deciduous Plants as Exemplified by Apple (<i>Malus</i> × <i>domestica</i> Borkh.) |
| title_fullStr | Non-Invasive Probing of Winter Dormancy via Time-Frequency Analysis of Induced Chlorophyll Fluorescence in Deciduous Plants as Exemplified by Apple (<i>Malus</i> × <i>domestica</i> Borkh.) |
| title_full_unstemmed | Non-Invasive Probing of Winter Dormancy via Time-Frequency Analysis of Induced Chlorophyll Fluorescence in Deciduous Plants as Exemplified by Apple (<i>Malus</i> × <i>domestica</i> Borkh.) |
| title_short | Non-Invasive Probing of Winter Dormancy via Time-Frequency Analysis of Induced Chlorophyll Fluorescence in Deciduous Plants as Exemplified by Apple (<i>Malus</i> × <i>domestica</i> Borkh.) |
| title_sort | non invasive probing of winter dormancy via time frequency analysis of induced chlorophyll fluorescence in deciduous plants as exemplified by apple i malus i i domestica i borkh |
| topic | chilling requirement chlorophyll fluorescence non-photochemical quenching PAM photoprotection stress resilience |
| url | https://www.mdpi.com/2223-7747/11/21/2811 |
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