Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1
Intelectins are putative innate immune lectins that are found throughout chordates. The first intelectin reported was Xenopus laevis cortical granule lectin-1 (XCGL-1 or XL-35). XCGL-1 is critical in fertilization membrane development in Xenopus. Here, we explored the biochemical properties of XCGL-...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402201684X |
| _version_ | 1811188126735925248 |
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| author | Peerapon Deetanya Thassanai Sitthiyotha Nusara Chomanee Surasak Chunsrivirot Kittikhun Wangkanont |
| author_facet | Peerapon Deetanya Thassanai Sitthiyotha Nusara Chomanee Surasak Chunsrivirot Kittikhun Wangkanont |
| author_sort | Peerapon Deetanya |
| collection | DOAJ |
| description | Intelectins are putative innate immune lectins that are found throughout chordates. The first intelectin reported was Xenopus laevis cortical granule lectin-1 (XCGL-1 or XL-35). XCGL-1 is critical in fertilization membrane development in Xenopus. Here, we explored the biochemical properties of XCGL-1. The cysteines responsible for forming intermolecular disulfide bonds were identified. XCGL-1 adopted a four-lobed structure as observed by electron microscopy. The full-length XCGL-1 and the carbohydrate recognition domain (CRD) bind galactose-containing carbohydrates at nanomolar to micromolar affinities. Molecular modeling suggested that galactoside ligands coordinated the binding site calcium ion and interacted with residues around the groove made available by the non-conserved substitution compared to human intelectin-1. Folding conditions for production of recombinant XCGL-1 CRD were also investigated. Our results not only provide new biochemical insights into the function of XCGL-1, but may also provide foundation for further applications of XCGL-1 as glycobiology tools. |
| first_indexed | 2024-04-11T14:15:21Z |
| format | Article |
| id | doaj.art-10e0365e1eca40969a5b3f9e002c53c6 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-11T14:15:21Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-10e0365e1eca40969a5b3f9e002c53c62022-12-22T04:19:33ZengElsevierHeliyon2405-84402022-08-0188e10396Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1Peerapon Deetanya0Thassanai Sitthiyotha1Nusara Chomanee2Surasak Chunsrivirot3Kittikhun Wangkanont4Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence for Molecular Crop, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, ThailandStructural and Computational Biology Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, ThailandElectron Microscopy Unit, Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, ThailandStructural and Computational Biology Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, ThailandCenter of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence for Molecular Crop, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Corresponding author.Intelectins are putative innate immune lectins that are found throughout chordates. The first intelectin reported was Xenopus laevis cortical granule lectin-1 (XCGL-1 or XL-35). XCGL-1 is critical in fertilization membrane development in Xenopus. Here, we explored the biochemical properties of XCGL-1. The cysteines responsible for forming intermolecular disulfide bonds were identified. XCGL-1 adopted a four-lobed structure as observed by electron microscopy. The full-length XCGL-1 and the carbohydrate recognition domain (CRD) bind galactose-containing carbohydrates at nanomolar to micromolar affinities. Molecular modeling suggested that galactoside ligands coordinated the binding site calcium ion and interacted with residues around the groove made available by the non-conserved substitution compared to human intelectin-1. Folding conditions for production of recombinant XCGL-1 CRD were also investigated. Our results not only provide new biochemical insights into the function of XCGL-1, but may also provide foundation for further applications of XCGL-1 as glycobiology tools.http://www.sciencedirect.com/science/article/pii/S240584402201684XXenopus laevis cortical granule lectin-1XCGL-1XL35IntelectinOmentin |
| spellingShingle | Peerapon Deetanya Thassanai Sitthiyotha Nusara Chomanee Surasak Chunsrivirot Kittikhun Wangkanont Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1 Heliyon Xenopus laevis cortical granule lectin-1 XCGL-1 XL35 Intelectin Omentin |
| title | Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1 |
| title_full | Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1 |
| title_fullStr | Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1 |
| title_full_unstemmed | Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1 |
| title_short | Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1 |
| title_sort | biochemical and ligand binding properties of recombinant xenopus laevis cortical granule lectin 1 |
| topic | Xenopus laevis cortical granule lectin-1 XCGL-1 XL35 Intelectin Omentin |
| url | http://www.sciencedirect.com/science/article/pii/S240584402201684X |
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