Circulating Genetically Abnormal Cells Add Non-Invasive Diagnosis Value to Discriminate Lung Cancer in Patients With Pulmonary Nodules ≤10 mm
BackgroundLung cancer screening using low-dose computed tomography (LDCT) often leads to unnecessary biopsy because of the low specificity among patients with pulmonary nodules ≤10 mm. Circulating genetically abnormal cells (CACs) can be used to discriminate lung cancer from benign lung disease. To...
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| Format: | Article |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.638223/full |
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| author | Maosong Ye Xiaoxuan Zheng Xiaoxuan Zheng Xin Ye Xin Ye Xin Ye Juncheng Zhang Juncheng Zhang Chuoji Huang Chuoji Huang Zilong Liu Meng Huang Meng Huang Xianjun Fan Xianjun Fan Yanci Chen Yanci Chen Botao Xiao Jiayuan Sun Jiayuan Sun Chunxue Bai |
| author_facet | Maosong Ye Xiaoxuan Zheng Xiaoxuan Zheng Xin Ye Xin Ye Xin Ye Juncheng Zhang Juncheng Zhang Chuoji Huang Chuoji Huang Zilong Liu Meng Huang Meng Huang Xianjun Fan Xianjun Fan Yanci Chen Yanci Chen Botao Xiao Jiayuan Sun Jiayuan Sun Chunxue Bai |
| author_sort | Maosong Ye |
| collection | DOAJ |
| description | BackgroundLung cancer screening using low-dose computed tomography (LDCT) often leads to unnecessary biopsy because of the low specificity among patients with pulmonary nodules ≤10 mm. Circulating genetically abnormal cells (CACs) can be used to discriminate lung cancer from benign lung disease. To examine the diagnostic value of CACs in detecting lung cancer for patients with malignant pulmonary nodules ≤10 mm.MethodsIn this prospective study, patients with pulmonary nodules ≤10 mm who were detected at four hospitals in China from January 2019 to January 2020 were included. CACs were detected using fluorescence in-situ hybridization. All patients were confirmed as lung cancer or benign disease by further histopathological examination. Multivariable logistic regression models were established to detect the presence of lung cancer using CACs and other associated characteristics. Receiver operating characteristic analysis was used to evaluate the performance of CACs for lung cancer diagnosis.ResultsOverall, 125 patients were included and analyzed. When the cutoff value of CACs was >2, the sensitivity and specificity for lung cancer were 70.5 and 86.4%. Male (OR = 0.330, P = 0.005), maximum solid nodule (OR = 2.362, P = 0.089), maximum nodule located in upper lobe (OR = 3.867, P = 0.001), and CACs >2 (OR = 18.525, P < 0.001) met the P < 0.10 criterion for inclusion in the multivariable models. The multivariable logistic regression model that included the dichotomized CACs (>2 vs. ≤2) and other clinical factors (AUC = 0.907, 95% CI = 0.842–0.951) was superior to the models that only considered dichotomized CACs or other clinical factors and similar to the model with numerical CACs and other clinical factors (AUC = 0.913, 95% CI = 0.850–0.956).ConclusionCACs presented a significant diagnostic value in detecting lung cancer for patients with pulmonary nodules ≤10 mm. |
| first_indexed | 2024-12-19T23:51:57Z |
| format | Article |
| id | doaj.art-10e13817ffa74410b2537c329f896668 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-19T23:51:57Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Oncology |
| spelling | doaj.art-10e13817ffa74410b2537c329f8966682022-12-21T20:01:06ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-03-011110.3389/fonc.2021.638223638223Circulating Genetically Abnormal Cells Add Non-Invasive Diagnosis Value to Discriminate Lung Cancer in Patients With Pulmonary Nodules ≤10 mmMaosong Ye0Xiaoxuan Zheng1Xiaoxuan Zheng2Xin Ye3Xin Ye4Xin Ye5Juncheng Zhang6Juncheng Zhang7Chuoji Huang8Chuoji Huang9Zilong Liu10Meng Huang11Meng Huang12Xianjun Fan13Xianjun Fan14Yanci Chen15Yanci Chen16Botao Xiao17Jiayuan Sun18Jiayuan Sun19Chunxue Bai20Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Respiratory Endoscopy, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Respiratory and Critical Care Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, ChinaJoint Research Center of Liquid Biopsy in Guangdong, Hong Kong, and Macao, Zhuhai, ChinaZhuhai Sanmed Biotech Ltd., Zhuhai, ChinaSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, ChinaJoint Research Center of Liquid Biopsy in Guangdong, Hong Kong, and Macao, Zhuhai, ChinaZhuhai Sanmed Biotech Ltd., Zhuhai, ChinaJoint Research Center of Liquid Biopsy in Guangdong, Hong Kong, and Macao, Zhuhai, ChinaZhuhai Sanmed Biotech Ltd., Zhuhai, ChinaDepartment of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, ChinaJoint Research Center of Liquid Biopsy in Guangdong, Hong Kong, and Macao, Zhuhai, ChinaZhuhai Sanmed Biotech Ltd., Zhuhai, ChinaJoint Research Center of Liquid Biopsy in Guangdong, Hong Kong, and Macao, Zhuhai, ChinaZhuhai Sanmed Biotech Ltd., Zhuhai, ChinaJoint Research Center of Liquid Biopsy in Guangdong, Hong Kong, and Macao, Zhuhai, ChinaZhuhai Sanmed Biotech Ltd., Zhuhai, ChinaSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, ChinaDepartment of Respiratory Endoscopy, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Respiratory and Critical Care Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, ChinaBackgroundLung cancer screening using low-dose computed tomography (LDCT) often leads to unnecessary biopsy because of the low specificity among patients with pulmonary nodules ≤10 mm. Circulating genetically abnormal cells (CACs) can be used to discriminate lung cancer from benign lung disease. To examine the diagnostic value of CACs in detecting lung cancer for patients with malignant pulmonary nodules ≤10 mm.MethodsIn this prospective study, patients with pulmonary nodules ≤10 mm who were detected at four hospitals in China from January 2019 to January 2020 were included. CACs were detected using fluorescence in-situ hybridization. All patients were confirmed as lung cancer or benign disease by further histopathological examination. Multivariable logistic regression models were established to detect the presence of lung cancer using CACs and other associated characteristics. Receiver operating characteristic analysis was used to evaluate the performance of CACs for lung cancer diagnosis.ResultsOverall, 125 patients were included and analyzed. When the cutoff value of CACs was >2, the sensitivity and specificity for lung cancer were 70.5 and 86.4%. Male (OR = 0.330, P = 0.005), maximum solid nodule (OR = 2.362, P = 0.089), maximum nodule located in upper lobe (OR = 3.867, P = 0.001), and CACs >2 (OR = 18.525, P < 0.001) met the P < 0.10 criterion for inclusion in the multivariable models. The multivariable logistic regression model that included the dichotomized CACs (>2 vs. ≤2) and other clinical factors (AUC = 0.907, 95% CI = 0.842–0.951) was superior to the models that only considered dichotomized CACs or other clinical factors and similar to the model with numerical CACs and other clinical factors (AUC = 0.913, 95% CI = 0.850–0.956).ConclusionCACs presented a significant diagnostic value in detecting lung cancer for patients with pulmonary nodules ≤10 mm.https://www.frontiersin.org/articles/10.3389/fonc.2021.638223/fulllung cancerpulmonary noduleearly detectionearly diagnosiscirculating genetically abnormal cell (CAC) |
| spellingShingle | Maosong Ye Xiaoxuan Zheng Xiaoxuan Zheng Xin Ye Xin Ye Xin Ye Juncheng Zhang Juncheng Zhang Chuoji Huang Chuoji Huang Zilong Liu Meng Huang Meng Huang Xianjun Fan Xianjun Fan Yanci Chen Yanci Chen Botao Xiao Jiayuan Sun Jiayuan Sun Chunxue Bai Circulating Genetically Abnormal Cells Add Non-Invasive Diagnosis Value to Discriminate Lung Cancer in Patients With Pulmonary Nodules ≤10 mm Frontiers in Oncology lung cancer pulmonary nodule early detection early diagnosis circulating genetically abnormal cell (CAC) |
| title | Circulating Genetically Abnormal Cells Add Non-Invasive Diagnosis Value to Discriminate Lung Cancer in Patients With Pulmonary Nodules ≤10 mm |
| title_full | Circulating Genetically Abnormal Cells Add Non-Invasive Diagnosis Value to Discriminate Lung Cancer in Patients With Pulmonary Nodules ≤10 mm |
| title_fullStr | Circulating Genetically Abnormal Cells Add Non-Invasive Diagnosis Value to Discriminate Lung Cancer in Patients With Pulmonary Nodules ≤10 mm |
| title_full_unstemmed | Circulating Genetically Abnormal Cells Add Non-Invasive Diagnosis Value to Discriminate Lung Cancer in Patients With Pulmonary Nodules ≤10 mm |
| title_short | Circulating Genetically Abnormal Cells Add Non-Invasive Diagnosis Value to Discriminate Lung Cancer in Patients With Pulmonary Nodules ≤10 mm |
| title_sort | circulating genetically abnormal cells add non invasive diagnosis value to discriminate lung cancer in patients with pulmonary nodules ≤10 mm |
| topic | lung cancer pulmonary nodule early detection early diagnosis circulating genetically abnormal cell (CAC) |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.638223/full |
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