Sleep EEG in young people with 22q11.2 deletion syndrome: A cross-sectional study of slow-waves, spindles and correlations with memory and neurodevelopmental symptoms
Background: Young people living with 22q11.2 Deletion Syndrome (22q11.2DS) are at increased risk of schizophrenia, intellectual disability, attention-deficit hyperactivity disorder (ADHD) and autism spectrum disorder (ASD). In common with these conditions, 22q11.2DS is also associated with sleep pro...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2022-08-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/75482 |
| _version_ | 1811181648256958464 |
|---|---|
| author | Nicholas A Donnelly Ullrich Bartsch Hayley A Moulding Christopher Eaton Hugh Marston Jessica H Hall Jeremy Hall Michael J Owen Marianne BM van den Bree Matt W Jones |
| author_facet | Nicholas A Donnelly Ullrich Bartsch Hayley A Moulding Christopher Eaton Hugh Marston Jessica H Hall Jeremy Hall Michael J Owen Marianne BM van den Bree Matt W Jones |
| author_sort | Nicholas A Donnelly |
| collection | DOAJ |
| description | Background: Young people living with 22q11.2 Deletion Syndrome (22q11.2DS) are at increased risk of schizophrenia, intellectual disability, attention-deficit hyperactivity disorder (ADHD) and autism spectrum disorder (ASD). In common with these conditions, 22q11.2DS is also associated with sleep problems. We investigated whether abnormal sleep or sleep-dependent network activity in 22q11.2DS reflects convergent, early signatures of neural circuit disruption also evident in associated neurodevelopmental conditions.
Methods: In a cross-sectional design, we recorded high-density sleep EEG in young people (6–20 years) with 22q11.2DS (n=28) and their unaffected siblings (n=17), quantifying associations between sleep architecture, EEG oscillations (spindles and slow waves) and psychiatric symptoms. We also measured performance on a memory task before and after sleep.
Results: 22q11.2DS was associated with significant alterations in sleep architecture, including a greater proportion of N3 sleep and lower proportions of N1 and REM sleep than in siblings. During sleep, deletion carriers showed broadband increases in EEG power with increased slow-wave and spindle amplitudes, increased spindle frequency and density, and stronger coupling between spindles and slow-waves. Spindle and slow-wave amplitudes correlated positively with overnight memory in controls, but negatively in 22q11.2DS. Mediation analyses indicated that genotype effects on anxiety, ADHD and ASD were partially mediated by sleep EEG measures.
Conclusions: This study provides a detailed description of sleep neurophysiology in 22q11.2DS, highlighting alterations in EEG signatures of sleep which have been previously linked to neurodevelopment, some of which were associated with psychiatric symptoms. Sleep EEG features may therefore reflect delayed or compromised neurodevelopmental processes in 22q11.2DS, which could inform our understanding of the neurobiology of this condition and be biomarkers for neuropsychiatric disorders.
Funding: This research was funded by a Lilly Innovation Fellowship Award (UB), the National Institute of Mental Health (NIMH 5UO1MH101724; MvdB), a Wellcome Trust Institutional Strategic Support Fund (ISSF) award (MvdB), the Waterloo Foundation (918-1234; MvdB), the Baily Thomas Charitable Fund (2315/1; MvdB), MRC grant Intellectual Disability and Mental Health: Assessing Genomic Impact on Neurodevelopment (IMAGINE) (MR/L011166/1; JH, MvdB and MO), MRC grant Intellectual Disability and Mental Health: Assessing Genomic Impact on Neurodevelopment 2 (IMAGINE-2) (MR/T033045/1; MvdB, JH and MO); Wellcome Trust Strategic Award ‘Defining Endophenotypes From Integrated Neurosciences’ Wellcome Trust (100202/Z/12/Z MO, JH). NAD was supported by a National Institute for Health Research Academic Clinical Fellowship in Mental Health and MWJ by a Wellcome Trust Senior Research Fellowship in Basic Biomedical Science (202810/Z/16/Z). CE and HAM were supported by Medical Research Council Doctoral Training Grants (C.B.E. 1644194, H.A.M MR/K501347/1). HMM and UB were employed by Eli Lilly & Co during the study; HMM is currently an employee of Boehringer Ingelheim Pharma GmbH & Co KG. The views and opinions expressed are those of the author(s), and not necessarily those of the NHS, the NIHR or the Department of Health funders. |
| first_indexed | 2024-04-11T09:21:04Z |
| format | Article |
| id | doaj.art-10e17dd171704029965f897bf2879c84 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:21:04Z |
| publishDate | 2022-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-10e17dd171704029965f897bf2879c842022-12-22T04:32:12ZengeLife Sciences Publications LtdeLife2050-084X2022-08-011110.7554/eLife.75482Sleep EEG in young people with 22q11.2 deletion syndrome: A cross-sectional study of slow-waves, spindles and correlations with memory and neurodevelopmental symptomsNicholas A Donnelly0https://orcid.org/0000-0003-2234-8545Ullrich Bartsch1https://orcid.org/0000-0002-2213-8989Hayley A Moulding2Christopher Eaton3https://orcid.org/0000-0001-6739-1999Hugh Marston4Jessica H Hall5Jeremy Hall6Michael J Owen7Marianne BM van den Bree8Matt W Jones9Centre for Academic Mental Health, University of Bristol, Bristol, United Kingdom; Avon and Wiltshire Partnership NHS Mental Health Trust, Avon, United KingdomSchool of Physiology, Pharmacology and Neuroscience, University of Bristol, Bristol, United Kingdom; Translational Neuroscience, Eli Lilly, Windlesham, United StatesMedical Research Council Centre for Neuropsychiatric Genetics and Genomics, Cardiff University, Cardiff, United KingdomMedical Research Council Centre for Neuropsychiatric Genetics and Genomics, Cardiff University, Cardiff, United KingdomTranslational Neuroscience, Eli Lilly, Windlesham, United StatesMedical Research Council Centre for Neuropsychiatric Genetics and Genomics, Cardiff University, Cardiff, United KingdomMedical Research Council Centre for Neuropsychiatric Genetics and Genomics, Cardiff University, Cardiff, United KingdomMedical Research Council Centre for Neuropsychiatric Genetics and Genomics, Cardiff University, Cardiff, United KingdomMedical Research Council Centre for Neuropsychiatric Genetics and Genomics, Cardiff University, Cardiff, United KingdomUniversity of Bristol, Bristol, United KingdomBackground: Young people living with 22q11.2 Deletion Syndrome (22q11.2DS) are at increased risk of schizophrenia, intellectual disability, attention-deficit hyperactivity disorder (ADHD) and autism spectrum disorder (ASD). In common with these conditions, 22q11.2DS is also associated with sleep problems. We investigated whether abnormal sleep or sleep-dependent network activity in 22q11.2DS reflects convergent, early signatures of neural circuit disruption also evident in associated neurodevelopmental conditions. Methods: In a cross-sectional design, we recorded high-density sleep EEG in young people (6–20 years) with 22q11.2DS (n=28) and their unaffected siblings (n=17), quantifying associations between sleep architecture, EEG oscillations (spindles and slow waves) and psychiatric symptoms. We also measured performance on a memory task before and after sleep. Results: 22q11.2DS was associated with significant alterations in sleep architecture, including a greater proportion of N3 sleep and lower proportions of N1 and REM sleep than in siblings. During sleep, deletion carriers showed broadband increases in EEG power with increased slow-wave and spindle amplitudes, increased spindle frequency and density, and stronger coupling between spindles and slow-waves. Spindle and slow-wave amplitudes correlated positively with overnight memory in controls, but negatively in 22q11.2DS. Mediation analyses indicated that genotype effects on anxiety, ADHD and ASD were partially mediated by sleep EEG measures. Conclusions: This study provides a detailed description of sleep neurophysiology in 22q11.2DS, highlighting alterations in EEG signatures of sleep which have been previously linked to neurodevelopment, some of which were associated with psychiatric symptoms. Sleep EEG features may therefore reflect delayed or compromised neurodevelopmental processes in 22q11.2DS, which could inform our understanding of the neurobiology of this condition and be biomarkers for neuropsychiatric disorders. Funding: This research was funded by a Lilly Innovation Fellowship Award (UB), the National Institute of Mental Health (NIMH 5UO1MH101724; MvdB), a Wellcome Trust Institutional Strategic Support Fund (ISSF) award (MvdB), the Waterloo Foundation (918-1234; MvdB), the Baily Thomas Charitable Fund (2315/1; MvdB), MRC grant Intellectual Disability and Mental Health: Assessing Genomic Impact on Neurodevelopment (IMAGINE) (MR/L011166/1; JH, MvdB and MO), MRC grant Intellectual Disability and Mental Health: Assessing Genomic Impact on Neurodevelopment 2 (IMAGINE-2) (MR/T033045/1; MvdB, JH and MO); Wellcome Trust Strategic Award ‘Defining Endophenotypes From Integrated Neurosciences’ Wellcome Trust (100202/Z/12/Z MO, JH). NAD was supported by a National Institute for Health Research Academic Clinical Fellowship in Mental Health and MWJ by a Wellcome Trust Senior Research Fellowship in Basic Biomedical Science (202810/Z/16/Z). CE and HAM were supported by Medical Research Council Doctoral Training Grants (C.B.E. 1644194, H.A.M MR/K501347/1). HMM and UB were employed by Eli Lilly & Co during the study; HMM is currently an employee of Boehringer Ingelheim Pharma GmbH & Co KG. The views and opinions expressed are those of the author(s), and not necessarily those of the NHS, the NIHR or the Department of Health funders.https://elifesciences.org/articles/7548222q11.2 deletion syndromeneurodevelopmental disorderspsychosissleepEEGbiomarkers |
| spellingShingle | Nicholas A Donnelly Ullrich Bartsch Hayley A Moulding Christopher Eaton Hugh Marston Jessica H Hall Jeremy Hall Michael J Owen Marianne BM van den Bree Matt W Jones Sleep EEG in young people with 22q11.2 deletion syndrome: A cross-sectional study of slow-waves, spindles and correlations with memory and neurodevelopmental symptoms eLife 22q11.2 deletion syndrome neurodevelopmental disorders psychosis sleep EEG biomarkers |
| title | Sleep EEG in young people with 22q11.2 deletion syndrome: A cross-sectional study of slow-waves, spindles and correlations with memory and neurodevelopmental symptoms |
| title_full | Sleep EEG in young people with 22q11.2 deletion syndrome: A cross-sectional study of slow-waves, spindles and correlations with memory and neurodevelopmental symptoms |
| title_fullStr | Sleep EEG in young people with 22q11.2 deletion syndrome: A cross-sectional study of slow-waves, spindles and correlations with memory and neurodevelopmental symptoms |
| title_full_unstemmed | Sleep EEG in young people with 22q11.2 deletion syndrome: A cross-sectional study of slow-waves, spindles and correlations with memory and neurodevelopmental symptoms |
| title_short | Sleep EEG in young people with 22q11.2 deletion syndrome: A cross-sectional study of slow-waves, spindles and correlations with memory and neurodevelopmental symptoms |
| title_sort | sleep eeg in young people with 22q11 2 deletion syndrome a cross sectional study of slow waves spindles and correlations with memory and neurodevelopmental symptoms |
| topic | 22q11.2 deletion syndrome neurodevelopmental disorders psychosis sleep EEG biomarkers |
| url | https://elifesciences.org/articles/75482 |
| work_keys_str_mv | AT nicholasadonnelly sleepeeginyoungpeoplewith22q112deletionsyndromeacrosssectionalstudyofslowwavesspindlesandcorrelationswithmemoryandneurodevelopmentalsymptoms AT ullrichbartsch sleepeeginyoungpeoplewith22q112deletionsyndromeacrosssectionalstudyofslowwavesspindlesandcorrelationswithmemoryandneurodevelopmentalsymptoms AT hayleyamoulding sleepeeginyoungpeoplewith22q112deletionsyndromeacrosssectionalstudyofslowwavesspindlesandcorrelationswithmemoryandneurodevelopmentalsymptoms AT christophereaton sleepeeginyoungpeoplewith22q112deletionsyndromeacrosssectionalstudyofslowwavesspindlesandcorrelationswithmemoryandneurodevelopmentalsymptoms AT hughmarston sleepeeginyoungpeoplewith22q112deletionsyndromeacrosssectionalstudyofslowwavesspindlesandcorrelationswithmemoryandneurodevelopmentalsymptoms AT jessicahhall sleepeeginyoungpeoplewith22q112deletionsyndromeacrosssectionalstudyofslowwavesspindlesandcorrelationswithmemoryandneurodevelopmentalsymptoms AT jeremyhall sleepeeginyoungpeoplewith22q112deletionsyndromeacrosssectionalstudyofslowwavesspindlesandcorrelationswithmemoryandneurodevelopmentalsymptoms AT michaeljowen sleepeeginyoungpeoplewith22q112deletionsyndromeacrosssectionalstudyofslowwavesspindlesandcorrelationswithmemoryandneurodevelopmentalsymptoms AT mariannebmvandenbree sleepeeginyoungpeoplewith22q112deletionsyndromeacrosssectionalstudyofslowwavesspindlesandcorrelationswithmemoryandneurodevelopmentalsymptoms AT mattwjones sleepeeginyoungpeoplewith22q112deletionsyndromeacrosssectionalstudyofslowwavesspindlesandcorrelationswithmemoryandneurodevelopmentalsymptoms |