Detection of extended spectrum β-lactamase in <it>Pseudomonas</it> spp. isolated from two tertiary care hospitals in Bangladesh
<p>Abstract</p> <p>Background</p> <p>Extended spectrum ß-lactamases (ESBLs) represent a major group of lactamases responsible for resistance, mostly produced by gram-negative bacteria, to newer generations of ß-lactam drugs currently being identified in large numbers wo...
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| Format: | Article |
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BMC
2013-01-01
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| Series: | BMC Research Notes |
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| Online Access: | http://www.biomedcentral.com/1756-0500/6/7 |
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| author | Begum Shahanara Salam Md Abdus Alam Kh Faisal Begum Nurjahan Hassan Pervez Haq Jalaluddin Ashraful |
| author_facet | Begum Shahanara Salam Md Abdus Alam Kh Faisal Begum Nurjahan Hassan Pervez Haq Jalaluddin Ashraful |
| author_sort | Begum Shahanara |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Extended spectrum ß-lactamases (ESBLs) represent a major group of lactamases responsible for resistance, mostly produced by gram-negative bacteria, to newer generations of ß-lactam drugs currently being identified in large numbers worldwide. The present study was undertaken to see the frequency of ESBL producing <it>Pseudomonas</it> spp. isolated from six hundred clinical specimens (wound, pus, aural, urine, sputum, throat and other swabs) collected over a period of three years from two tertiary care hospitals in Bangladesh.</p> <p>Findings</p> <p>Aerobic bacterial culture was performed on aseptically collected swabs and only growth of <it>Pseudomonas</it> was considered for further species identification and ESBL production along with serotyping of <it>Pseudomonas aeruginosa</it>. Antimicrobial susceptibility testing was carried out using the Kirby-Bauer agar diffusion method and ESBL production was detected on Mueller Hinton agar by double-disk synergy technique using Amoxicillin-Clavulanic acid with Ceftazidime, Cefotaxime, Ceftriaxone and Aztreonam. Culture yielded 120 <it>Pseudomonas</it> spp<it>.</it> and 82 of them were biochemically characterized for species. <it>Pseudomonas aeruginosa</it> was found to be the predominant (90.2%) species. Of 82 isolates tested for ESBL, 31 (37.8%) were ESBL positive with 29 (93.5%) as <it>Pseudomonas aeruginosa,</it> the remaining 2 (6.5%) were <it>Stenotrophomonas maltophilia</it> and <it>Ralstonia pickettii.</it> Antibiogram revealed Imipenem as the most effective drug (93.3%) among all antimicrobials used against <it>Pseudomonas</it> spp. followed by Aminoglycosides (63.7%).</p> <p>Conclusion</p> <p>ESBL producing <it>Pseudomonas</it> spp. was found to be a frequent isolate from two tertiary care hospitals in Bangladesh, showing limited susceptibility to antimicrobials and decreased susceptibility to Imipenem in particular, which is a matter of great concern.</p> |
| first_indexed | 2024-12-17T08:59:28Z |
| format | Article |
| id | doaj.art-10e1b6cbdfb44eb9b4d93de21acb2113 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-17T08:59:28Z |
| publishDate | 2013-01-01 |
| publisher | BMC |
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| spelling | doaj.art-10e1b6cbdfb44eb9b4d93de21acb21132022-12-21T21:55:49ZengBMCBMC Research Notes1756-05002013-01-0161710.1186/1756-0500-6-7Detection of extended spectrum β-lactamase in <it>Pseudomonas</it> spp. isolated from two tertiary care hospitals in BangladeshBegum ShahanaraSalam Md AbdusAlam Kh FaisalBegum NurjahanHassan PervezHaq Jalaluddin Ashraful<p>Abstract</p> <p>Background</p> <p>Extended spectrum ß-lactamases (ESBLs) represent a major group of lactamases responsible for resistance, mostly produced by gram-negative bacteria, to newer generations of ß-lactam drugs currently being identified in large numbers worldwide. The present study was undertaken to see the frequency of ESBL producing <it>Pseudomonas</it> spp. isolated from six hundred clinical specimens (wound, pus, aural, urine, sputum, throat and other swabs) collected over a period of three years from two tertiary care hospitals in Bangladesh.</p> <p>Findings</p> <p>Aerobic bacterial culture was performed on aseptically collected swabs and only growth of <it>Pseudomonas</it> was considered for further species identification and ESBL production along with serotyping of <it>Pseudomonas aeruginosa</it>. Antimicrobial susceptibility testing was carried out using the Kirby-Bauer agar diffusion method and ESBL production was detected on Mueller Hinton agar by double-disk synergy technique using Amoxicillin-Clavulanic acid with Ceftazidime, Cefotaxime, Ceftriaxone and Aztreonam. Culture yielded 120 <it>Pseudomonas</it> spp<it>.</it> and 82 of them were biochemically characterized for species. <it>Pseudomonas aeruginosa</it> was found to be the predominant (90.2%) species. Of 82 isolates tested for ESBL, 31 (37.8%) were ESBL positive with 29 (93.5%) as <it>Pseudomonas aeruginosa,</it> the remaining 2 (6.5%) were <it>Stenotrophomonas maltophilia</it> and <it>Ralstonia pickettii.</it> Antibiogram revealed Imipenem as the most effective drug (93.3%) among all antimicrobials used against <it>Pseudomonas</it> spp. followed by Aminoglycosides (63.7%).</p> <p>Conclusion</p> <p>ESBL producing <it>Pseudomonas</it> spp. was found to be a frequent isolate from two tertiary care hospitals in Bangladesh, showing limited susceptibility to antimicrobials and decreased susceptibility to Imipenem in particular, which is a matter of great concern.</p>http://www.biomedcentral.com/1756-0500/6/7<it>Pseudomonas</it> sppAntimicrobial susceptibilityESBL |
| spellingShingle | Begum Shahanara Salam Md Abdus Alam Kh Faisal Begum Nurjahan Hassan Pervez Haq Jalaluddin Ashraful Detection of extended spectrum β-lactamase in <it>Pseudomonas</it> spp. isolated from two tertiary care hospitals in Bangladesh BMC Research Notes <it>Pseudomonas</it> spp Antimicrobial susceptibility ESBL |
| title | Detection of extended spectrum β-lactamase in <it>Pseudomonas</it> spp. isolated from two tertiary care hospitals in Bangladesh |
| title_full | Detection of extended spectrum β-lactamase in <it>Pseudomonas</it> spp. isolated from two tertiary care hospitals in Bangladesh |
| title_fullStr | Detection of extended spectrum β-lactamase in <it>Pseudomonas</it> spp. isolated from two tertiary care hospitals in Bangladesh |
| title_full_unstemmed | Detection of extended spectrum β-lactamase in <it>Pseudomonas</it> spp. isolated from two tertiary care hospitals in Bangladesh |
| title_short | Detection of extended spectrum β-lactamase in <it>Pseudomonas</it> spp. isolated from two tertiary care hospitals in Bangladesh |
| title_sort | detection of extended spectrum β lactamase in it pseudomonas it spp isolated from two tertiary care hospitals in bangladesh |
| topic | <it>Pseudomonas</it> spp Antimicrobial susceptibility ESBL |
| url | http://www.biomedcentral.com/1756-0500/6/7 |
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