Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina
Quantitative estimation of vegetative and stress forms of Spirulina platensis and Scenedesmus obliquus as well as vegetative form of Dunaliella salina revealed that S. obliquus constituted highest polysaccharide content than other tested microalgae. The isolated polysaccharides characterized as hete...
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| Format: | Article |
| Language: | English |
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Ain Shams University
2018-12-01
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| Series: | Archives of Pharmaceutical Sciences Ain Shams University |
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| Online Access: | https://aps.journals.ekb.eg/article_28021.html |
| _version_ | 1818666208518471680 |
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| author | Abdel Nasser Singab Nabaweya Ibrahim Abo El-khair Elsayed Waled El-Senousy Hanan Aly Asmaa Abd Elsamiae Azza Abdelmageed Matloub |
| author_facet | Abdel Nasser Singab Nabaweya Ibrahim Abo El-khair Elsayed Waled El-Senousy Hanan Aly Asmaa Abd Elsamiae Azza Abdelmageed Matloub |
| author_sort | Abdel Nasser Singab |
| collection | DOAJ |
| description | Quantitative estimation of vegetative and stress forms of Spirulina platensis and Scenedesmus obliquus as well as vegetative form of Dunaliella salina revealed that S. obliquus constituted highest polysaccharide content than other tested microalgae. The isolated polysaccharides characterized as heterogeneous polysaccharides bounded protein by FT-IR, GLC and Elemental Microanalysis. These polysaccharides constituted of 47-66% of sugar and 14.88-41.06% of protein contents whereas galactose, mannose, glucose and rhamnose were represented as predominant sugar in isolated polysaccharides.
The isolated polysaccharides were evaluated in vitro as antiviral, cytotoxic, antioxidant and anti-cholinesterase properties. The non-toxic dose of isolated polysaccharides on Huh 7.5, MA104, BGM and Vero cell lines were determined. The S. platensis (CEM and HEM) polysaccharides have promising antiviral which reduced replication up to 50–87.6% of HCV genotype 4a replicon, coxsackievirus B4, rotavirus and herpes simplex type 1 virus at non toxic doses 1.8 and 1.5 mg/ml, respectively.
Furthermore, the isolated polysaccharides were assessed for in-vitro cytotoxic activity against MCF-7, HepG2 and HCT116 cell lines. The cytotoxic activity revealed that D. salina HEM polysaccharide show potent cytotoxic activity against HCT 116 cell line with IC50 64.2 μg/ml. Additionally, the isolated polysaccharides showed DPPH• scavenging activity in dose dependent relationship and D. salina HEM and S. obliquus CEM showed the significantly highest activity (308.16 and 308.69%, respectively) at 100 μg/ml. Furthermore, S. obliquus CEM and HEM polysaccharides exhibited the significant highest cholinestearse % inhibition activity.
Microalgal polysaccharides have great therapeutically potential in drug development used as antiviral, antitumour, antioxidant and anticholinestrae agents in near future. |
| first_indexed | 2024-12-17T06:00:53Z |
| format | Article |
| id | doaj.art-10e232ca4bfa4fd697fafa1fb9c79075 |
| institution | Directory Open Access Journal |
| issn | 2356-8380 2356-8399 |
| language | English |
| last_indexed | 2024-12-17T06:00:53Z |
| publishDate | 2018-12-01 |
| publisher | Ain Shams University |
| record_format | Article |
| series | Archives of Pharmaceutical Sciences Ain Shams University |
| spelling | doaj.art-10e232ca4bfa4fd697fafa1fb9c790752022-12-21T22:00:54ZengAin Shams UniversityArchives of Pharmaceutical Sciences Ain Shams University2356-83802356-83992018-12-0122121137https://dx.doi.org/10.21608/aps.2018.18740Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salinaAbdel Nasser Singab0Nabaweya Ibrahim1Abo El-khair Elsayed2Waled El-Senousy3Hanan Aly4Asmaa Abd Elsamiae5Azza Abdelmageed Matloub6Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, Abassia, EgyptPharmacognosy Dept., National Research Centre, Cairo, EgyptAlgal Biotechnology Unit, National Research Centre, 33 Bohouth Street, Dokki, Cairo, EgyptWater Pollution Research Department (Virology Lab), National Research Centre, 33 Bohouth Street, Dokki, Cairo, Egypt.Therapeutic Chemistry Department, National Research Centre, 33 Bohouth Street, Dokki, Cairo, EgyptPharmacognosy Department, National Research Centre, 33 Bohouth Street, Dokki, Cairo, EgyptPharmacognosy Dept., National Research Centre, Cairo, EgyptQuantitative estimation of vegetative and stress forms of Spirulina platensis and Scenedesmus obliquus as well as vegetative form of Dunaliella salina revealed that S. obliquus constituted highest polysaccharide content than other tested microalgae. The isolated polysaccharides characterized as heterogeneous polysaccharides bounded protein by FT-IR, GLC and Elemental Microanalysis. These polysaccharides constituted of 47-66% of sugar and 14.88-41.06% of protein contents whereas galactose, mannose, glucose and rhamnose were represented as predominant sugar in isolated polysaccharides. The isolated polysaccharides were evaluated in vitro as antiviral, cytotoxic, antioxidant and anti-cholinesterase properties. The non-toxic dose of isolated polysaccharides on Huh 7.5, MA104, BGM and Vero cell lines were determined. The S. platensis (CEM and HEM) polysaccharides have promising antiviral which reduced replication up to 50–87.6% of HCV genotype 4a replicon, coxsackievirus B4, rotavirus and herpes simplex type 1 virus at non toxic doses 1.8 and 1.5 mg/ml, respectively. Furthermore, the isolated polysaccharides were assessed for in-vitro cytotoxic activity against MCF-7, HepG2 and HCT116 cell lines. The cytotoxic activity revealed that D. salina HEM polysaccharide show potent cytotoxic activity against HCT 116 cell line with IC50 64.2 μg/ml. Additionally, the isolated polysaccharides showed DPPH• scavenging activity in dose dependent relationship and D. salina HEM and S. obliquus CEM showed the significantly highest activity (308.16 and 308.69%, respectively) at 100 μg/ml. Furthermore, S. obliquus CEM and HEM polysaccharides exhibited the significant highest cholinestearse % inhibition activity. Microalgal polysaccharides have great therapeutically potential in drug development used as antiviral, antitumour, antioxidant and anticholinestrae agents in near future.https://aps.journals.ekb.eg/article_28021.htmlMicroalgaePolysaccharidesantiviral activitycytotoxic activityantioxidant and anti-cholinesterase |
| spellingShingle | Abdel Nasser Singab Nabaweya Ibrahim Abo El-khair Elsayed Waled El-Senousy Hanan Aly Asmaa Abd Elsamiae Azza Abdelmageed Matloub Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina Archives of Pharmaceutical Sciences Ain Shams University Microalgae Polysaccharides antiviral activity cytotoxic activity antioxidant and anti-cholinesterase |
| title | Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina |
| title_full | Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina |
| title_fullStr | Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina |
| title_full_unstemmed | Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina |
| title_short | Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina |
| title_sort | antiviral cytotoxic antioxidant and anti cholinesterase activities of polysaccharides isolated from microalgae spirulina platensis scenedesmus obliquus and dunaliella salina |
| topic | Microalgae Polysaccharides antiviral activity cytotoxic activity antioxidant and anti-cholinesterase |
| url | https://aps.journals.ekb.eg/article_28021.html |
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