Pig Urinary Concentration of Mycotoxins and Metabolites Reflects Regional Differences, Mycotoxin Intake and Feed Contaminations
The determination of mycotoxin and metabolite concentrations in human and animal urine is currently used for risk assessment and mycotoxin intake measurement. In this study, pig urine (<i>n</i> = 195) was collected at slaughterhouses in 2012 by the Swedish National Food Agency in three c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-06-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/7/378 |
| _version_ | 1798025681417797632 |
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| author | Lucia Gambacorta Monica Olsen Michele Solfrizzo |
| author_facet | Lucia Gambacorta Monica Olsen Michele Solfrizzo |
| author_sort | Lucia Gambacorta |
| collection | DOAJ |
| description | The determination of mycotoxin and metabolite concentrations in human and animal urine is currently used for risk assessment and mycotoxin intake measurement. In this study, pig urine (<i>n</i> = 195) was collected at slaughterhouses in 2012 by the Swedish National Food Agency in three counties representing East, South and West regions of Sweden. Urinary concentrations of four mycotoxins, (deoxynivalenol (DON), zearalenone (ZEA), fumonisin B<sub>1</sub> (FB<sub>1</sub>), and ochratoxin A (OTA)), and four key metabolites, (deepoxy-deoxynivalenol (DOM-1), aflatoxin M<sub>1</sub> (AFM<sub>1</sub>, biomarker of AFB<sub>1</sub>), α-zearalenol (α-ZOL), and β-zearalenol (β-ZOL)) were identified and measured by UPLC-MS/MS. Statistically significant regional differences were detected for both total DON (DON + DOM-1) and total ZEA (ZEA + α-ZOL + β-ZOL) concentrations in pig urine from the three regions. These regional differences were in good agreement with the occurrence of <i>Fusarium graminearum</i> mycotoxins (DON + ZEA) in cereal grains harvested in 2011 in Sweden. There were no statistically significant differences in FB<sub>1</sub>, AFM<sub>1</sub> and OTA urinary concentrations in pigs from the three regions. The overall incidence of positive samples was high for total ZEA (99−100%), total DON (96−100%) and OTA (85−95%), medium for FB<sub>1</sub> (30−61%) and low for AFM<sub>1</sub> (0−13%) in the three regions. Urinary mycotoxin biomarker concentrations were used to estimate mycotoxin intake and the level of mycotoxins in feeds consumed by the monitored pigs. The back-calculated levels of mycotoxins in feeds were low with the exception of seven samples that were higher the European limits. |
| first_indexed | 2024-04-11T18:23:52Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T18:23:52Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-10e78639cf5349c8988be73fac4e23f12022-12-22T04:09:42ZengMDPI AGToxins2072-66512019-06-0111737810.3390/toxins11070378toxins11070378Pig Urinary Concentration of Mycotoxins and Metabolites Reflects Regional Differences, Mycotoxin Intake and Feed ContaminationsLucia Gambacorta0Monica Olsen1Michele Solfrizzo2Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via Amendola 122/O, 70126 Bari, ItalyNational Food Agency, Department of Risk Benefit Assessment, P.O. Box 622, 751 26 Uppsala, SwedenInstitute of Sciences of Food Production (ISPA), National Research Council (CNR), Via Amendola 122/O, 70126 Bari, ItalyThe determination of mycotoxin and metabolite concentrations in human and animal urine is currently used for risk assessment and mycotoxin intake measurement. In this study, pig urine (<i>n</i> = 195) was collected at slaughterhouses in 2012 by the Swedish National Food Agency in three counties representing East, South and West regions of Sweden. Urinary concentrations of four mycotoxins, (deoxynivalenol (DON), zearalenone (ZEA), fumonisin B<sub>1</sub> (FB<sub>1</sub>), and ochratoxin A (OTA)), and four key metabolites, (deepoxy-deoxynivalenol (DOM-1), aflatoxin M<sub>1</sub> (AFM<sub>1</sub>, biomarker of AFB<sub>1</sub>), α-zearalenol (α-ZOL), and β-zearalenol (β-ZOL)) were identified and measured by UPLC-MS/MS. Statistically significant regional differences were detected for both total DON (DON + DOM-1) and total ZEA (ZEA + α-ZOL + β-ZOL) concentrations in pig urine from the three regions. These regional differences were in good agreement with the occurrence of <i>Fusarium graminearum</i> mycotoxins (DON + ZEA) in cereal grains harvested in 2011 in Sweden. There were no statistically significant differences in FB<sub>1</sub>, AFM<sub>1</sub> and OTA urinary concentrations in pigs from the three regions. The overall incidence of positive samples was high for total ZEA (99−100%), total DON (96−100%) and OTA (85−95%), medium for FB<sub>1</sub> (30−61%) and low for AFM<sub>1</sub> (0−13%) in the three regions. Urinary mycotoxin biomarker concentrations were used to estimate mycotoxin intake and the level of mycotoxins in feeds consumed by the monitored pigs. The back-calculated levels of mycotoxins in feeds were low with the exception of seven samples that were higher the European limits.https://www.mdpi.com/2072-6651/11/7/378mycotoxinsbiomarkersurineUPLC-MS/MSintakefeedgrain |
| spellingShingle | Lucia Gambacorta Monica Olsen Michele Solfrizzo Pig Urinary Concentration of Mycotoxins and Metabolites Reflects Regional Differences, Mycotoxin Intake and Feed Contaminations Toxins mycotoxins biomarkers urine UPLC-MS/MS intake feed grain |
| title | Pig Urinary Concentration of Mycotoxins and Metabolites Reflects Regional Differences, Mycotoxin Intake and Feed Contaminations |
| title_full | Pig Urinary Concentration of Mycotoxins and Metabolites Reflects Regional Differences, Mycotoxin Intake and Feed Contaminations |
| title_fullStr | Pig Urinary Concentration of Mycotoxins and Metabolites Reflects Regional Differences, Mycotoxin Intake and Feed Contaminations |
| title_full_unstemmed | Pig Urinary Concentration of Mycotoxins and Metabolites Reflects Regional Differences, Mycotoxin Intake and Feed Contaminations |
| title_short | Pig Urinary Concentration of Mycotoxins and Metabolites Reflects Regional Differences, Mycotoxin Intake and Feed Contaminations |
| title_sort | pig urinary concentration of mycotoxins and metabolites reflects regional differences mycotoxin intake and feed contaminations |
| topic | mycotoxins biomarkers urine UPLC-MS/MS intake feed grain |
| url | https://www.mdpi.com/2072-6651/11/7/378 |
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