Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1
Abstract Poly(thionine)-Au, a novel multifunctional substrate with excellent redox signal, enzyme-like activity, and easy antibody immobilisation, was synthesised using HAuCl4 as the oxidising agent and thionine as the monomer. The prepared poly(thionine)-Au composite exhibited an admirable electroc...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-01250-0 |
| _version_ | 1818343822313127936 |
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| author | Huiqiang Wang Xin Gao Zhanfang Ma |
| author_facet | Huiqiang Wang Xin Gao Zhanfang Ma |
| author_sort | Huiqiang Wang |
| collection | DOAJ |
| description | Abstract Poly(thionine)-Au, a novel multifunctional substrate with excellent redox signal, enzyme-like activity, and easy antibody immobilisation, was synthesised using HAuCl4 as the oxidising agent and thionine as the monomer. The prepared poly(thionine)-Au composite exhibited an admirable electrochemical redox signal at −0.15 V and excellent H2O2 catalytic ability. In addition, gold nanoparticles in this composite were found to directly immobilise antibodies and further improve conductivity. In addition, a label-free electrochemical immunosensor was developed using poly(thionine)-Au as the sensing substrate for ultrasensitive detection of cytokeratin antigen 21-1 (CYFRA 21-1), an immunoassay found in human serum. The prepared immunosensor showed a wide liner range from 100 ng mL−1 to 10 fg mL−1 and an ultralow detection limit of 4.6 fg mL−1 (the ratio of signal to noise (S/N) = 3). Additionally, this method was used to analyse human serum samples and yielded results consistency with those of ELISA, implying its potential application in clinical research. The poly(thionine)-Au composite can be easily extended to other polymer-based nanocomposites, which is significant for other electrochemical immunoassays. |
| first_indexed | 2024-12-13T16:36:41Z |
| format | Article |
| id | doaj.art-10e7bfef4e0f4379a9e588095cfd6848 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T16:36:41Z |
| publishDate | 2017-04-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-10e7bfef4e0f4379a9e588095cfd68482022-12-21T23:38:22ZengNature PortfolioScientific Reports2045-23222017-04-01711710.1038/s41598-017-01250-0Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1Huiqiang Wang0Xin Gao1Zhanfang Ma2Department of Chemistry, Capital Normal UniversityDepartment of Chemistry, Capital Normal UniversityDepartment of Chemistry, Capital Normal UniversityAbstract Poly(thionine)-Au, a novel multifunctional substrate with excellent redox signal, enzyme-like activity, and easy antibody immobilisation, was synthesised using HAuCl4 as the oxidising agent and thionine as the monomer. The prepared poly(thionine)-Au composite exhibited an admirable electrochemical redox signal at −0.15 V and excellent H2O2 catalytic ability. In addition, gold nanoparticles in this composite were found to directly immobilise antibodies and further improve conductivity. In addition, a label-free electrochemical immunosensor was developed using poly(thionine)-Au as the sensing substrate for ultrasensitive detection of cytokeratin antigen 21-1 (CYFRA 21-1), an immunoassay found in human serum. The prepared immunosensor showed a wide liner range from 100 ng mL−1 to 10 fg mL−1 and an ultralow detection limit of 4.6 fg mL−1 (the ratio of signal to noise (S/N) = 3). Additionally, this method was used to analyse human serum samples and yielded results consistency with those of ELISA, implying its potential application in clinical research. The poly(thionine)-Au composite can be easily extended to other polymer-based nanocomposites, which is significant for other electrochemical immunoassays.https://doi.org/10.1038/s41598-017-01250-0 |
| spellingShingle | Huiqiang Wang Xin Gao Zhanfang Ma Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1 Scientific Reports |
| title | Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1 |
| title_full | Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1 |
| title_fullStr | Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1 |
| title_full_unstemmed | Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1 |
| title_short | Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1 |
| title_sort | multifunctional substrate of label free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21 1 |
| url | https://doi.org/10.1038/s41598-017-01250-0 |
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