Bile acid formation in man: metabolism of 7α-hydroxy-4-cholesten-3-one in bile fistula patients
7α-Hydroxy-4-cholesten-3-one is thought to be an intermediate in human bile acid synthesis. This conclusion is based on in vivo experiments in animals and on in vitro studies in which homogenates of animal and human livers were used. To further establish that this compound is an intermediate in huma...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1973-01-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520393287 |
| _version_ | 1831737703587119104 |
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| author | Russell F. Hanson Peter D. Klein Gale C. Williams |
| author_facet | Russell F. Hanson Peter D. Klein Gale C. Williams |
| author_sort | Russell F. Hanson |
| collection | DOAJ |
| description | 7α-Hydroxy-4-cholesten-3-one is thought to be an intermediate in human bile acid synthesis. This conclusion is based on in vivo experiments in animals and on in vitro studies in which homogenates of animal and human livers were used. To further establish that this compound is an intermediate in human bile acid synthesis, its metabolism was studied in subjects with complete bile fistulas. After administration of 3H-labeled 7α-hydroxy-4-cholesten-3-one by single intravenous injection, approximately 85% of the administered isotope was recovered in the bile during the first 12 hr. More than 96% of the radioactivity recovered in the bile was identified as either chenodeoxycholic acid or cholic acid, with only a trace amount of the radioactivity present as neutral sterols. This study gives support to the hypothesis that 7α-hydroxy-4-cholesten-3-one is a natural intermediate in human bile acid synthesis. |
| first_indexed | 2024-12-21T13:08:34Z |
| format | Article |
| id | doaj.art-10e837bad99f4356bd79aad80133a254 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T13:08:34Z |
| publishDate | 1973-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-10e837bad99f4356bd79aad80133a2542022-12-21T19:02:57ZengElsevierJournal of Lipid Research0022-22751973-01-011415053Bile acid formation in man: metabolism of 7α-hydroxy-4-cholesten-3-one in bile fistula patientsRussell F. Hanson0Peter D. Klein1Gale C. Williams2Gastroenterology Unit, Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455, and; Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439Gastroenterology Unit, Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455, and; Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439Gastroenterology Unit, Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455, and; Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 604397α-Hydroxy-4-cholesten-3-one is thought to be an intermediate in human bile acid synthesis. This conclusion is based on in vivo experiments in animals and on in vitro studies in which homogenates of animal and human livers were used. To further establish that this compound is an intermediate in human bile acid synthesis, its metabolism was studied in subjects with complete bile fistulas. After administration of 3H-labeled 7α-hydroxy-4-cholesten-3-one by single intravenous injection, approximately 85% of the administered isotope was recovered in the bile during the first 12 hr. More than 96% of the radioactivity recovered in the bile was identified as either chenodeoxycholic acid or cholic acid, with only a trace amount of the radioactivity present as neutral sterols. This study gives support to the hypothesis that 7α-hydroxy-4-cholesten-3-one is a natural intermediate in human bile acid synthesis.http://www.sciencedirect.com/science/article/pii/S0022227520393287cholic acidchenodeoxycholic acid |
| spellingShingle | Russell F. Hanson Peter D. Klein Gale C. Williams Bile acid formation in man: metabolism of 7α-hydroxy-4-cholesten-3-one in bile fistula patients Journal of Lipid Research cholic acid chenodeoxycholic acid |
| title | Bile acid formation in man: metabolism of 7α-hydroxy-4-cholesten-3-one in bile fistula patients |
| title_full | Bile acid formation in man: metabolism of 7α-hydroxy-4-cholesten-3-one in bile fistula patients |
| title_fullStr | Bile acid formation in man: metabolism of 7α-hydroxy-4-cholesten-3-one in bile fistula patients |
| title_full_unstemmed | Bile acid formation in man: metabolism of 7α-hydroxy-4-cholesten-3-one in bile fistula patients |
| title_short | Bile acid formation in man: metabolism of 7α-hydroxy-4-cholesten-3-one in bile fistula patients |
| title_sort | bile acid formation in man metabolism of 7α hydroxy 4 cholesten 3 one in bile fistula patients |
| topic | cholic acid chenodeoxycholic acid |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520393287 |
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