Sodium selenite promotes osteoblast differentiation via the WNT/β-catenin signaling pathway
Objective: Osteoporosis is regarded as a silent disorder affecting bone slowly, leading to an increased risk of fractures. Lately, selenium has been found to be associated with the acquisition and maintenance of bone health by affecting the bone remodeling process. However, the mechanism of action o...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2022-06-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | https://www.celljournal.org/article_253282_ee6edbfb94e3d9e79d11150088bde0a9.pdf |
| _version_ | 1828189544143388672 |
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| author | Ashish Ranjan Sharma Garima Sharma Yeon-Hee Lee Chiranjib Chakraborty Sang-Soo Lee Eun-Min Seo |
| author_facet | Ashish Ranjan Sharma Garima Sharma Yeon-Hee Lee Chiranjib Chakraborty Sang-Soo Lee Eun-Min Seo |
| author_sort | Ashish Ranjan Sharma |
| collection | DOAJ |
| description | Objective: Osteoporosis is regarded as a silent disorder affecting bone slowly, leading to an increased risk of fractures. Lately, selenium has been found to be associated with the acquisition and maintenance of bone health by affecting the bone remodeling process. However, the mechanism of action of selenium on bone is poorly understood. Here, the protective effects of sodium selenite on the differentiation process of osteoblasts as well as under oxidative stress-induced conditions were evaluated.Materials and Methods: MC3T3-E1 cells, were treated with a various doses (0, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 ug/ml) of sodium selenite. Cell viability and cytotoxicity were observed by MTT and LDH assay. The osteogenic activity and expression level of osteogenic markers were confirmed through ALP activity, real-time RT-PCR and sirius red staining. Role of sodium selenite and involvement of WNT signaling was assessed by Axin-2 reporter assay and western blotting.Results: It was observed that the sodium selenite could promote the ALP activity and collagen synthesis in pre-osteoblasts. Moreover, sodium selenite increased the mRNA expression levels of osteogenic transcriptional factors, such as runt-related transcription factor 2 (Runx2) and osterix (OSX). In addition, the terminal differentiation markers, such as osteocalcin (OCN), and collagen 1α (Col1α), were also increased. Treatment of sodium selenite recused the H2O2-induced inhibition of osteoblastic differentiation of pre-osteoblasts cells. Furthermore, sodium selenite restored the H2O2 repressed β-catenin stability and axin-2 reporter activity in MC3T3-E1 cells implicating involvement of WNT signaling pathway.Conclusion: It may be concluded that selenite can stimulate bone formation and rescue the oxidative repression of osteogenesis by activating WNT signaling pathway and may act as a potential therapeutic intervention for osteoporosis. |
| first_indexed | 2024-04-12T08:07:28Z |
| format | Article |
| id | doaj.art-10e8dc315cc84ff5b9d2891ebf3f48b3 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-12T08:07:28Z |
| publishDate | 2022-06-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-10e8dc315cc84ff5b9d2891ebf3f48b32022-12-22T03:41:05ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142022-06-0124630931510.22074/cellj.2022.8314253282Sodium selenite promotes osteoblast differentiation via the WNT/β-catenin signaling pathwayAshish Ranjan Sharma0Garima Sharma1Yeon-Hee Lee2Chiranjib Chakraborty3Sang-Soo Lee4Eun-Min Seo5Institute for Skeletal Aging & Orthopedic Surgery, Hallym University-Chuncheon Sacred Heart Hospital, Chuncheon-si, 24252, Gangwon-do, Republic of KoreaInstitute for Skeletal Aging & Orthopedic Surgery, Hallym University-Chuncheon Sacred Heart Hospital, Chuncheon-si, 24252, Gangwon-do, Republic of KoreaInstitute for Skeletal Aging & Orthopedic Surgery, Hallym University-Chuncheon Sacred Heart Hospital, Chuncheon-si, 24252, Gangwon-do, Republic of KoreaInstitute for Skeletal Aging and Orthopedic Surgery, Hallym University-Chuncheon Sacred Heart Hospital, Chuncheon, Korea;Department of Bio-Informatics, School of Computer and Information Sciences, Galgotias University,Institute for Skeletal Aging and Orthopedic Surgery, Hallym University-Chuncheon Sacred Heart Hospital, Chuncheon-si, Gangwon-do, Republic of KoreaInstitute for Skeletal Aging & Orthopedic Surgery, Hallym University-Chuncheon Sacred Heart Hospital, Chuncheon-si, 24252, Gangwon-do, Republic of KoreaObjective: Osteoporosis is regarded as a silent disorder affecting bone slowly, leading to an increased risk of fractures. Lately, selenium has been found to be associated with the acquisition and maintenance of bone health by affecting the bone remodeling process. However, the mechanism of action of selenium on bone is poorly understood. Here, the protective effects of sodium selenite on the differentiation process of osteoblasts as well as under oxidative stress-induced conditions were evaluated.Materials and Methods: MC3T3-E1 cells, were treated with a various doses (0, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 ug/ml) of sodium selenite. Cell viability and cytotoxicity were observed by MTT and LDH assay. The osteogenic activity and expression level of osteogenic markers were confirmed through ALP activity, real-time RT-PCR and sirius red staining. Role of sodium selenite and involvement of WNT signaling was assessed by Axin-2 reporter assay and western blotting.Results: It was observed that the sodium selenite could promote the ALP activity and collagen synthesis in pre-osteoblasts. Moreover, sodium selenite increased the mRNA expression levels of osteogenic transcriptional factors, such as runt-related transcription factor 2 (Runx2) and osterix (OSX). In addition, the terminal differentiation markers, such as osteocalcin (OCN), and collagen 1α (Col1α), were also increased. Treatment of sodium selenite recused the H2O2-induced inhibition of osteoblastic differentiation of pre-osteoblasts cells. Furthermore, sodium selenite restored the H2O2 repressed β-catenin stability and axin-2 reporter activity in MC3T3-E1 cells implicating involvement of WNT signaling pathway.Conclusion: It may be concluded that selenite can stimulate bone formation and rescue the oxidative repression of osteogenesis by activating WNT signaling pathway and may act as a potential therapeutic intervention for osteoporosis.https://www.celljournal.org/article_253282_ee6edbfb94e3d9e79d11150088bde0a9.pdfosteoblastsosteoporosisseleniumwnt signaling pathway |
| spellingShingle | Ashish Ranjan Sharma Garima Sharma Yeon-Hee Lee Chiranjib Chakraborty Sang-Soo Lee Eun-Min Seo Sodium selenite promotes osteoblast differentiation via the WNT/β-catenin signaling pathway Cell Journal osteoblasts osteoporosis selenium wnt signaling pathway |
| title | Sodium selenite promotes osteoblast differentiation via the WNT/β-catenin signaling pathway |
| title_full | Sodium selenite promotes osteoblast differentiation via the WNT/β-catenin signaling pathway |
| title_fullStr | Sodium selenite promotes osteoblast differentiation via the WNT/β-catenin signaling pathway |
| title_full_unstemmed | Sodium selenite promotes osteoblast differentiation via the WNT/β-catenin signaling pathway |
| title_short | Sodium selenite promotes osteoblast differentiation via the WNT/β-catenin signaling pathway |
| title_sort | sodium selenite promotes osteoblast differentiation via the wnt β catenin signaling pathway |
| topic | osteoblasts osteoporosis selenium wnt signaling pathway |
| url | https://www.celljournal.org/article_253282_ee6edbfb94e3d9e79d11150088bde0a9.pdf |
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