The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs
Background: Visceral leishmaniasis (VL) is the most severe form of leishmaniasis in Iran with high mortality rates in the case of inaccurate diagnosis and treatment. This study aimed to prepare and evaluate a new rk39 recombinant antigen from an Iranian strain of Leishmania infantum for diagnosis of...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-03-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2834 |
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| author | Bibi Razieh HOSSEINI FARASH Mehdi MOHEBALI Bahram KAZEMI Homa HAJJARAN Abdolmajid FATA Reza RAOOFIAN Behnaz AKHOUNDI Majid MOJARRAD Pietro MASTROENI Mohammad Kazem SHARIFI-YAZDI Mohammad Hossein TANIPOUR |
| author_facet | Bibi Razieh HOSSEINI FARASH Mehdi MOHEBALI Bahram KAZEMI Homa HAJJARAN Abdolmajid FATA Reza RAOOFIAN Behnaz AKHOUNDI Majid MOJARRAD Pietro MASTROENI Mohammad Kazem SHARIFI-YAZDI Mohammad Hossein TANIPOUR |
| author_sort | Bibi Razieh HOSSEINI FARASH |
| collection | DOAJ |
| description | Background: Visceral leishmaniasis (VL) is the most severe form of leishmaniasis in Iran with high mortality rates in the case of inaccurate diagnosis and treatment. This study aimed to prepare and evaluate a new rk39 recombinant antigen from an Iranian strain of Leishmania infantum for diagnosis of VL in humans and dogs.
Methods: rK39-based enzyme-linked immunosorbent assay (ELISA) was compared with the direct agglutination test (DAT) for the detection of anti L. infantum antibodies. We screened 84 human sera and 87 dog sera from clinical cases in the endemic area of Meshkin-Shahr, Iran along with 176 sera from healthy controls (collected from 86 humans and 90 dogs) during 2013 -2016.
Results: Using the rK39 ELISA, a sensitivity of 85.7% (95% CI, 95-99%) and a specificity of 86.0% (95% CI, 95%-99%) were detected in human sera at a 1:800 (cut-off) titer when DAT-confirmed cases were compared with healthy controls; a sensitivity of 96.6% (95% CI, 95%-99%) and specificity of 94.4% (95% CI, 95%-99%) were found at a 1:80 (cut-off) titer compared with DAT. Kappa analysis indicated agreement between the rK39 ELISA and DAT (0.718) when using human sera at a 1:800 (cut-off) titer as well as (0.910) at a 1:80 (cut-off) titer when using dog sera (P<0.05).
Conclusion: New rk39 recombinant antigen from an Iranian strain of Leishmania infantum seems to be used for diagnosis of VL in humans and dogs. Further extended field studies are recommended. |
| first_indexed | 2024-12-19T15:52:48Z |
| format | Article |
| id | doaj.art-10ea88108677424ab7745b8df109502b |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-19T15:52:48Z |
| publishDate | 2020-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-10ea88108677424ab7745b8df109502b2022-12-21T20:15:10ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2020-03-0115110.18502/ijpa.v15i1.25262834The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and DogsBibi Razieh HOSSEINI FARASH0Mehdi MOHEBALI1Bahram KAZEMI2Homa HAJJARAN3Abdolmajid FATA4Reza RAOOFIAN5Behnaz AKHOUNDI6Majid MOJARRAD7Pietro MASTROENI8Mohammad Kazem SHARIFI-YAZDI9Mohammad Hossein TANIPOUR10Department of Medical Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AND Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, IranDepartment of Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AND Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, IranLegal Medicine Research Center, Legal Medicine Organization, Tehran, IranDepartment of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Veterinary Medicine, University of Cambridge, Cambridge, United KingdomCenter for Research of Zoonoses of Iran, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranBackground: Visceral leishmaniasis (VL) is the most severe form of leishmaniasis in Iran with high mortality rates in the case of inaccurate diagnosis and treatment. This study aimed to prepare and evaluate a new rk39 recombinant antigen from an Iranian strain of Leishmania infantum for diagnosis of VL in humans and dogs. Methods: rK39-based enzyme-linked immunosorbent assay (ELISA) was compared with the direct agglutination test (DAT) for the detection of anti L. infantum antibodies. We screened 84 human sera and 87 dog sera from clinical cases in the endemic area of Meshkin-Shahr, Iran along with 176 sera from healthy controls (collected from 86 humans and 90 dogs) during 2013 -2016. Results: Using the rK39 ELISA, a sensitivity of 85.7% (95% CI, 95-99%) and a specificity of 86.0% (95% CI, 95%-99%) were detected in human sera at a 1:800 (cut-off) titer when DAT-confirmed cases were compared with healthy controls; a sensitivity of 96.6% (95% CI, 95%-99%) and specificity of 94.4% (95% CI, 95%-99%) were found at a 1:80 (cut-off) titer compared with DAT. Kappa analysis indicated agreement between the rK39 ELISA and DAT (0.718) when using human sera at a 1:800 (cut-off) titer as well as (0.910) at a 1:80 (cut-off) titer when using dog sera (P<0.05). Conclusion: New rk39 recombinant antigen from an Iranian strain of Leishmania infantum seems to be used for diagnosis of VL in humans and dogs. Further extended field studies are recommended.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2834rK39 recombinant anti-genLeishmania infantumVisceral leishmaniasisHumanDog |
| spellingShingle | Bibi Razieh HOSSEINI FARASH Mehdi MOHEBALI Bahram KAZEMI Homa HAJJARAN Abdolmajid FATA Reza RAOOFIAN Behnaz AKHOUNDI Majid MOJARRAD Pietro MASTROENI Mohammad Kazem SHARIFI-YAZDI Mohammad Hossein TANIPOUR The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs Iranian Journal of Parasitology rK39 recombinant anti-gen Leishmania infantum Visceral leishmaniasis Human Dog |
| title | The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs |
| title_full | The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs |
| title_fullStr | The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs |
| title_full_unstemmed | The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs |
| title_short | The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs |
| title_sort | rk39 antigen from an iranian strain of leishmania infantum detection of anti leishmania antibodies in humans and dogs |
| topic | rK39 recombinant anti-gen Leishmania infantum Visceral leishmaniasis Human Dog |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2834 |
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