Identification of Polyphenol Glucuronide Conjugates in <i>Glechoma hederacea</i> var. <i>longituba</i> Hot Water Extracts by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS)
<i>Glechoma hederacea</i> var. <i>longituba</i> (GHL) is one of many herbal plants distributed worldwide and is known to contain various biologically useful antioxidant constituents. GHL has been used in folk remedies for various treatments and as favorable tea beverages. How...
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MDPI AG
2020-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/20/4713 |
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| author | Kun Cho Yoon-Ji Choi Yeong Hee Ahn |
| author_facet | Kun Cho Yoon-Ji Choi Yeong Hee Ahn |
| author_sort | Kun Cho |
| collection | DOAJ |
| description | <i>Glechoma hederacea</i> var. <i>longituba</i> (GHL) is one of many herbal plants distributed worldwide and is known to contain various biologically useful antioxidant constituents. GHL has been used in folk remedies for various treatments and as favorable tea beverages. However, research on the precise analysis of ingredients in GHL extracts remains insufficient. In this study, compositional analysis has been conducted on polyphenolic ingredients in GHL hot water extracts. GHL samples collected from growing regions were incubated in hot water at 100 °C for 1 h. The polyphenolic constituents in the hot water extracts were analyzed using high performance liquid chromatography-high resolution mass spectrometry (HPLC-HR MS) and tandem mass spectrometry (HPLC-MS/MS) in negative ion mode. As a result, a total of seven compounds were identified as the major polyphenolic constituents. Interestingly, four constituents out of the identified substances were confirmed to be polyphenol glucuronide conjugates, in which glucuronidation was known to be an important metabolic process in polyphenol aglycone along with methylation and sulphation. This study can be applied for the quality control and standardization of GHL herbal samples and the monitoring of metabolic processes involved in the polyphenolic conjugates. |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T15:37:05Z |
| publishDate | 2020-10-01 |
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| spelling | doaj.art-10ebd91dea6a49949658de6b47cf16332023-11-20T17:05:47ZengMDPI AGMolecules1420-30492020-10-012520471310.3390/molecules25204713Identification of Polyphenol Glucuronide Conjugates in <i>Glechoma hederacea</i> var. <i>longituba</i> Hot Water Extracts by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS)Kun Cho0Yoon-Ji Choi1Yeong Hee Ahn2Center for research equipment, Korea basic science institute, Cheongju 28119, KoreaGraduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134, KoreaDepartment of Biomedical Science, Cheongju University, Cheongju 28160, Korea<i>Glechoma hederacea</i> var. <i>longituba</i> (GHL) is one of many herbal plants distributed worldwide and is known to contain various biologically useful antioxidant constituents. GHL has been used in folk remedies for various treatments and as favorable tea beverages. However, research on the precise analysis of ingredients in GHL extracts remains insufficient. In this study, compositional analysis has been conducted on polyphenolic ingredients in GHL hot water extracts. GHL samples collected from growing regions were incubated in hot water at 100 °C for 1 h. The polyphenolic constituents in the hot water extracts were analyzed using high performance liquid chromatography-high resolution mass spectrometry (HPLC-HR MS) and tandem mass spectrometry (HPLC-MS/MS) in negative ion mode. As a result, a total of seven compounds were identified as the major polyphenolic constituents. Interestingly, four constituents out of the identified substances were confirmed to be polyphenol glucuronide conjugates, in which glucuronidation was known to be an important metabolic process in polyphenol aglycone along with methylation and sulphation. This study can be applied for the quality control and standardization of GHL herbal samples and the monitoring of metabolic processes involved in the polyphenolic conjugates.https://www.mdpi.com/1420-3049/25/20/4713<i>Glechoma hederacea</i>polyphenol glucuronide conjugatetandem mass spectrometry |
| spellingShingle | Kun Cho Yoon-Ji Choi Yeong Hee Ahn Identification of Polyphenol Glucuronide Conjugates in <i>Glechoma hederacea</i> var. <i>longituba</i> Hot Water Extracts by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) Molecules <i>Glechoma hederacea</i> polyphenol glucuronide conjugate tandem mass spectrometry |
| title | Identification of Polyphenol Glucuronide Conjugates in <i>Glechoma hederacea</i> var. <i>longituba</i> Hot Water Extracts by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) |
| title_full | Identification of Polyphenol Glucuronide Conjugates in <i>Glechoma hederacea</i> var. <i>longituba</i> Hot Water Extracts by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) |
| title_fullStr | Identification of Polyphenol Glucuronide Conjugates in <i>Glechoma hederacea</i> var. <i>longituba</i> Hot Water Extracts by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) |
| title_full_unstemmed | Identification of Polyphenol Glucuronide Conjugates in <i>Glechoma hederacea</i> var. <i>longituba</i> Hot Water Extracts by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) |
| title_short | Identification of Polyphenol Glucuronide Conjugates in <i>Glechoma hederacea</i> var. <i>longituba</i> Hot Water Extracts by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) |
| title_sort | identification of polyphenol glucuronide conjugates in i glechoma hederacea i var i longituba i hot water extracts by high performance liquid chromatography tandem mass spectrometry hplc ms ms |
| topic | <i>Glechoma hederacea</i> polyphenol glucuronide conjugate tandem mass spectrometry |
| url | https://www.mdpi.com/1420-3049/25/20/4713 |
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