Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges

Demosponges are a well-known source of a plethora of bioactive compounds. In particular, they are able to form a skeleton by direct deposition of silica in a process catalyzed by silicatein. Herein, we isolated biosilicas from five different Atlantic deep-sea sponges Geodia atlantica (GA), Geodia ba...

Full description

Bibliographic Details
Main Authors: Olesia Dudik, Sara Amorim, Joana R. Xavier, Hans Tore Rapp, Tiago H. Silva, Ricardo A. Pires, Rui L. Reis
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-05-01
Series:Frontiers in Marine Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmars.2021.637810/full
_version_ 1819067529566355456
author Olesia Dudik
Olesia Dudik
Sara Amorim
Sara Amorim
Joana R. Xavier
Joana R. Xavier
Hans Tore Rapp
Tiago H. Silva
Tiago H. Silva
Ricardo A. Pires
Ricardo A. Pires
Rui L. Reis
Rui L. Reis
author_facet Olesia Dudik
Olesia Dudik
Sara Amorim
Sara Amorim
Joana R. Xavier
Joana R. Xavier
Hans Tore Rapp
Tiago H. Silva
Tiago H. Silva
Ricardo A. Pires
Ricardo A. Pires
Rui L. Reis
Rui L. Reis
author_sort Olesia Dudik
collection DOAJ
description Demosponges are a well-known source of a plethora of bioactive compounds. In particular, they are able to form a skeleton by direct deposition of silica in a process catalyzed by silicatein. Herein, we isolated biosilicas from five different Atlantic deep-sea sponges Geodia atlantica (GA), Geodia barretti (GB), Stelletta normani (SN), Axinella infundibuliformis (AI), and Phakellia ventilabrum (PV) to explore the bioactivity and osteogenic capacity of its silica-based materials. We chemically characterized the isolated biosilicas and evaluated them for their bioactivity to deposit Ca and P on their surface (by immersion in simulated body fluid, SBF). GB-, SN-, AI-, and PV-based biosilicas did not generate a stable calcium phosphate (CaP) layer over time in the presence of SBF, however, the GA-derived one was able to form a CaP surface layer (at a Ca/P ratio of ∼1.7, similar to the one observed for hydroxyapatite), that was stable during the 28 days of testing. In addition, no cytotoxicity toward L929 and SaOs2 cells was observed for the GA-based biosilica up to a concentration of 10 mg/mL. Overall, the GA-based biosilica presents the characteristics to be used in the development of biomaterials for bone tissue engineering (BTE).
first_indexed 2024-12-21T16:19:42Z
format Article
id doaj.art-10ebead807ec40dd850d26bcc552fa23
institution Directory Open Access Journal
issn 2296-7745
language English
last_indexed 2024-12-21T16:19:42Z
publishDate 2021-05-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Marine Science
spelling doaj.art-10ebead807ec40dd850d26bcc552fa232022-12-21T18:57:36ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-05-01810.3389/fmars.2021.637810637810Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea SpongesOlesia Dudik0Olesia Dudik1Sara Amorim2Sara Amorim3Joana R. Xavier4Joana R. Xavier5Hans Tore Rapp6Tiago H. Silva7Tiago H. Silva8Ricardo A. Pires9Ricardo A. Pires10Rui L. Reis11Rui L. Reis123B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Guimarães, Portugal3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Guimarães, PortugalCIIMAR – Interdisciplinary Centre of Marine and Environmental Research, Terminal de Cruzeiros de Leixões, Matosinhos, PortugalDepartment of Biology and K.G. Jebsen Centre for Deep-sea Research, University of Bergen, Bergen, NorwayDepartment of Biology and K.G. Jebsen Centre for Deep-sea Research, University of Bergen, Bergen, Norway3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Guimarães, Portugal3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Guimarães, Portugal3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Guimarães, PortugalDemosponges are a well-known source of a plethora of bioactive compounds. In particular, they are able to form a skeleton by direct deposition of silica in a process catalyzed by silicatein. Herein, we isolated biosilicas from five different Atlantic deep-sea sponges Geodia atlantica (GA), Geodia barretti (GB), Stelletta normani (SN), Axinella infundibuliformis (AI), and Phakellia ventilabrum (PV) to explore the bioactivity and osteogenic capacity of its silica-based materials. We chemically characterized the isolated biosilicas and evaluated them for their bioactivity to deposit Ca and P on their surface (by immersion in simulated body fluid, SBF). GB-, SN-, AI-, and PV-based biosilicas did not generate a stable calcium phosphate (CaP) layer over time in the presence of SBF, however, the GA-derived one was able to form a CaP surface layer (at a Ca/P ratio of ∼1.7, similar to the one observed for hydroxyapatite), that was stable during the 28 days of testing. In addition, no cytotoxicity toward L929 and SaOs2 cells was observed for the GA-based biosilica up to a concentration of 10 mg/mL. Overall, the GA-based biosilica presents the characteristics to be used in the development of biomaterials for bone tissue engineering (BTE).https://www.frontiersin.org/articles/10.3389/fmars.2021.637810/fullbioactivitybiosilicabioceramicstissue engineeringdeep-sea sponges
spellingShingle Olesia Dudik
Olesia Dudik
Sara Amorim
Sara Amorim
Joana R. Xavier
Joana R. Xavier
Hans Tore Rapp
Tiago H. Silva
Tiago H. Silva
Ricardo A. Pires
Ricardo A. Pires
Rui L. Reis
Rui L. Reis
Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges
Frontiers in Marine Science
bioactivity
biosilica
bioceramics
tissue engineering
deep-sea sponges
title Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges
title_full Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges
title_fullStr Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges
title_full_unstemmed Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges
title_short Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges
title_sort bioactivity of biosilica obtained from north atlantic deep sea sponges
topic bioactivity
biosilica
bioceramics
tissue engineering
deep-sea sponges
url https://www.frontiersin.org/articles/10.3389/fmars.2021.637810/full
work_keys_str_mv AT olesiadudik bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT olesiadudik bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT saraamorim bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT saraamorim bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT joanarxavier bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT joanarxavier bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT hanstorerapp bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT tiagohsilva bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT tiagohsilva bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT ricardoapires bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT ricardoapires bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT ruilreis bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges
AT ruilreis bioactivityofbiosilicaobtainedfromnorthatlanticdeepseasponges