Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges
Demosponges are a well-known source of a plethora of bioactive compounds. In particular, they are able to form a skeleton by direct deposition of silica in a process catalyzed by silicatein. Herein, we isolated biosilicas from five different Atlantic deep-sea sponges Geodia atlantica (GA), Geodia ba...
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Frontiers Media S.A.
2021-05-01
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Series: | Frontiers in Marine Science |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2021.637810/full |
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author | Olesia Dudik Olesia Dudik Sara Amorim Sara Amorim Joana R. Xavier Joana R. Xavier Hans Tore Rapp Tiago H. Silva Tiago H. Silva Ricardo A. Pires Ricardo A. Pires Rui L. Reis Rui L. Reis |
author_facet | Olesia Dudik Olesia Dudik Sara Amorim Sara Amorim Joana R. Xavier Joana R. Xavier Hans Tore Rapp Tiago H. Silva Tiago H. Silva Ricardo A. Pires Ricardo A. Pires Rui L. Reis Rui L. Reis |
author_sort | Olesia Dudik |
collection | DOAJ |
description | Demosponges are a well-known source of a plethora of bioactive compounds. In particular, they are able to form a skeleton by direct deposition of silica in a process catalyzed by silicatein. Herein, we isolated biosilicas from five different Atlantic deep-sea sponges Geodia atlantica (GA), Geodia barretti (GB), Stelletta normani (SN), Axinella infundibuliformis (AI), and Phakellia ventilabrum (PV) to explore the bioactivity and osteogenic capacity of its silica-based materials. We chemically characterized the isolated biosilicas and evaluated them for their bioactivity to deposit Ca and P on their surface (by immersion in simulated body fluid, SBF). GB-, SN-, AI-, and PV-based biosilicas did not generate a stable calcium phosphate (CaP) layer over time in the presence of SBF, however, the GA-derived one was able to form a CaP surface layer (at a Ca/P ratio of ∼1.7, similar to the one observed for hydroxyapatite), that was stable during the 28 days of testing. In addition, no cytotoxicity toward L929 and SaOs2 cells was observed for the GA-based biosilica up to a concentration of 10 mg/mL. Overall, the GA-based biosilica presents the characteristics to be used in the development of biomaterials for bone tissue engineering (BTE). |
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language | English |
last_indexed | 2024-12-21T16:19:42Z |
publishDate | 2021-05-01 |
publisher | Frontiers Media S.A. |
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series | Frontiers in Marine Science |
spelling | doaj.art-10ebead807ec40dd850d26bcc552fa232022-12-21T18:57:36ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-05-01810.3389/fmars.2021.637810637810Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea SpongesOlesia Dudik0Olesia Dudik1Sara Amorim2Sara Amorim3Joana R. Xavier4Joana R. Xavier5Hans Tore Rapp6Tiago H. Silva7Tiago H. Silva8Ricardo A. Pires9Ricardo A. Pires10Rui L. Reis11Rui L. Reis123B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Guimarães, Portugal3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Guimarães, PortugalCIIMAR – Interdisciplinary Centre of Marine and Environmental Research, Terminal de Cruzeiros de Leixões, Matosinhos, PortugalDepartment of Biology and K.G. Jebsen Centre for Deep-sea Research, University of Bergen, Bergen, NorwayDepartment of Biology and K.G. Jebsen Centre for Deep-sea Research, University of Bergen, Bergen, Norway3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Guimarães, Portugal3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Guimarães, Portugal3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Guimarães, PortugalDemosponges are a well-known source of a plethora of bioactive compounds. In particular, they are able to form a skeleton by direct deposition of silica in a process catalyzed by silicatein. Herein, we isolated biosilicas from five different Atlantic deep-sea sponges Geodia atlantica (GA), Geodia barretti (GB), Stelletta normani (SN), Axinella infundibuliformis (AI), and Phakellia ventilabrum (PV) to explore the bioactivity and osteogenic capacity of its silica-based materials. We chemically characterized the isolated biosilicas and evaluated them for their bioactivity to deposit Ca and P on their surface (by immersion in simulated body fluid, SBF). GB-, SN-, AI-, and PV-based biosilicas did not generate a stable calcium phosphate (CaP) layer over time in the presence of SBF, however, the GA-derived one was able to form a CaP surface layer (at a Ca/P ratio of ∼1.7, similar to the one observed for hydroxyapatite), that was stable during the 28 days of testing. In addition, no cytotoxicity toward L929 and SaOs2 cells was observed for the GA-based biosilica up to a concentration of 10 mg/mL. Overall, the GA-based biosilica presents the characteristics to be used in the development of biomaterials for bone tissue engineering (BTE).https://www.frontiersin.org/articles/10.3389/fmars.2021.637810/fullbioactivitybiosilicabioceramicstissue engineeringdeep-sea sponges |
spellingShingle | Olesia Dudik Olesia Dudik Sara Amorim Sara Amorim Joana R. Xavier Joana R. Xavier Hans Tore Rapp Tiago H. Silva Tiago H. Silva Ricardo A. Pires Ricardo A. Pires Rui L. Reis Rui L. Reis Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges Frontiers in Marine Science bioactivity biosilica bioceramics tissue engineering deep-sea sponges |
title | Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges |
title_full | Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges |
title_fullStr | Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges |
title_full_unstemmed | Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges |
title_short | Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges |
title_sort | bioactivity of biosilica obtained from north atlantic deep sea sponges |
topic | bioactivity biosilica bioceramics tissue engineering deep-sea sponges |
url | https://www.frontiersin.org/articles/10.3389/fmars.2021.637810/full |
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