miR-10b regulated by Twist maintains myofibroblasts activities in oral submucous fibrosis
Background/purpose: Oral submucous fibrosis (OSF) is an oral precancerous disorder associated with the habit of areca nut chewing. MiR-10b has been shown to be upregulated in the oral cancer cells and induced by Twist. Our previous work has revealed that Twist participated in the pathogenesis of OSF...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664620300796 |
| _version_ | 1818849733366513664 |
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| author | Chih-Yuan Fang Cheng-Chia Yu Yi-Wen Liao Pei-Ling Hsieh Yoichi Ohiro Pei-Ming Chu Yu-Cheng Huang Chuan-Hang Yu Lo-Lin Tsai |
| author_facet | Chih-Yuan Fang Cheng-Chia Yu Yi-Wen Liao Pei-Ling Hsieh Yoichi Ohiro Pei-Ming Chu Yu-Cheng Huang Chuan-Hang Yu Lo-Lin Tsai |
| author_sort | Chih-Yuan Fang |
| collection | DOAJ |
| description | Background/purpose: Oral submucous fibrosis (OSF) is an oral precancerous disorder associated with the habit of areca nut chewing. MiR-10b has been shown to be upregulated in the oral cancer cells and induced by Twist. Our previous work has revealed that Twist participated in the pathogenesis of OSF and therefore we aimed to investigate whether Twist/miR-10b axis was involved in the activation of myofibroblast in the oral cavity. Methods: The expression levels of miR-10b in OSF tissues and fibrotic buccal mucosal fibroblasts (fBMFs) were examined. Besides, the expression of miR-10b was determined in fBMFs following knockdown of Twist or in BMFs after arecoline stimulation. Myofibroblast activities, including collagen gel contraction, migration and wound healing abilities, as well as the expression of α-SMA were measured in fBMFs treated with miR-10b inhibitor. Last, we investigated whether the effect of Twist overexpression could be reversed by suppression of miR-10b. Results: MiR-10b expression was overexpressed in both OSF tissues and fBMFs. The silence of Twist resulted in the downregulation of miR-10b in fBMFs and arecoline treatment led to an increase of miR-10b in a dose-dependent manner. Inhibition of miR-10b ameliorated the activation of myofibroblasts and the expression of α-SMA. Moreover, we demonstrated that suppression of miR-10b hindered the increased collagen gel contraction caused by Twist overexpression. Conclusion: MiR-10b upregulation in OSF may be due to the stimulation of areca nut, leading to elevated myofibroblast activation. Our findings showed that the areca nut-induced expression of miR-10b was under the regulation of Twist and inhibition of miR-10b may provide a direction for treatment of OSF. |
| first_indexed | 2024-12-19T06:37:56Z |
| format | Article |
| id | doaj.art-10ef8e8191b8450aaf92d8d54eb7164b |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-19T06:37:56Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-10ef8e8191b8450aaf92d8d54eb7164b2022-12-21T20:32:11ZengElsevierJournal of the Formosan Medical Association0929-66462020-07-01119711671173miR-10b regulated by Twist maintains myofibroblasts activities in oral submucous fibrosisChih-Yuan Fang0Cheng-Chia Yu1Yi-Wen Liao2Pei-Ling Hsieh3Yoichi Ohiro4Pei-Ming Chu5Yu-Cheng Huang6Chuan-Hang Yu7Lo-Lin Tsai8School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Division of Oral and Maxillofacial Surgery, Department of Dentistry, Wan Fang Hospital, Taipei Medical University, Taipei, TaiwanInstitute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan; School of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, TaiwanInstitute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan; School of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, TaiwanDepartment of Anatomy, School of Medicine, China Medical University, Taichung, TaiwanDepartment of Oral and Maxillofacial Surgery, Hokkaido University Graduate School of Dental Medicine, Sapporo, JapanDepartment of Anatomy, School of Medicine, China Medical University, Taichung, TaiwanSchool of Medicine, China Medical University, Taichung, TaiwanInstitute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan; School of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan; Corresponding author. School of Dentistry, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung 40201, Taiwan. Fax: +886 4 24759065.School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Division of Oral and Maxillofacial Surgery, Department of Dentistry, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan; Corresponding author. School of Dentistry, Taipei Medical University, No. 250, Wuxing St., Taipei, 11031, Taiwan.Background/purpose: Oral submucous fibrosis (OSF) is an oral precancerous disorder associated with the habit of areca nut chewing. MiR-10b has been shown to be upregulated in the oral cancer cells and induced by Twist. Our previous work has revealed that Twist participated in the pathogenesis of OSF and therefore we aimed to investigate whether Twist/miR-10b axis was involved in the activation of myofibroblast in the oral cavity. Methods: The expression levels of miR-10b in OSF tissues and fibrotic buccal mucosal fibroblasts (fBMFs) were examined. Besides, the expression of miR-10b was determined in fBMFs following knockdown of Twist or in BMFs after arecoline stimulation. Myofibroblast activities, including collagen gel contraction, migration and wound healing abilities, as well as the expression of α-SMA were measured in fBMFs treated with miR-10b inhibitor. Last, we investigated whether the effect of Twist overexpression could be reversed by suppression of miR-10b. Results: MiR-10b expression was overexpressed in both OSF tissues and fBMFs. The silence of Twist resulted in the downregulation of miR-10b in fBMFs and arecoline treatment led to an increase of miR-10b in a dose-dependent manner. Inhibition of miR-10b ameliorated the activation of myofibroblasts and the expression of α-SMA. Moreover, we demonstrated that suppression of miR-10b hindered the increased collagen gel contraction caused by Twist overexpression. Conclusion: MiR-10b upregulation in OSF may be due to the stimulation of areca nut, leading to elevated myofibroblast activation. Our findings showed that the areca nut-induced expression of miR-10b was under the regulation of Twist and inhibition of miR-10b may provide a direction for treatment of OSF.http://www.sciencedirect.com/science/article/pii/S0929664620300796Oral submucous fibrosisMicroRNA-10bTwistMyofibroblast |
| spellingShingle | Chih-Yuan Fang Cheng-Chia Yu Yi-Wen Liao Pei-Ling Hsieh Yoichi Ohiro Pei-Ming Chu Yu-Cheng Huang Chuan-Hang Yu Lo-Lin Tsai miR-10b regulated by Twist maintains myofibroblasts activities in oral submucous fibrosis Journal of the Formosan Medical Association Oral submucous fibrosis MicroRNA-10b Twist Myofibroblast |
| title | miR-10b regulated by Twist maintains myofibroblasts activities in oral submucous fibrosis |
| title_full | miR-10b regulated by Twist maintains myofibroblasts activities in oral submucous fibrosis |
| title_fullStr | miR-10b regulated by Twist maintains myofibroblasts activities in oral submucous fibrosis |
| title_full_unstemmed | miR-10b regulated by Twist maintains myofibroblasts activities in oral submucous fibrosis |
| title_short | miR-10b regulated by Twist maintains myofibroblasts activities in oral submucous fibrosis |
| title_sort | mir 10b regulated by twist maintains myofibroblasts activities in oral submucous fibrosis |
| topic | Oral submucous fibrosis MicroRNA-10b Twist Myofibroblast |
| url | http://www.sciencedirect.com/science/article/pii/S0929664620300796 |
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