Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways
Stem cell homeostasis in plant shoot meristems requires tight coordination between stem cell proliferation and cell differentiation. In Arabidopsis, stem cells express the secreted dodecapeptide CLAVATA3 (CLV3), which signals through the leucine-rich repeat (LRR)-receptor kinase CLAVATA1 (CLV1) and...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-10-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/70934 |
| _version_ | 1811227693105020928 |
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| author | Jenia Schlegel Gregoire Denay Rene Wink Karine Gustavo Pinto Yvonne Stahl Julia Schmid Patrick Blümke Rüdiger GW Simon |
| author_facet | Jenia Schlegel Gregoire Denay Rene Wink Karine Gustavo Pinto Yvonne Stahl Julia Schmid Patrick Blümke Rüdiger GW Simon |
| author_sort | Jenia Schlegel |
| collection | DOAJ |
| description | Stem cell homeostasis in plant shoot meristems requires tight coordination between stem cell proliferation and cell differentiation. In Arabidopsis, stem cells express the secreted dodecapeptide CLAVATA3 (CLV3), which signals through the leucine-rich repeat (LRR)-receptor kinase CLAVATA1 (CLV1) and related CLV1-family members to downregulate expression of the homeodomain transcription factor WUSCHEL (WUS). WUS protein moves from cells below the stem cell domain to the meristem tip and promotes stem cell identity, together with CLV3 expression, generating a negative feedback loop. How stem cell activity in the meristem centre is coordinated with organ initiation and cell differentiation at the periphery is unknown. We show here that the CLE40 gene, encoding a secreted peptide closely related to CLV3, is expressed in the SAM in differentiating cells in a pattern complementary to that of CLV3. CLE40 promotes WUS expression via BAM1, a CLV1-family receptor, and CLE40 expression is in turn repressed in a WUS-dependent manner. Together, CLE40-BAM1-WUS establish a second negative feedback loop. We propose that stem cell homeostasis is achieved through two intertwined pathways that adjust WUS activity and incorporate information on the size of the stem cell domain, via CLV3-CLV1, and on cell differentiation via CLE40-BAM1. |
| first_indexed | 2024-04-12T09:46:11Z |
| format | Article |
| id | doaj.art-10f0ee34285e4c0b881ce5e44e015330 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:46:11Z |
| publishDate | 2021-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-10f0ee34285e4c0b881ce5e44e0153302022-12-22T03:37:56ZengeLife Sciences Publications LtdeLife2050-084X2021-10-011010.7554/eLife.70934Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathwaysJenia Schlegel0https://orcid.org/0000-0003-0434-4479Gregoire Denay1https://orcid.org/0000-0002-8850-3029Rene Wink2Karine Gustavo Pinto3Yvonne Stahl4Julia Schmid5Patrick Blümke6https://orcid.org/0000-0001-7315-6792Rüdiger GW Simon7https://orcid.org/0000-0002-1317-7716Institute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, GermanyInstitute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, GermanyInstitute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, GermanyInstitute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, GermanyInstitute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, GermanyInstitute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, GermanyInstitute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, GermanyInstitute for Developmental Genetics and Cluster of Excellence on Plant Sciences, Heinrich Heine University, Düsseldorf, GermanyStem cell homeostasis in plant shoot meristems requires tight coordination between stem cell proliferation and cell differentiation. In Arabidopsis, stem cells express the secreted dodecapeptide CLAVATA3 (CLV3), which signals through the leucine-rich repeat (LRR)-receptor kinase CLAVATA1 (CLV1) and related CLV1-family members to downregulate expression of the homeodomain transcription factor WUSCHEL (WUS). WUS protein moves from cells below the stem cell domain to the meristem tip and promotes stem cell identity, together with CLV3 expression, generating a negative feedback loop. How stem cell activity in the meristem centre is coordinated with organ initiation and cell differentiation at the periphery is unknown. We show here that the CLE40 gene, encoding a secreted peptide closely related to CLV3, is expressed in the SAM in differentiating cells in a pattern complementary to that of CLV3. CLE40 promotes WUS expression via BAM1, a CLV1-family receptor, and CLE40 expression is in turn repressed in a WUS-dependent manner. Together, CLE40-BAM1-WUS establish a second negative feedback loop. We propose that stem cell homeostasis is achieved through two intertwined pathways that adjust WUS activity and incorporate information on the size of the stem cell domain, via CLV3-CLV1, and on cell differentiation via CLE40-BAM1.https://elifesciences.org/articles/70934shoot meristemstem cellsCLE40CLAVATAsignallingWUS |
| spellingShingle | Jenia Schlegel Gregoire Denay Rene Wink Karine Gustavo Pinto Yvonne Stahl Julia Schmid Patrick Blümke Rüdiger GW Simon Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways eLife shoot meristem stem cells CLE40 CLAVATA signalling WUS |
| title | Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways |
| title_full | Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways |
| title_fullStr | Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways |
| title_full_unstemmed | Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways |
| title_short | Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways |
| title_sort | control of arabidopsis shoot stem cell homeostasis by two antagonistic cle peptide signalling pathways |
| topic | shoot meristem stem cells CLE40 CLAVATA signalling WUS |
| url | https://elifesciences.org/articles/70934 |
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