Gene Expression Profiling of MicroRNAs in HPV-Induced Warts and Normal Skin
<b>: </b>Infection with the human papillomavirus (HPV) is a common occurrence among the global population, with millions of new cases emerging on an annual basis. Dysregulated microRNA (miRNA) expression is increasingly being identified to play a role in a number of different diseases, e...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/12/757 |
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| author | Laith N. AL-Eitan Mansour A. Alghamdi Amneh H. Tarkhan Firas A. Al-Qarqaz |
| author_facet | Laith N. AL-Eitan Mansour A. Alghamdi Amneh H. Tarkhan Firas A. Al-Qarqaz |
| author_sort | Laith N. AL-Eitan |
| collection | DOAJ |
| description | <b>: </b>Infection with the human papillomavirus (HPV) is a common occurrence among the global population, with millions of new cases emerging on an annual basis. Dysregulated microRNA (miRNA) expression is increasingly being identified to play a role in a number of different diseases, especially in the context of high-risk HPV infection. The present study investigated the miRNA expression profiles of warts induced by low-risk HPV. In warts, miR-27b, miR-24-1, miR-3654, miR-647, and miR-1914 were downregulated while miR-612 was upregulated compared to normal skin. Using miRTargetLink Human, experimentally supported evidence was obtained showing that miR-27b targeted the vascular endothelial growth factor C (<i>VEGFC</i>) and CAMP-responsive element binding protein 1 (<i>CREB1</i>) genes. The <i>VEGFC</i> and <i>CREB1</i> genes have been reported to be involved in tumorigenesis and wart formation, respectively. Similarly, the oxidized low-density lipoprotein receptor 1 (<i>OLR1</i>) gene, which plays an important role in the humoral immunity of the skin, and the plexin D1 (<i>PLXND1</i>) gene, which is highly expressed in tumor vasculature, were both found to be common targets of miR-27b, miR-1914, and miR-612. |
| first_indexed | 2024-04-14T00:28:02Z |
| format | Article |
| id | doaj.art-10f446c5b6cc4f81a77225c9cea2156c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-04-14T00:28:02Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-10f446c5b6cc4f81a77225c9cea2156c2022-12-22T02:22:39ZengMDPI AGBiomolecules2218-273X2019-11-0191275710.3390/biom9120757biom9120757Gene Expression Profiling of MicroRNAs in HPV-Induced Warts and Normal SkinLaith N. AL-Eitan0Mansour A. Alghamdi1Amneh H. Tarkhan2Firas A. Al-Qarqaz3Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid 22110, JordanDepartment of Anatomy, College of Medicine, King Khalid University, Abha 61421, Saudi ArabiaDepartment of Applied Biological Sciences, Jordan University of Science and Technology, Irbid 22110, JordanDepartment of Internal Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan<b>: </b>Infection with the human papillomavirus (HPV) is a common occurrence among the global population, with millions of new cases emerging on an annual basis. Dysregulated microRNA (miRNA) expression is increasingly being identified to play a role in a number of different diseases, especially in the context of high-risk HPV infection. The present study investigated the miRNA expression profiles of warts induced by low-risk HPV. In warts, miR-27b, miR-24-1, miR-3654, miR-647, and miR-1914 were downregulated while miR-612 was upregulated compared to normal skin. Using miRTargetLink Human, experimentally supported evidence was obtained showing that miR-27b targeted the vascular endothelial growth factor C (<i>VEGFC</i>) and CAMP-responsive element binding protein 1 (<i>CREB1</i>) genes. The <i>VEGFC</i> and <i>CREB1</i> genes have been reported to be involved in tumorigenesis and wart formation, respectively. Similarly, the oxidized low-density lipoprotein receptor 1 (<i>OLR1</i>) gene, which plays an important role in the humoral immunity of the skin, and the plexin D1 (<i>PLXND1</i>) gene, which is highly expressed in tumor vasculature, were both found to be common targets of miR-27b, miR-1914, and miR-612.https://www.mdpi.com/2218-273X/9/12/757hpvwartmicrornarna-seq |
| spellingShingle | Laith N. AL-Eitan Mansour A. Alghamdi Amneh H. Tarkhan Firas A. Al-Qarqaz Gene Expression Profiling of MicroRNAs in HPV-Induced Warts and Normal Skin Biomolecules hpv wart microrna rna-seq |
| title | Gene Expression Profiling of MicroRNAs in HPV-Induced Warts and Normal Skin |
| title_full | Gene Expression Profiling of MicroRNAs in HPV-Induced Warts and Normal Skin |
| title_fullStr | Gene Expression Profiling of MicroRNAs in HPV-Induced Warts and Normal Skin |
| title_full_unstemmed | Gene Expression Profiling of MicroRNAs in HPV-Induced Warts and Normal Skin |
| title_short | Gene Expression Profiling of MicroRNAs in HPV-Induced Warts and Normal Skin |
| title_sort | gene expression profiling of micrornas in hpv induced warts and normal skin |
| topic | hpv wart microrna rna-seq |
| url | https://www.mdpi.com/2218-273X/9/12/757 |
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