A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration
Vegetative desiccation tolerance, or the ability to survive the loss of ~95% relative water content (RWC), is rare in angiosperms, with these being commonly called resurrection plants. It is a complex multigenic and multi-factorial trait, with its understanding requiring a comprehensive systems biol...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/11/11/1242 |
| _version_ | 1827676203208671232 |
|---|---|
| author | Hawwa Gabier David L. Tabb Jill M. Farrant Mohamed Suhail Rafudeen |
| author_facet | Hawwa Gabier David L. Tabb Jill M. Farrant Mohamed Suhail Rafudeen |
| author_sort | Hawwa Gabier |
| collection | DOAJ |
| description | Vegetative desiccation tolerance, or the ability to survive the loss of ~95% relative water content (RWC), is rare in angiosperms, with these being commonly called resurrection plants. It is a complex multigenic and multi-factorial trait, with its understanding requiring a comprehensive systems biology approach. The aim of the current study was to conduct a label-free proteomic analysis of leaves of the resurrection plant <i>Xerophyta schlechteri</i> in response to desiccation. A targeted metabolomics approach was validated and correlated to the proteomics, contributing the missing link in studies on this species. Three physiological stages were identified: an early response to drying, during which the leaf tissues declined from full turgor to a RWC of ~80–70%, a mid-response in which the RWC declined to 40% and a late response where the tissues declined to 10% RWC. We identified 517 distinct proteins that were differentially expressed, of which 253 proteins were upregulated and 264 were downregulated in response to the three drying stages. Metabolomics analyses, which included monitoring the levels of a selection of phytohormones, amino acids, sugars, sugar alcohols, fatty acids and organic acids in response to dehydration, correlated with some of the proteomic differences, giving insight into the biological processes apparently involved in desiccation tolerance in this species. |
| first_indexed | 2024-03-10T05:20:23Z |
| format | Article |
| id | doaj.art-10f6ff511cf04c04a4505fbadf7b76ac |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T05:20:23Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-10f6ff511cf04c04a4505fbadf7b76ac2023-11-23T00:04:57ZengMDPI AGLife2075-17292021-11-011111124210.3390/life11111242A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during DehydrationHawwa Gabier0David L. Tabb1Jill M. Farrant2Mohamed Suhail Rafudeen3Department of Molecular and Cell Biology, University of Cape Town, Private Bag X3, Rondebosch 7701, South AfricaInstitute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town 7700, South AfricaDepartment of Molecular and Cell Biology, University of Cape Town, Private Bag X3, Rondebosch 7701, South AfricaDepartment of Molecular and Cell Biology, University of Cape Town, Private Bag X3, Rondebosch 7701, South AfricaVegetative desiccation tolerance, or the ability to survive the loss of ~95% relative water content (RWC), is rare in angiosperms, with these being commonly called resurrection plants. It is a complex multigenic and multi-factorial trait, with its understanding requiring a comprehensive systems biology approach. The aim of the current study was to conduct a label-free proteomic analysis of leaves of the resurrection plant <i>Xerophyta schlechteri</i> in response to desiccation. A targeted metabolomics approach was validated and correlated to the proteomics, contributing the missing link in studies on this species. Three physiological stages were identified: an early response to drying, during which the leaf tissues declined from full turgor to a RWC of ~80–70%, a mid-response in which the RWC declined to 40% and a late response where the tissues declined to 10% RWC. We identified 517 distinct proteins that were differentially expressed, of which 253 proteins were upregulated and 264 were downregulated in response to the three drying stages. Metabolomics analyses, which included monitoring the levels of a selection of phytohormones, amino acids, sugars, sugar alcohols, fatty acids and organic acids in response to dehydration, correlated with some of the proteomic differences, giving insight into the biological processes apparently involved in desiccation tolerance in this species.https://www.mdpi.com/2075-1729/11/11/1242dehydrationdesiccation toleranceplant proteomicslabel-free quantificationmetabolomicsresurrection plant |
| spellingShingle | Hawwa Gabier David L. Tabb Jill M. Farrant Mohamed Suhail Rafudeen A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration Life dehydration desiccation tolerance plant proteomics label-free quantification metabolomics resurrection plant |
| title | A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration |
| title_full | A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration |
| title_fullStr | A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration |
| title_full_unstemmed | A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration |
| title_short | A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration |
| title_sort | label free proteomic and complementary metabolomic analysis of leaves of the resurrection plant i xerophyta i i schlechteri i during dehydration |
| topic | dehydration desiccation tolerance plant proteomics label-free quantification metabolomics resurrection plant |
| url | https://www.mdpi.com/2075-1729/11/11/1242 |
| work_keys_str_mv | AT hawwagabier alabelfreeproteomicandcomplementarymetabolomicanalysisofleavesoftheresurrectionplantixerophytaiischlechteriiduringdehydration AT davidltabb alabelfreeproteomicandcomplementarymetabolomicanalysisofleavesoftheresurrectionplantixerophytaiischlechteriiduringdehydration AT jillmfarrant alabelfreeproteomicandcomplementarymetabolomicanalysisofleavesoftheresurrectionplantixerophytaiischlechteriiduringdehydration AT mohamedsuhailrafudeen alabelfreeproteomicandcomplementarymetabolomicanalysisofleavesoftheresurrectionplantixerophytaiischlechteriiduringdehydration AT hawwagabier labelfreeproteomicandcomplementarymetabolomicanalysisofleavesoftheresurrectionplantixerophytaiischlechteriiduringdehydration AT davidltabb labelfreeproteomicandcomplementarymetabolomicanalysisofleavesoftheresurrectionplantixerophytaiischlechteriiduringdehydration AT jillmfarrant labelfreeproteomicandcomplementarymetabolomicanalysisofleavesoftheresurrectionplantixerophytaiischlechteriiduringdehydration AT mohamedsuhailrafudeen labelfreeproteomicandcomplementarymetabolomicanalysisofleavesoftheresurrectionplantixerophytaiischlechteriiduringdehydration |