Profound Perturbation in the Metabolome of a Canine Obesity and Metabolic Disorder Model
Canine models are increasingly being used in metabolic studies due to their physiological similarity with humans. The present study aimed to identify changes in metabolic pathways and biomarkers with potential clinical utility in a canine model of obesity and metabolic disorders induced by a high-fa...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.849060/full |
| _version_ | 1811305927275446272 |
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| author | Weiyi Qu Weiyi Qu Ze Chen Ze Chen Xing Hu Xing Hu Toujun Zou Toujun Zou Yongping Huang Yongping Huang Yanyan Zhang Yanyan Zhang Yufeng Hu Yufeng Hu Song Tian Song Tian Juan Wan Juan Wan Rufang Liao Lan Bai Lan Bai Jinhua Xue Jinhua Xue Yi Ding Manli Hu Xiao-Jing Zhang Xiao-Jing Zhang Xin Zhang Jingjing Zhao Xu Cheng Xu Cheng Zhi-Gang She Zhi-Gang She Hongliang Li Hongliang Li Hongliang Li |
| author_facet | Weiyi Qu Weiyi Qu Ze Chen Ze Chen Xing Hu Xing Hu Toujun Zou Toujun Zou Yongping Huang Yongping Huang Yanyan Zhang Yanyan Zhang Yufeng Hu Yufeng Hu Song Tian Song Tian Juan Wan Juan Wan Rufang Liao Lan Bai Lan Bai Jinhua Xue Jinhua Xue Yi Ding Manli Hu Xiao-Jing Zhang Xiao-Jing Zhang Xin Zhang Jingjing Zhao Xu Cheng Xu Cheng Zhi-Gang She Zhi-Gang She Hongliang Li Hongliang Li Hongliang Li |
| author_sort | Weiyi Qu |
| collection | DOAJ |
| description | Canine models are increasingly being used in metabolic studies due to their physiological similarity with humans. The present study aimed to identify changes in metabolic pathways and biomarkers with potential clinical utility in a canine model of obesity and metabolic disorders induced by a high-fat diet (HFD). Eighteen male beagles were included in this study, 9 of which were fed a HFD for 24 weeks, and the remaining 9 were fed normal chow (NC) during the same period. Plasma and urine samples were collected at weeks 12 and 24 for untargeted metabolomic analysis. Dogs fed a HFD showed a gradual body weight increase during the feeding period and had hyperlipidemia, increased leukocyte counts, and impaired insulin sensitivity at week 24. Plasma and urine metabonomics analysis displayed clear separations between the HFD-fed and NC-fed dogs. A total of 263 plasma metabolites varied between the two groups, including stearidonic acid, linolenic acid, carnitine, long-chain ceramide, 3-methylxanthine, and theophylline, which are mainly engaged in fatty acid metabolism, sphingolipid metabolism, and caffeine metabolism. A total of 132 urine metabolites related to HFD-induced obesity and metabolic disorders were identified, including 3-methylxanthine, theophylline, pyridoxal 5’-phosphate, and harmine, which participate in pathways such as caffeine metabolism and vitamin digestion and absorption. Eight metabolites with increased abundance (e.g., 3-methylxanthine, theophylline, and harmine) and 4 metabolites with decreased abundance (e.g., trigonelline) in both the plasma and urine of the HFD-fed dogs were identified. In conclusion, the metabolomic analysis revealed molecular events underlying a canine HFD model and identified several metabolites as potential targets for the prevention and treatment of obesity-related metabolic disorders. |
| first_indexed | 2024-04-13T08:34:09Z |
| format | Article |
| id | doaj.art-10fac5a368c14cbc8f6c0292e3f346f9 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-13T08:34:09Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-10fac5a368c14cbc8f6c0292e3f346f92022-12-22T02:54:08ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-04-011310.3389/fendo.2022.849060849060Profound Perturbation in the Metabolome of a Canine Obesity and Metabolic Disorder ModelWeiyi Qu0Weiyi Qu1Ze Chen2Ze Chen3Xing Hu4Xing Hu5Toujun Zou6Toujun Zou7Yongping Huang8Yongping Huang9Yanyan Zhang10Yanyan Zhang11Yufeng Hu12Yufeng Hu13Song Tian14Song Tian15Juan Wan16Juan Wan17Rufang Liao18Lan Bai19Lan Bai20Jinhua Xue21Jinhua Xue22Yi Ding23Manli Hu24Xiao-Jing Zhang25Xiao-Jing Zhang26Xin Zhang27Jingjing Zhao28Xu Cheng29Xu Cheng30Zhi-Gang She31Zhi-Gang She32Hongliang Li33Hongliang Li34Hongliang Li35Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaDepartment of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Urology, First Affiliated Hospital of Gannan Medical University, Ganzhou, ChinaGannan Innovation and Translational Medicine Research Institute, Gannan Medical University, Ganzhou, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaCollege of Life Sciences, Wuhan University, Wuhan, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaGannan Innovation and Translational Medicine Research Institute, Gannan Medical University, Ganzhou, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaSchool of Basic Medical Science, Wuhan University, Wuhan, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaDepartment of Radiology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaGannan Innovation and Translational Medicine Research Institute, Gannan Medical University, Ganzhou, China0Department of Pathophysiology, School of Basic Medical Sciences, Gannan Medical University, Ganzhou, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaGannan Innovation and Translational Medicine Research Institute, Gannan Medical University, Ganzhou, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaSchool of Basic Medical Science, Wuhan University, Wuhan, ChinaGannan Innovation and Translational Medicine Research Institute, Gannan Medical University, Ganzhou, China1Department of Cardiology, Tongren Hospital of Wuhan University and Wuhan Third Hospital, Wuhan, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, ChinaInstitute of Model Animal, Wuhan University, Wuhan, ChinaSchool of Basic Medical Science, Wuhan University, Wuhan, ChinaCanine models are increasingly being used in metabolic studies due to their physiological similarity with humans. The present study aimed to identify changes in metabolic pathways and biomarkers with potential clinical utility in a canine model of obesity and metabolic disorders induced by a high-fat diet (HFD). Eighteen male beagles were included in this study, 9 of which were fed a HFD for 24 weeks, and the remaining 9 were fed normal chow (NC) during the same period. Plasma and urine samples were collected at weeks 12 and 24 for untargeted metabolomic analysis. Dogs fed a HFD showed a gradual body weight increase during the feeding period and had hyperlipidemia, increased leukocyte counts, and impaired insulin sensitivity at week 24. Plasma and urine metabonomics analysis displayed clear separations between the HFD-fed and NC-fed dogs. A total of 263 plasma metabolites varied between the two groups, including stearidonic acid, linolenic acid, carnitine, long-chain ceramide, 3-methylxanthine, and theophylline, which are mainly engaged in fatty acid metabolism, sphingolipid metabolism, and caffeine metabolism. A total of 132 urine metabolites related to HFD-induced obesity and metabolic disorders were identified, including 3-methylxanthine, theophylline, pyridoxal 5’-phosphate, and harmine, which participate in pathways such as caffeine metabolism and vitamin digestion and absorption. Eight metabolites with increased abundance (e.g., 3-methylxanthine, theophylline, and harmine) and 4 metabolites with decreased abundance (e.g., trigonelline) in both the plasma and urine of the HFD-fed dogs were identified. In conclusion, the metabolomic analysis revealed molecular events underlying a canine HFD model and identified several metabolites as potential targets for the prevention and treatment of obesity-related metabolic disorders.https://www.frontiersin.org/articles/10.3389/fendo.2022.849060/fullmetabolomicsobesitymetabolic disorderhigh-fat dietmetabolic profilesenergy intake |
| spellingShingle | Weiyi Qu Weiyi Qu Ze Chen Ze Chen Xing Hu Xing Hu Toujun Zou Toujun Zou Yongping Huang Yongping Huang Yanyan Zhang Yanyan Zhang Yufeng Hu Yufeng Hu Song Tian Song Tian Juan Wan Juan Wan Rufang Liao Lan Bai Lan Bai Jinhua Xue Jinhua Xue Yi Ding Manli Hu Xiao-Jing Zhang Xiao-Jing Zhang Xin Zhang Jingjing Zhao Xu Cheng Xu Cheng Zhi-Gang She Zhi-Gang She Hongliang Li Hongliang Li Hongliang Li Profound Perturbation in the Metabolome of a Canine Obesity and Metabolic Disorder Model Frontiers in Endocrinology metabolomics obesity metabolic disorder high-fat diet metabolic profiles energy intake |
| title | Profound Perturbation in the Metabolome of a Canine Obesity and Metabolic Disorder Model |
| title_full | Profound Perturbation in the Metabolome of a Canine Obesity and Metabolic Disorder Model |
| title_fullStr | Profound Perturbation in the Metabolome of a Canine Obesity and Metabolic Disorder Model |
| title_full_unstemmed | Profound Perturbation in the Metabolome of a Canine Obesity and Metabolic Disorder Model |
| title_short | Profound Perturbation in the Metabolome of a Canine Obesity and Metabolic Disorder Model |
| title_sort | profound perturbation in the metabolome of a canine obesity and metabolic disorder model |
| topic | metabolomics obesity metabolic disorder high-fat diet metabolic profiles energy intake |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.849060/full |
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